CAJ | 학술논문

目的探讨胶质瘤干祖细胞体外诱导恶性转化的树突状细胞株的建立及其特征分析.方法取表达绿色荧光蛋白( EGFP)的小鼠骨髓腔冲洗细胞,用树突状细胞诱导培养方法,与表达红色荧光蛋白( RFP)的人胶质瘤干祖细胞SU3共培养,从单克隆增殖能力强的EGFP(+)永生化细胞中随机选取1株检测相关分子标志物,行染色体分析和致瘤试验. 结果被鉴定分析的1株永生化EGFP(+)细胞形态为树突状,DC标志蛋白CD11c、CD80、信号调节蛋白α( SIRP-α)均高表达,此外表达宿主鼠源性β-肌动蛋白和EGFP;且兼具高增殖、侵袭和移植致癌的潜能;染色体核型为异倍体. 结论在体外共培养系统中,成功地建立了1株被胶质瘤干祖细胞SU3诱导恶性转化的永生化树突状细胞株,有望作为工具细胞进一步用于非可控性炎症细胞的相关研究.
목적 탐토효질류간조세포체외유도악성전화적수돌상세포주적건립급기특정분석.방법 취표체록색형광단백( EGFP)적소서골수강충세세포,용수돌상세포유도배양방법,여표체홍색형광단백( RFP)적인효질류간조세포SU3공배양,종단극륭증식능력강적EGFP(+)영생화세포중수궤선취1주검측상관분자표지물,행염색체분석화치류시험. 결과 피감정분석적1주영생화EGFP(+)세포형태위수돌상,DC표지단백CD11c、CD80、신호조절단백α( SIRP-α)균고표체,차외표체숙주서원성β-기동단백화EGFP;차겸구고증식、침습화이식치암적잠능;염색체핵형위이배체. 결론 재체외공배양계통중,성공지건립료1주피효질류간조세포SU3유도악성전화적영생화수돌상세포주,유망작위공구세포진일보용우비가공성염증세포적상관연구.
Objective To establish a malignant transformed cell line by in vitro induction of dendritic cells with glioma stem progenitor cells and to analyze their characteristics.Methods Bone marrow cells were collected from Foxn1nu.B6-CAG-EGFP/SU heterozygous mice expressing enhanced green fluorescent protein ( EGFP) by flushing bone marrow cavity with PBS and cultured by using DC culturing method.Then, the cells were co-cultured with glioma stem progenitor cells SU3 expressing red fluorescent protein ( RFP ) for the con-struction of immortalized cells expressing EGFP.An immortalized cell strain was randomly selected and analyzed by several assays for the identification of DC molecular markers, detection of chromosome karyotype and analysis of tumorigenicity.Results The immortalized cell strain with typical dendritic morphology was named SU3-ih-DCTC (SU3-induced host dendritic cells transformed tumor cell).High levels of DC marker proteins (CD11c and CD80) and signal regulated protein alpha (SIRP-α) were detected in the SU3-ihDCTC strain.Moreover, the cell line also expressed host mice evidence marker beta actin (β-actin) and EGFP.The SU3-ihDCTC strain was a highly proliferative and invasive cell with carcinogenic potential.The chromosome karyotype of the strain was heteroploid.Conclusion A malignant transformed immortalized dendritic cell line, SU3-ihDCTC, was successfully established by in vitro induction of dendritic cells with glioma stem progenitor cells SU3, which would be useful for further investigation on non-resolving inflammatory cells.