CAJ | 학술논문

目的：测定不同启动子的重组干扰素（IFN）慢病毒（LV）共感染对5型腺病毒在体外293A细胞中复制的影响。方法以重组LV-EF1α-IFNγ、LV-CMV-IFNα、LV-CMV-IFNγ、LV-Ubi-IFNα与5型腺病毒共感染293A细胞，72 h后，荧光定量PCR法测定5型腺病毒载量。结果 LV-EF1α-IFNγ、LV-CMV-IFNα、LV-CMV-IFNγ、LV-Ubi-IFNα共感染组的腺病毒载量低于非共感染对照组（ P ＜0．01）。结论使用重组LV-IFN感染293A后，能够显著抑制腺病毒复制，为进一步开展HIV／AIDS等病毒性疾病的基因治疗研究提供参考。
목적：측정불동계동자적중조간우소（IFN）만병독（LV）공감염대5형선병독재체외293A세포중복제적영향。방법이중조LV-EF1α-IFNγ、LV-CMV-IFNα、LV-CMV-IFNγ、LV-Ubi-IFNα여5형선병독공감염293A세포，72 h후，형광정량PCR법측정5형선병독재량。결과 LV-EF1α-IFNγ、LV-CMV-IFNα、LV-CMV-IFNγ、LV-Ubi-IFNα공감염조적선병독재량저우비공감염대조조（ P ＜0．01）。결론사용중조LV-IFN감염293A후，능구현저억제선병독복제，위진일보개전HIV／AIDS등병독성질병적기인치료연구제공삼고。
Objective To determine the inhibition effect of recombinant interferon lentivirus co-infection with adenovirus 5 a-mong 293A cells in vitro .Methods Recombinant LV-EF1α-IFNγ, LV-CMV-IFNα,LV-CMV-IFNγ, LV-Ubi-IFNαand adenovirus 5 co-infection among 293A cells were used and 72 hours later, fluorescence quantitative PCR method was adopted to detect adenovirus 5 viral loads.Resulst Viral loads of LV-EF1α-IFNγ, LV-CMV-IFNα, LV-CMV-IFNγ, LV-Ubi-IFNαco-infection group were signifi-cantly lower than those of control group( P <0.01).Conclusion LV-IFN co-infection can inhibit adenovirus replication among 293A cells, which lays the foundation for the gene therapy of viral diseases such as HIV/AIDS.