检验医学与临床
檢驗醫學與臨床
검험의학여림상
Laboratory Medicine and Clinic
2015年
17期
2533-2534
,共2页
抗凝蛋白%抗凝血酶Ⅲ%蛋白C%蛋白S%温度%时间
抗凝蛋白%抗凝血酶Ⅲ%蛋白C%蛋白S%溫度%時間
항응단백%항응혈매Ⅲ%단백C%단백S%온도%시간
anticoagulation protein%antithrombinⅢ%protein C%protein S%temperature%time
目的:探讨标本存放温度和时间对血浆中抗凝蛋白活性的影响。方法用真空采血管采集住院患者空腹静脉血20例,无黄疸、脂血、溶血,即刻以3000r/min离心10min,上机测得的结果与在室温(20~25℃)、4℃和-20℃放置4h、12h、24h、7d、14d的血浆标本测得的结果分别进行比较。结果在室温下保存ATⅢ、PC活性24h内均无明显变化,仅PS活性明显降低,7d和14d时检测抗凝蛋白活性均明显降低(P<0.05);在4℃放置7dATⅢ、PC活性有所降低,但与即时活性相比差异无统计学意义,而PS活性明显下降(P<0.05),14d时三者活性均明显下降(P<0.05);在-20℃保存14d甚至更长时间抗凝蛋白活性均无明显降低。结论为保证抗凝蛋白活性,血液标本应置于低温保存并尽快检测。
目的:探討標本存放溫度和時間對血漿中抗凝蛋白活性的影響。方法用真空採血管採集住院患者空腹靜脈血20例,無黃疸、脂血、溶血,即刻以3000r/min離心10min,上機測得的結果與在室溫(20~25℃)、4℃和-20℃放置4h、12h、24h、7d、14d的血漿標本測得的結果分彆進行比較。結果在室溫下保存ATⅢ、PC活性24h內均無明顯變化,僅PS活性明顯降低,7d和14d時檢測抗凝蛋白活性均明顯降低(P<0.05);在4℃放置7dATⅢ、PC活性有所降低,但與即時活性相比差異無統計學意義,而PS活性明顯下降(P<0.05),14d時三者活性均明顯下降(P<0.05);在-20℃保存14d甚至更長時間抗凝蛋白活性均無明顯降低。結論為保證抗凝蛋白活性,血液標本應置于低溫保存併儘快檢測。
목적:탐토표본존방온도화시간대혈장중항응단백활성적영향。방법용진공채혈관채집주원환자공복정맥혈20례,무황달、지혈、용혈,즉각이3000r/min리심10min,상궤측득적결과여재실온(20~25℃)、4℃화-20℃방치4h、12h、24h、7d、14d적혈장표본측득적결과분별진행비교。결과재실온하보존ATⅢ、PC활성24h내균무명현변화,부PS활성명현강저,7d화14d시검측항응단백활성균명현강저(P<0.05);재4℃방치7dATⅢ、PC활성유소강저,단여즉시활성상비차이무통계학의의,이PS활성명현하강(P<0.05),14d시삼자활성균명현하강(P<0.05);재-20℃보존14d심지경장시간항응단백활성균무명현강저。결론위보증항응단백활성,혈액표본응치우저온보존병진쾌검측。
Objective To investigate the influence of storage time and temperature on anticoagulation protein activity in plasma .Methods Fasting venous blood was collected from 20 inpatients without jaundice ,lipidemia and hemolysis by vacutainer ,immediately centrifuged by 3 000 r/min for 10 min and detected .The detected results were compared with those stored in the room temperature(20-25 ℃) ,at 4 ℃ and -20 ℃ ,for 4 h ,12 h ,24 h ,7 d ,14 d respectively .Results The ATⅢ and PC activity of the sample stored in the room temperature within 24 h had no significant change ,but only the PS activity was significantly decreased ,the anticoagulation protein activity detected on 7 ,14 d was significantly decreased(P<0 .05);the ATⅢ and PC activity of the sample stored at 4 ℃ for 7 d was de‐creased ,but the difference had no statistical significance compared with those of immediately detected samples ,but the PS activity was significantly decreased(P< 0 .05) ,the activity of 3 indexes on 14 d was decreased significantly (P<0 .05);the activity of anticoagulation protein of the sample stored at -20 ℃ for 14 d or even longer time had no obvious decrease .Conclusion The blood samples should be placed in the low temperature and detected as soon as possible for ensuring the anticoagulant protein activity .