CAJ | 학술논문

目的:探讨剔除Bcl-2表达对胃癌HGC-27细胞凋亡、增殖及5-氟尿嘧啶(5-FU)敏感性的影响.方法:将 HGC-27 细胞分为 5 组:空白对照组、阴性对照组(对照 siRNA )、干扰组( Bcl-2 siRNA )、5-FU 组( 5-FU )、联合组( Bcl-2 siRNA 与 5-FU );通过流式细胞术、MTT 方法检测 HGC-27 细胞凋亡、增殖水平,用 ELISA 法测定半胱氨酸蛋白酶的活力及 Cytochrome C 释放水平. 结果:转染 Bcl-2 siRNA 能提高HGC-27 细胞凋亡率,降低肿瘤细胞的增殖率;相对于 5-FU 单独处理组,转染 Bcl-2 siRNA 与 5-FU 联合组的细胞凋亡水平明显上升, 细胞增殖率显著降低,Caspase-3 和 Caspase-9 活力显著升高,Cytochrome C释放水平亦显著增加(P < 0.01). 结论:剔除Bcl-2基因能诱导HGC-27细胞凋亡,抑制胃癌细胞增殖,增强5-FU 的敏感性.
목적:탐토척제Bcl-2표체대위암HGC-27세포조망、증식급5-불뇨밀정(5-FU)민감성적영향.방법:장 HGC-27 세포분위 5 조:공백대조조、음성대조조(대조 siRNA )、간우조( Bcl-2 siRNA )、5-FU 조( 5-FU )、연합조( Bcl-2 siRNA 여 5-FU );통과류식세포술、MTT 방법검측 HGC-27 세포조망、증식수평,용 ELISA 법측정반광안산단백매적활력급 Cytochrome C 석방수평. 결과:전염 Bcl-2 siRNA 능제고HGC-27 세포조망솔,강저종류세포적증식솔;상대우 5-FU 단독처리조,전염 Bcl-2 siRNA 여 5-FU 연합조적세포조망수평명현상승, 세포증식솔현저강저,Caspase-3 화 Caspase-9 활력현저승고,Cytochrome C석방수평역현저증가(P < 0.01). 결론:척제Bcl-2기인능유도HGC-27세포조망,억제위암세포증식,증강5-FU 적민감성.
Objective To investigate the effect of Bcl-2 gene knockdown on apotosis , proliferation and drug sensitivity of gastric carcinoma HGC-27 cells. Methods The HGC-27 cells were divided into five groups:the untreated control group , the control siRNA group , the specific siRNA targeting Bcl-2 gene group , 5-FU treated group and the combination group (Bcl-2 siRNA and 5-FU treatment). Then flow cytometry and MTT assays were performed to detect the apoptosis and proliferation of HGC-27 cells. The cysteine protease activityand Cytochrome C release level were tested by ELISA method. Results Bcl-2 knockdown enhanced apoptosis and inhibited proliferation of HGC-27 cells. Comparedwith the 5-FU-treated group , the cell apoptosis level, activities of Caspase-3 and Caspase-9, plasma Cytochrome C were significantly increased in the combination group(P <0.01). Conclusion Bcl-2 gene knockdown induced apoptosis, inhibited cell proliferation and enhanced the drug sensitivity of 5-FU in gastric cancer cells , which might be considered as a potential therapeutic strategy forgastric carcinoma.