CAJ | 학술논문

目的：探讨 microRNA －204（miR －204）在胃癌中的表达，研究 miR －204在胃癌细胞增殖中的作用和机制。方法：采用 RT －PCR 方法检测胃癌组织、胃癌细胞中的 miR －204相对表达量；分析 miR －204的表达与临床病理参数的关系；瞬时转染上调胃癌 SGC7901细胞中 miR －204的表达量，CCK8检测转染前后细胞增殖能力的变化；Western blot 检测转染前后 SGC7901细胞中 Bcl －2表达的变化。结果：胃癌组织中 miR －204相对表达量（1．94±0．78）明显低于正常胃组织（4．01±1．47）（P ＜0．01）；同样，人胃癌细胞株中 miR －204表达水平明显低于正常胃上皮细胞（P ＜0．01）。胃癌组织中 miR －204的表达水平与肿瘤的大小、远处转移和 TNM分期明显相关（P ＜0．05）。CCK8结果显示，miR －204 mimics 转染后 SCG7901细胞的增殖率较转染前明显下降（P ＜0．05），Western blot 结果显示胃癌细胞中 Bcl －2的表达水平较转染前明显下调（P＜0．05）。结论：miR －204在胃癌组织中低表达且与其恶性生物学行为相关，上调 miR －204可通过下调 Bcl－2的表达抑制胃癌细胞的增殖。
목적：탐토 microRNA －204（miR －204）재위암중적표체，연구 miR －204재위암세포증식중적작용화궤제。방법：채용 RT －PCR 방법검측위암조직、위암세포중적 miR －204상대표체량；분석 miR －204적표체여림상병리삼수적관계；순시전염상조위암 SGC7901세포중 miR －204적표체량，CCK8검측전염전후세포증식능력적변화；Western blot 검측전염전후 SGC7901세포중 Bcl －2표체적변화。결과：위암조직중 miR －204상대표체량（1．94±0．78）명현저우정상위조직（4．01±1．47）（P ＜0．01）；동양，인위암세포주중 miR －204표체수평명현저우정상위상피세포（P ＜0．01）。위암조직중 miR －204적표체수평여종류적대소、원처전이화 TNM분기명현상관（P ＜0．05）。CCK8결과현시，miR －204 mimics 전염후 SCG7901세포적증식솔교전염전명현하강（P ＜0．05），Western blot 결과현시위암세포중 Bcl －2적표체수평교전염전명현하조（P＜0．05）。결론：miR －204재위암조직중저표체차여기악성생물학행위상관，상조 miR －204가통과하조 Bcl－2적표체억제위암세포적증식。
Objective:To investigate the expression of miR -204 in patients with gastric cancer and to analyze the effect and mechanism of miR -204 on proliferation of gastric cancer.Methods:Gastric cancer tissues and gastric cancer cells were detected by RT -PCR.The relationships between the level of miR -204 in cancer tissues and the pathological characteristics were analyzed.miR -204 mimics was transiently transferred into SCG7901cells in vitro. Cell viability was analyzed by CCK8 assay.Expression of Bcl -2 protein was detected by Western blot.Results:The expression level of miR -204 was down -regulated in gastric cancer tissues as compared with the paired normal gas-tric tissues (P <0.05).The similar pattern was seen in gastric cancer cells.The expression of miR -204 in tumor tis-sues was associated with tumor size,lymphatic metastasis and TNMstages (P <0.05).CCK8 showed that over -ex-pression of miR -204 inhibited the proliferation of SGC7901 cells.Western blot indicated that over -expression of miR -204 led to a significant decrease in the Bcl -2 protein levels in SGC7901 cells.Conclusion:Low expression of miR -204 in gastric cancer have relation with malignant biological behavior.Up -regulation of miR -204 might in-hibit the proliferation of gastric cancer cells by down -regulation of Bcl -2.