四川医学
四川醫學
사천의학
Sichuan Medical Journal
2015年
9期
1261-1263,1264
,共4页
华蟾素%厄洛替尼%A549细胞%凋亡%Caspase-3
華蟾素%阨洛替尼%A549細胞%凋亡%Caspase-3
화섬소%액락체니%A549세포%조망%Caspase-3
cinobuotalin%erlotinib%apoptosis%caspase-3
目的：探讨华蟾素联合EGFR抑制剂厄洛替尼对肺腺癌A549细胞株生长抑制和凋亡诱导作用。方法华蟾素、厄洛替尼单独或联合干预细胞48h后,四甲基偶氮唑盐(MTT)比色法测定细胞抑制率；Hoechst33258染色法检测细胞凋亡和流式细胞学检测药物作用周期；Western Blot检测Caspase-3蛋白表达情况。结果①华蟾素联合厄洛替尼组,相比单药组明显抑制A549细胞的生长,呈时间、剂量依赖性；②联合用药组细胞凋亡率明显高于单药组(P<0．01),S期细胞比例明显减少(P<0．01),G0/G1期细胞比例明显增加(P<0．01),表现出协同作用；③联合用药组Caspase-3蛋白表达明显升高(P<0．01)。结论华蟾素和厄洛替尼联合具有协同抑制肺癌细胞生长,其协同作用的可能机制是通过促进凋亡、增强G 0-G 1期阻滞和上调Caspase-3蛋白的表达。
目的：探討華蟾素聯閤EGFR抑製劑阨洛替尼對肺腺癌A549細胞株生長抑製和凋亡誘導作用。方法華蟾素、阨洛替尼單獨或聯閤榦預細胞48h後,四甲基偶氮唑鹽(MTT)比色法測定細胞抑製率；Hoechst33258染色法檢測細胞凋亡和流式細胞學檢測藥物作用週期；Western Blot檢測Caspase-3蛋白錶達情況。結果①華蟾素聯閤阨洛替尼組,相比單藥組明顯抑製A549細胞的生長,呈時間、劑量依賴性；②聯閤用藥組細胞凋亡率明顯高于單藥組(P<0．01),S期細胞比例明顯減少(P<0．01),G0/G1期細胞比例明顯增加(P<0．01),錶現齣協同作用；③聯閤用藥組Caspase-3蛋白錶達明顯升高(P<0．01)。結論華蟾素和阨洛替尼聯閤具有協同抑製肺癌細胞生長,其協同作用的可能機製是通過促進凋亡、增彊G 0-G 1期阻滯和上調Caspase-3蛋白的錶達。
목적：탐토화섬소연합EGFR억제제액락체니대폐선암A549세포주생장억제화조망유도작용。방법화섬소、액락체니단독혹연합간예세포48h후,사갑기우담서염(MTT)비색법측정세포억제솔；Hoechst33258염색법검측세포조망화류식세포학검측약물작용주기；Western Blot검측Caspase-3단백표체정황。결과①화섬소연합액락체니조,상비단약조명현억제A549세포적생장,정시간、제량의뢰성；②연합용약조세포조망솔명현고우단약조(P<0．01),S기세포비례명현감소(P<0．01),G0/G1기세포비례명현증가(P<0．01),표현출협동작용；③연합용약조Caspase-3단백표체명현승고(P<0．01)。결론화섬소화액락체니연합구유협동억제폐암세포생장,기협동작용적가능궤제시통과촉진조망、증강G 0-G 1기조체화상조Caspase-3단백적표체。
Objective To explore the effects of cinobuotalin combined with erlotinib on apoptosis and expression of caspase-3 in lung cancer A549 cell. Methods A549 cells were treated with cinobuotalin,and erlotinib alone or in combination for 48 hours. cell inhibition rate was detected by methylthiazolyl tetrazolium(MTT), the apoptosis rate were determined through Ho-echst33258 staining method and cell cycle were measured by a flow cytometer, and the expression of Caspase-3, were determined by Western Blot. Results The growth inhibition rates and apoptosis rates in the group D were much higher than the group treated by drug alone(P<0. 01). In contrast to each monotherapy group,S stage cell ratio decreased and G0/G1stage cell ratio increased (P<0. 05),in combined treament group D. However the combination of Cinobuotalin and Erlotinib results in down regulation of G 0-G 1 arrest and G 2-M arrest, suggesting that the effect of these two drugs cooperate with each other. The expression of Caspase-3 up-regulated at in the combined treatment group D, and was statistically different from the monotherapy group(P<0. 01). Con-clusion The results suggest that combination treatment with cinobuotalin,and erlotinib can improve therapeutic efficacy against A549 cells,owing to inducing apoptosis 、G 1 phase arrest and up-regulated expressing Caspase-3.
