中国调味品
中國調味品
중국조미품
China Condiment
2015年
9期
94-97
,共4页
氨基酸%高效液相色谱%腐乳%条件优化
氨基痠%高效液相色譜%腐乳%條件優化
안기산%고효액상색보%부유%조건우화
amino acids%high performance liquid chromatography (HPLC)%fermented bean curd%optimization of conditions
氨基酸的种类及含量对腐乳后发酵过程的研究具有重要的理论和现实意义,所以必须建立一种便捷可靠的分析方法。高效液相色谱分析水平高、检测范围大,与衍生化技术联用后可以增加腐乳样品中氨基酸检测的灵敏度和精确度。对高效液相色谱法测定腐乳中氨基酸的方法进行了色谱条件优化,将其中对色谱峰分离及峰形有重要影响的梯度洗脱条件、柱温、流速进行优化,研究中以邻苯二甲醛和氯甲酸芴甲酯为衍生化试剂,优化后得到的色谱条件为:流动相A:磷酸氢二钠溶液(pH 7.8),流动相B:甲醇-乙腈-水溶液(体积比45∶45∶10),流速为0.6 mL/min,温度为50℃,检测波长为338,262 nm,进样体积为20μL,精确度及重现性中峰面积相对标准偏差 RSD 均小于0.2%,回收率在90.4%~118.7%,最终检测出腐乳后发酵过程中共有16种氨基酸。
氨基痠的種類及含量對腐乳後髮酵過程的研究具有重要的理論和現實意義,所以必鬚建立一種便捷可靠的分析方法。高效液相色譜分析水平高、檢測範圍大,與衍生化技術聯用後可以增加腐乳樣品中氨基痠檢測的靈敏度和精確度。對高效液相色譜法測定腐乳中氨基痠的方法進行瞭色譜條件優化,將其中對色譜峰分離及峰形有重要影響的梯度洗脫條件、柱溫、流速進行優化,研究中以鄰苯二甲醛和氯甲痠芴甲酯為衍生化試劑,優化後得到的色譜條件為:流動相A:燐痠氫二鈉溶液(pH 7.8),流動相B:甲醇-乙腈-水溶液(體積比45∶45∶10),流速為0.6 mL/min,溫度為50℃,檢測波長為338,262 nm,進樣體積為20μL,精確度及重現性中峰麵積相對標準偏差 RSD 均小于0.2%,迴收率在90.4%~118.7%,最終檢測齣腐乳後髮酵過程中共有16種氨基痠。
안기산적충류급함량대부유후발효과정적연구구유중요적이론화현실의의,소이필수건립일충편첩가고적분석방법。고효액상색보분석수평고、검측범위대,여연생화기술련용후가이증가부유양품중안기산검측적령민도화정학도。대고효액상색보법측정부유중안기산적방법진행료색보조건우화,장기중대색보봉분리급봉형유중요영향적제도세탈조건、주온、류속진행우화,연구중이린분이갑철화록갑산물갑지위연생화시제,우화후득도적색보조건위:류동상A:린산경이납용액(pH 7.8),류동상B:갑순-을정-수용액(체적비45∶45∶10),류속위0.6 mL/min,온도위50℃,검측파장위338,262 nm,진양체적위20μL,정학도급중현성중봉면적상대표준편차 RSD 균소우0.2%,회수솔재90.4%~118.7%,최종검측출부유후발효과정중공유16충안기산。
The kinds and content of amino acids have important theoretical and practical significance on the research of the post-fermentation of fermented bean curd, so it is necessary to establish a convenient and reliable analysis method.High performance liquid chromatography (HPLC)has high level of analysis and wide detection range,and can increase the sensitivity and accuracy of fermented bean curd samples after combination of derivatization technology.In this paper,the high performance liquid chromatography (HPLC)method is developed for the determination of amino acids in fermented bean curd to optimize the chromatographic conditions, which have important effects on the chromatographic peak separation and shape of gradient elution conditions,column temperature and flow rate,take formaldehyde and benzene chloride acid methyl fluorene as the derivatization reagent, the optimized chromatographic conditions are as follows:mobile phase A:disodium hydrogen phosphate solution (pH 7.8),mobile phase B:methanol,acetonitrile-water solution (volume ratio of 45∶45∶10),the flow rate of 0.6 mL/min,the temperature of 50 ℃,detection wavelength of 338, 262 nm,the sampling volume of 20μL, the relative standard deviation RSD of accuracy and reproducibility is less than 0.2%,the recovery rate of 90.4 %~118.7%,finally 16 kinds of amino acids are detected in the post-fermentation process of fermented bean curd.