CAJ | 학술논문

目的：纯化和体外培养子痫前期患者的胎盘间充质干细胞（ pMSCs），研究子痫前期患者pMSCs的免疫抑制能力。方法从子痫前期患者及正常妊娠者胎盘组织中纯化培养pMSCs，应用Transwell板将pMSCs与混合淋巴细胞共培养，检测淋巴细胞数，计算淋巴细胞增殖抑制率。在培养基中加入干扰素γ，分别从mRNA水平和蛋白质水平检测pMSCs的吲哚胺双加氧酶（indoleamine 2，3-dioxygenase，IDO）表达水平。结果来源于子痫前期患者的PMSCs和正常妊娠者的形态相同，表达CD44、CD29、CD73、CD90、CD105、HLA-I类分子，极少表达CD34、CD45、CD14及HLA-Ⅱ分子。能够在体外被诱导分化为成骨细胞及成脂细胞。细胞释放的可溶性分子具有抑制淋巴细胞增殖的作用；细胞表达低水平的IDO，但当受到IFN-γ刺激后，表达水平显著提高（ P ＜0．05）。对照正常妊娠组和子痫前期组pMSCs对淋巴细胞增殖的抑制率和表达IDO水平，差异均不具有显著性（ P ＞0．05）。结论 pMSCs所表达的IDO和释放的可溶性分子可能与PE的发生、发展无关；由于现有体外培养技术不能反映pMSCs在体内的状态，也可能导致原本存在的差异难以体现出来。
목적：순화화체외배양자간전기환자적태반간충질간세포（ pMSCs），연구자간전기환자pMSCs적면역억제능력。방법종자간전기환자급정상임신자태반조직중순화배양pMSCs，응용Transwell판장pMSCs여혼합림파세포공배양，검측림파세포수，계산림파세포증식억제솔。재배양기중가입간우소γ，분별종mRNA수평화단백질수평검측pMSCs적신타알쌍가양매（indoleamine 2，3-dioxygenase，IDO）표체수평。결과래원우자간전기환자적PMSCs화정상임신자적형태상동，표체CD44、CD29、CD73、CD90、CD105、HLA-I류분자，겁소표체CD34、CD45、CD14급HLA-Ⅱ분자。능구재체외피유도분화위성골세포급성지세포。세포석방적가용성분자구유억제림파세포증식적작용；세포표체저수평적IDO，단당수도IFN-γ자격후，표체수평현저제고（ P ＜0．05）。대조정상임신조화자간전기조pMSCs대림파세포증식적억제솔화표체IDO수평，차이균불구유현저성（ P ＞0．05）。결론 pMSCs소표체적IDO화석방적가용성분자가능여PE적발생、발전무관；유우현유체외배양기술불능반영pMSCs재체내적상태，야가능도치원본존재적차이난이체현출래。
Objective To isolate and culture placental mesenchymal stem cells (pMSCs)from women with pre-eclampsia and study the immunosuppressive effects of these cells.Methods Placental mesenchymal stem cells isolated from placenta of women with pre-eclampsia and normal pregnancy were cultured in vitro.Co-culture of placental mesenchymal stem cells and mixed lymphocyte were performed on Transwell and the lymphocytes were counted and the inhibiting ratio of lymphocytes proliferation was calculated.Moreo-ver, placental mesenchymal stem cells was treated with interferonγ(IFN-γ).The expression of Indoleamine 2,3-Dioxygenase (IDO) mRNA and protein of these cells were determined by qRT-PCR and ELISA.Results Placental mesenchymal stem cells from women with preelampsia had the same morphology with normal pregnancy.Most of these cells were positive for CD44,CD29,CD73,CD90, CD105,HLA-I molecules but negative for CD34,CD45,CD14, HLA-Ⅱmolecules.They could also be induced to adipogenic and os-teogenic differentiation.Soluble factors secreted by these cells may suppress the proliferation of lymphocytes.IFN-γtreated cells pro-voked significantly more IDO production than the untreated ( P <0.05).No significant differences were seen between the cells from normal pregnancy and PE, both on suppression of lymphocytes proliferation, as well as expression of IDO, regardless of the IFN-γstimulation.Conclusion The expression of IDO and soluble factors by placental mesenchymal stem cells might have no association with the pre-eclampsia pathogenisis.Since in vitro culture conditions can not reflect the actual features of placental mesenchymal stem cells, the difference between normal and pre-eclampsia may not be revealed.