四川医学
四川醫學
사천의학
Sichuan Medical Journal
2015年
9期
1240-1243
,共4页
秦亚录%许锋成%余朝萍%吴多江%赖敏%龚厚文%尹晓华%杨静%曾秀娟%晏明君%曾洪燕%刘天虎
秦亞錄%許鋒成%餘朝萍%吳多江%賴敏%龔厚文%尹曉華%楊靜%曾秀娟%晏明君%曾洪燕%劉天虎
진아록%허봉성%여조평%오다강%뢰민%공후문%윤효화%양정%증수연%안명군%증홍연%류천호
Notch-2,3,4/Jagged-1%淋巴细胞%风湿性心脏病%IL-1β%IL-8%TNF-α
Notch-2,3,4/Jagged-1%淋巴細胞%風濕性心髒病%IL-1β%IL-8%TNF-α
Notch-2,3,4/Jagged-1%림파세포%풍습성심장병%IL-1β%IL-8%TNF-α
Notch-2,3,4 /Jagged-1%lymphocytes%rheumatic heart disease%interleukin-1β%interleukin-8%tumor necrosis factor-α
目的：探讨Notch-2、Notch-3、Notch-4和Jagged-1在风湿性心脏病患者外周血淋巴细胞上表达与 IL-1β、IL-8、TNF-α的水平及意义。方法风湿性心脏病患者38例与健康者37例(对照组)采用流式细胞术检测外周血淋巴细胞上Notch-2、Notch-3、Notch-4和Jagged-1的表达水平,酶联免疫吸附法测定IL-1β、IL-8、TNF-α水平。结果 Notch-2、Notch-3、Notch-4/Jagged-1在风湿性心脏病患者外周血淋巴细胞表达水平明显高于对照组,差异有统计学意义( P<0．05)。风湿性心脏病患者外周血IL-1β、IL-8、TNF-α水平高于对照组,差异有统计学意义(P<0．05),Notch-2、Notch-3、Notch-4/Jagged-1在风湿性心脏病患者外周血淋巴细胞表达水平与风湿性心脏病患者外周血IL-1β、IL-8、TNF-α水平呈正相关(P<0．05)。结论风湿性心脏病患者Notch-2、Notch-3、Notch-4/Jagged-1在风湿性心脏病患者外周血淋巴细胞上的表达,影响了IL-1β、IL-8、TNF-α水平,导致免疫功能紊乱。
目的：探討Notch-2、Notch-3、Notch-4和Jagged-1在風濕性心髒病患者外週血淋巴細胞上錶達與 IL-1β、IL-8、TNF-α的水平及意義。方法風濕性心髒病患者38例與健康者37例(對照組)採用流式細胞術檢測外週血淋巴細胞上Notch-2、Notch-3、Notch-4和Jagged-1的錶達水平,酶聯免疫吸附法測定IL-1β、IL-8、TNF-α水平。結果 Notch-2、Notch-3、Notch-4/Jagged-1在風濕性心髒病患者外週血淋巴細胞錶達水平明顯高于對照組,差異有統計學意義( P<0．05)。風濕性心髒病患者外週血IL-1β、IL-8、TNF-α水平高于對照組,差異有統計學意義(P<0．05),Notch-2、Notch-3、Notch-4/Jagged-1在風濕性心髒病患者外週血淋巴細胞錶達水平與風濕性心髒病患者外週血IL-1β、IL-8、TNF-α水平呈正相關(P<0．05)。結論風濕性心髒病患者Notch-2、Notch-3、Notch-4/Jagged-1在風濕性心髒病患者外週血淋巴細胞上的錶達,影響瞭IL-1β、IL-8、TNF-α水平,導緻免疫功能紊亂。
목적：탐토Notch-2、Notch-3、Notch-4화Jagged-1재풍습성심장병환자외주혈림파세포상표체여 IL-1β、IL-8、TNF-α적수평급의의。방법풍습성심장병환자38례여건강자37례(대조조)채용류식세포술검측외주혈림파세포상Notch-2、Notch-3、Notch-4화Jagged-1적표체수평,매련면역흡부법측정IL-1β、IL-8、TNF-α수평。결과 Notch-2、Notch-3、Notch-4/Jagged-1재풍습성심장병환자외주혈림파세포표체수평명현고우대조조,차이유통계학의의( P<0．05)。풍습성심장병환자외주혈IL-1β、IL-8、TNF-α수평고우대조조,차이유통계학의의(P<0．05),Notch-2、Notch-3、Notch-4/Jagged-1재풍습성심장병환자외주혈림파세포표체수평여풍습성심장병환자외주혈IL-1β、IL-8、TNF-α수평정정상관(P<0．05)。결론풍습성심장병환자Notch-2、Notch-3、Notch-4/Jagged-1재풍습성심장병환자외주혈림파세포상적표체,영향료IL-1β、IL-8、TNF-α수평,도치면역공능문란。
Objective To investigate the expression and correlation between Notch-2,3,4/Jagged-1 and IL-1β IL-8 and TNF-αin peripheral blood of rheumatic heart disease( RHD) patients. Methods Flow cytometry was used to analyze the propor-tion of the Notch-2,3,4/Jagged-1 in peripheral blood lymphocytes from 38 cases of rheumatic heart disease(RHD)patients and 37 cases of healthy people ( control group ) . Leavings serum of the same sample tested the centration of IL-1β IL-8 and TNF-α by ELISA. Results The levels of Notch-2,3,4/Jagged-1 in peripheral blood lymphocytes and IL-1βIL-8 and TNF-αof patients with RHD Were obviously significantly higher than the normal control(P<0. 05). And the levels of IL-1βIL-8 and TNF-αhad a positive relationship with Notch-2,3,4/Jagged-1 in peripheral blood lymphocytes of patients with RHD(P<0. 05). Conclusion The levels of IL-1β IL-8 and TNF-α probably can be affected by the change of Notch-2,3,4/Jagged-1 in peripheral blood lymphocytes of pa-tients with RHD.
