CAJ | 학술논문

在无溶剂体系中以大孔树脂NKA吸附固定的褶皱假丝酵母脂肪酶(Candida rugosa lipase)为催化剂,以脂肪酸为酰基供体,酶法合成香茅醇脂肪酸酯.考察了褶皱假丝酵母脂肪酶对酰基供体的选择性,以及反应温度、摇床转速、酶用量、底物比例及微量水添加等对酯化反应的影响,建立了无溶剂中香茅醇酯的酶法合成工艺.研究表明,在酸醇物质的量比为1,摇床转速为200 r/min,固定化酶用量为1 250 U/L,反应温度为50℃,微量水添加量为4μL的条件下,反应10 h,月桂酸香茅酯及油酸香茅酯的转化率分别可达95.6%和87.6%,明显高于在有机溶剂体系中的酯化率.
재무용제체계중이대공수지NKA흡부고정적습추가사효모지방매(Candida rugosa lipase)위최화제,이지방산위선기공체,매법합성향모순지방산지.고찰료습추가사효모지방매대선기공체적선택성,이급반응온도、요상전속、매용량、저물비례급미량수첨가등대지화반응적영향,건립료무용제중향모순지적매법합성공예.연구표명,재산순물질적량비위1,요상전속위200 r/min,고정화매용량위1 250 U/L,반응온도위50℃,미량수첨가량위4μL적조건하,반응10 h,월계산향모지급유산향모지적전화솔분별가체95.6%화87.6%,명현고우재유궤용제체계중적지화솔.
The enzymatic synthesis of citronellyl esters by esterification of citronellol and fatty acids in a solvent-free system was discussed using Candida rugosa lipase (CRL) absorbed on macroporous resin NKA as catalyst. The conditions of enzymatic synthesis of citronellyl esters was optimized, including the selectivity of acyl donor, reaction temperature, enzyme dosage, molar ratio of substrate and micro-water addition. The results showed that under the conditions of molar ratio of acid to alcohol 1, agitation speed 200 r/min, reaction temperature 50℃, the concentration of immobilized lipase 1 250 U/L, the addition of water 4μL, citronellyl laurate and citronellyl oleate were achieved with esterification rate of 95.6% and 87.6%, respectively, after 10 h of reaction, which was more superior than that in organic solvent system.