实用癌症杂志
實用癌癥雜誌
실용암증잡지
The Practical Journal of Cancer
2015年
8期
1135-1138
,共4页
宫颈癌%阴道内环境%菌群
宮頸癌%陰道內環境%菌群
궁경암%음도내배경%균군
Cervical cancer%Vaginal environment%Flora
目的 比较正常人群与宫颈癌人群阴道内环境及常见菌群的差异性,为预防和诊断宫颈癌提供一种新型的标志物或手段. 方法 采用病例对照研究方法进行配对样本量设计,选取经过病理检查确诊的50名宫颈癌患者,按照年龄、文化程度、收入水平、婚姻及性生活情况进行配对选取50名健康体检者,共同采集阴道内分泌物样本,利用五联检试剂及非厌氧的菌群培养方式检测阴道内环境及阴道内常见菌群,数据分析采用t检验或卡方检验. 结果 宫颈癌组的PH值为(4.8 ±0.03),明显高于对照组的(4.0 ±0.03). 乳酸杆菌、霉菌、滴虫在宫颈癌患者中的检出率分别为88%、74%和42%,与对照组比较都存在统计学差异;但在革兰阴性菌和革兰阳性菌的对比上无明显差异性. 在宫颈癌患者和健康人群中都可检出大肠埃希菌、产酸克雷伯菌、表皮葡萄球菌、粪肠球菌和尿肠球菌,但宫颈癌患者中检出的特殊类型的菌种有奇异变形杆菌、阴沟肠杆菌和易变微球菌等. 宫颈癌患者革兰氏阴性菌需氧菌群多样性指数低于对照组,但革兰氏阳性菌需氧菌群多样性却高于对照组. 结论 宫颈癌患者阴道PH值较正常人群明显升高,且不同微生物的检出比例和菌群多样性与正常人群存在明显不同.
目的 比較正常人群與宮頸癌人群陰道內環境及常見菌群的差異性,為預防和診斷宮頸癌提供一種新型的標誌物或手段. 方法 採用病例對照研究方法進行配對樣本量設計,選取經過病理檢查確診的50名宮頸癌患者,按照年齡、文化程度、收入水平、婚姻及性生活情況進行配對選取50名健康體檢者,共同採集陰道內分泌物樣本,利用五聯檢試劑及非厭氧的菌群培養方式檢測陰道內環境及陰道內常見菌群,數據分析採用t檢驗或卡方檢驗. 結果 宮頸癌組的PH值為(4.8 ±0.03),明顯高于對照組的(4.0 ±0.03). 乳痠桿菌、黴菌、滴蟲在宮頸癌患者中的檢齣率分彆為88%、74%和42%,與對照組比較都存在統計學差異;但在革蘭陰性菌和革蘭暘性菌的對比上無明顯差異性. 在宮頸癌患者和健康人群中都可檢齣大腸埃希菌、產痠剋雷伯菌、錶皮葡萄毬菌、糞腸毬菌和尿腸毬菌,但宮頸癌患者中檢齣的特殊類型的菌種有奇異變形桿菌、陰溝腸桿菌和易變微毬菌等. 宮頸癌患者革蘭氏陰性菌需氧菌群多樣性指數低于對照組,但革蘭氏暘性菌需氧菌群多樣性卻高于對照組. 結論 宮頸癌患者陰道PH值較正常人群明顯升高,且不同微生物的檢齣比例和菌群多樣性與正常人群存在明顯不同.
목적 비교정상인군여궁경암인군음도내배경급상견균군적차이성,위예방화진단궁경암제공일충신형적표지물혹수단. 방법 채용병례대조연구방법진행배대양본량설계,선취경과병리검사학진적50명궁경암환자,안조년령、문화정도、수입수평、혼인급성생활정황진행배대선취50명건강체검자,공동채집음도내분비물양본,이용오련검시제급비염양적균군배양방식검측음도내배경급음도내상견균군,수거분석채용t검험혹잡방검험. 결과 궁경암조적PH치위(4.8 ±0.03),명현고우대조조적(4.0 ±0.03). 유산간균、매균、적충재궁경암환자중적검출솔분별위88%、74%화42%,여대조조비교도존재통계학차이;단재혁란음성균화혁란양성균적대비상무명현차이성. 재궁경암환자화건강인군중도가검출대장애희균、산산극뢰백균、표피포도구균、분장구균화뇨장구균,단궁경암환자중검출적특수류형적균충유기이변형간균、음구장간균화역변미구균등. 궁경암환자혁란씨음성균수양균군다양성지수저우대조조,단혁란씨양성균수양균군다양성각고우대조조. 결론 궁경암환자음도PH치교정상인군명현승고,차불동미생물적검출비례화균군다양성여정상인군존재명현불동.
Objective To compare the differences of vaginal environment and common flora between cervical cancer pa-tients and healthy patients,and provide a new indicator or method in preventing and curing cervical cancer.Methods A case-control study was conducted in all 50 cervical cancer patients and 50 paired healthy patients.The paired criteria were age,degree of education,income,marriage and sexual life.The vaginal environment and common flora were detected and analyzed by 5 inspec-tion reagents and non-anaerobic bacteria culture.T test and χ2 test were used to compare the differences between the 2 groups.Re-sults PH value(4.8 ±0.03) in cervical cancer patients was higher than that(4.0 ±0.03) in healthy patients.The detection rate of lactic acid bacillus,mould and trichomonad were 88%,74%,42% respectively,which showed significant differences be-tween the 2 groups.However,there were no differences in gram-positive aerobic bacteria and gram-negative aerobic bacteria be-tween the 2 groups.The common floras in both groups were E.coli,klebsiella oxytoca,staphylococcus epidermidis,enterococcus faecalis and enterococcus faecium.But some unique types of bacteria were also found in cervical cancer patients,such as bacillus milabilis,enterobacter cloacae,micrococcus varians et al.The Shannon index of gram-negative bacteria in cervical cancer patients was lower than that of the control group,but showed higher values in Shannon index of gram-positive bacteria.Conclusion Vagi-nal PH value in cervical cancer patients is much higher than the healthy patients.There has significant difference in vaginal envi-ronment and diversity of bacteria between cervical cancer patients and healthy patients.
