组织工程与重建外科杂志
組織工程與重建外科雜誌
조직공정여중건외과잡지
Journal of Tissue Engineering and Reconstructive Surgery
2015年
4期
221-224
,共4页
张军%张耀明%王正辉%张青%罗花南%郑国玺%侯瑾%王波涛%杨叶叶%赵小燕%史艳霞
張軍%張耀明%王正輝%張青%囉花南%鄭國璽%侯瑾%王波濤%楊葉葉%趙小燕%史豔霞
장군%장요명%왕정휘%장청%라화남%정국새%후근%왕파도%양협협%조소연%사염하
脐血干细胞%分化%Sox9基因
臍血榦細胞%分化%Sox9基因
제혈간세포%분화%Sox9기인
Umbilical cord mesenchymal stem cells%Differentiation%SRY-related high mobility group-box gene 9
目的：探讨Sox9基因对脐血干细胞向软骨细胞分化的影响。方法体外分离培养人脐血干细胞，采用脂质体转染Sox9载体质粒，观察转染后细胞形态的变化，免疫组化染色观察collagenⅡ、aggrecan的表达，RT-PCR检测collagenⅠ、collagenⅡ、aggrecan的mRNA水平变化，Western blot检测collagenⅡ的表达变化。结果 Sox9对脐血干细胞形态无明显影响，与传统方法诱导组相比，Sox9转染后可明显促进脐血干细胞向软骨细胞分化。结论 Sox9对脐血干细胞的软骨分化有很强的调控作用，脐血干细胞可作为组织工程软骨一种良好的种子细胞。
目的：探討Sox9基因對臍血榦細胞嚮軟骨細胞分化的影響。方法體外分離培養人臍血榦細胞，採用脂質體轉染Sox9載體質粒，觀察轉染後細胞形態的變化，免疫組化染色觀察collagenⅡ、aggrecan的錶達，RT-PCR檢測collagenⅠ、collagenⅡ、aggrecan的mRNA水平變化，Western blot檢測collagenⅡ的錶達變化。結果 Sox9對臍血榦細胞形態無明顯影響，與傳統方法誘導組相比，Sox9轉染後可明顯促進臍血榦細胞嚮軟骨細胞分化。結論 Sox9對臍血榦細胞的軟骨分化有很彊的調控作用，臍血榦細胞可作為組織工程軟骨一種良好的種子細胞。
목적：탐토Sox9기인대제혈간세포향연골세포분화적영향。방법체외분리배양인제혈간세포，채용지질체전염Sox9재체질립，관찰전염후세포형태적변화，면역조화염색관찰collagenⅡ、aggrecan적표체，RT-PCR검측collagenⅠ、collagenⅡ、aggrecan적mRNA수평변화，Western blot검측collagenⅡ적표체변화。결과 Sox9대제혈간세포형태무명현영향，여전통방법유도조상비，Sox9전염후가명현촉진제혈간세포향연골세포분화。결론 Sox9대제혈간세포적연골분화유흔강적조공작용，제혈간세포가작위조직공정연골일충량호적충자세포。
Objective To investigate the effect of Sox9 gene on inducing the chondrogenic differentiation of human umbilical cord mesenchymal stem cells. Methods Human umbilical cord mesenchymal stem cells (hUCMSCs) were obtained by microdissection and digestion, and cultured in monolayer. The growth velocity and morphological changes of hUCMSCs were observed after Sox9 transfection. The expression of collagen Ⅱ and aggrecan were detected by immunohistochemistry. The mRNA expression of collagen Ⅰ, collagen Ⅱ and aggrecan were detected by RT-PCR, and collagen Ⅱ content was detected by western blot. Results The Sox9 gene had no significant effect on the morphology of hUCMSCs, and significantly accelerated the differentiation of hUCMSCs to chondrocytes compared with the traditional induction group. Conclusion Sox9 gene can regulate the differentiation of hUCMSCs, and hUCMSCs can be used as seed cells for cartilage tissue engineering.
