CAJ | 학술논문

检测并分析杰出有氧耐力表型与运动应激适应的表观遗传学基因调控模式特征的关联。方法：递增负荷法检测33名现役国家级耐力项目运动员和58名体育教育专业大学生（二级运动员）的最大摄氧量、个体乳酸阈，同步采用microarray及qRT‐PCR法检测训练诱导的c‐miRNA表达谱，分析杰出耐力表型与训练诱导的miRNA及去乙酰化酶（SIRT1）的基因表达调控特征的关联。结果：杰出耐力表型差异表达27条c‐miRNA ，18条上调，9条下调，其靶基因富集于AMPK、PPAR、MAPK及SIRT1信号通路，miRNA的调控作用使AM PK通路及下游通路活性上调，SIRT1的表达和活性水平显著上调并增强AM PK通路活性。结论：运动应激诱导的miRNA‐SIRT1的调控特征与杰出有氧耐力表型存在关联，对杰出耐力表型的形成具有促进作用，可作为预测有氧耐力发展潜力的表观遗传学分子标记应用于运动选材。
검측병분석걸출유양내력표형여운동응격괄응적표관유전학기인조공모식특정적관련。방법：체증부하법검측33명현역국가급내력항목운동원화58명체육교육전업대학생（이급운동원）적최대섭양량、개체유산역，동보채용microarray급qRT‐PCR법검측훈련유도적c‐miRNA표체보，분석걸출내력표형여훈련유도적miRNA급거을선화매（SIRT1）적기인표체조공특정적관련。결과：걸출내력표형차이표체27조c‐miRNA ，18조상조，9조하조，기파기인부집우AMPK、PPAR、MAPK급SIRT1신호통로，miRNA적조공작용사AM PK통로급하유통로활성상조，SIRT1적표체화활성수평현저상조병증강AM PK통로활성。결론：운동응격유도적miRNA‐SIRT1적조공특정여걸출유양내력표형존재관련，대걸출내력표형적형성구유촉진작용，가작위예측유양내력발전잠력적표관유전학분자표기응용우운동선재。
The objective of the study was to explore the relationship between the elite endurance phenotype and epigenetics gene regulating effect of miRNA‐SIRT1 in exercise stress adaption .33 active elite athletes (national grade) and 58 student athletes (grade 2) were engaged in this test .VO2max and ILT were measured .Differential expression profiles of c‐miR‐NAs induced by exercise were detected .Targetscan ,miRanda ,and miRBase were used to predict target genes of those miRNA ,and pathway analysis was performed for target gene by KEGG and IPA .Result :There were 27 c‐miRNAs which regulated the pathway of AMPK ,PPAR ,MAPK ,SIRT1 ,and their regulating characters were validated to promote the formation of the elite endurance .It could be concluded that there was relationship between miRNA‐SIRT1 and exercise stress adaption ,the profile of c‐miRNAs induced by exercise could be used as an epigenetic biomarker on forecasting and valuing the potential of aerobic endurance .