福建农业科技
福建農業科技
복건농업과기
Fujian Agricultural Science and Technology
2015年
7期
10-15,16
,共7页
抑草微生物%抑草机理%菌株鉴定
抑草微生物%抑草機理%菌株鑒定
억초미생물%억초궤리%균주감정
Grass-depressing microorganism%grass-depressing mechanism%strain identification
以从化感水稻根际土壤筛选到的1株具有抑草能力的菌株A为研究对象,通过形态学及分子生物学方法鉴定表明该菌属于曲霉属,该菌株对稗草的抑制率高达70%,菌株A发酵液在稀释100、200倍条件下能明显抑制稗草根系细胞内关键酶与保护酶活力,而胞内 MDA含量增加20%以上。
以從化感水稻根際土壤篩選到的1株具有抑草能力的菌株A為研究對象,通過形態學及分子生物學方法鑒定錶明該菌屬于麯黴屬,該菌株對稗草的抑製率高達70%,菌株A髮酵液在稀釋100、200倍條件下能明顯抑製稗草根繫細胞內關鍵酶與保護酶活力,而胞內 MDA含量增加20%以上。
이종화감수도근제토양사선도적1주구유억초능력적균주A위연구대상,통과형태학급분자생물학방법감정표명해균속우곡매속,해균주대패초적억제솔고체70%,균주A발효액재희석100、200배조건하능명현억제패초근계세포내관건매여보호매활력,이포내 MDA함량증가20%이상。
This paper used a train A,which was screened from rhizosphere soil of allelopathic rice and proved to have grass-depressing capacity as the study objective,identified the strain A by the morphology and molecular biology identification, showing it belongs to genus of aspergillus. Through measurement,the grass-depressing rate of the stain to barnyard grass was as high as 70%. Further investigation proved that the fermentation liquor of strain-A after diluted to 100 and 200 times could restrain the activity of key enzymes and protective enzyme in the root cells of barnyard grass during the training process,and increase the content of intracellular MDA by more than 20%.