实用癌症杂志
實用癌癥雜誌
실용암증잡지
The Practical Journal of Cancer
2015年
8期
1116-1118
,共3页
血浆%EBV-DNA%鼻咽癌分期
血漿%EBV-DNA%鼻嚥癌分期
혈장%EBV-DNA%비인암분기
Plasma%EBV-DNA%Staging of nasopharyngeal carcinoma( NPC)
目的 研究血浆EBV-DNA表达水平与鼻咽癌分期的相关性. 方法 选择了55例鼻咽癌患者,同时选取同期住院治疗的55例其他类型的肿瘤患者作为其他类肿瘤对照组及55例体检健康者为健康对照组,比较分析鼻咽癌组与对照组患者的血浆EBV-DNA阳性检出情况,鼻咽癌组患者与对照组血浆EBV-DNA浓度及血浆EBV-DNA阳性的鼻咽患者早期和晚期的拷贝数. 结果 鼻咽癌组血浆EBV-DNA检出阳性率为72.7%,明显高于其他类肿瘤对照组的阳性率(23.7%)以及健康对照组(10.9%),具有统计学的意义(P<0.05). 鼻咽癌组患者的血浆EBV-DNA浓度较高,达到了13654.5 copies/mL,而其他类肿瘤对照组及健康对照组的血浆EBV-DNA浓度均为0,具有统计学的意义( P<0.05). 观察还发现血浆EBV-DNA阳性的鼻咽患者晚期血浆EBV-DNA拷贝数较高,达到了(201.48 ±176.19),明显高于早期的血浆EBV-DNA拷贝数(9.60 ±10.77),两组数据进行比较,具有统计学的意义(t=4.3312,P=0.0001). 结论 荧光定量的PCR检测血浆EBV-DNA是1种比较可靠的方法,能够有效诊断鼻咽癌,且血浆EBV-DNA与鼻咽癌的分期关系密切,该方法适合广泛地推广.
目的 研究血漿EBV-DNA錶達水平與鼻嚥癌分期的相關性. 方法 選擇瞭55例鼻嚥癌患者,同時選取同期住院治療的55例其他類型的腫瘤患者作為其他類腫瘤對照組及55例體檢健康者為健康對照組,比較分析鼻嚥癌組與對照組患者的血漿EBV-DNA暘性檢齣情況,鼻嚥癌組患者與對照組血漿EBV-DNA濃度及血漿EBV-DNA暘性的鼻嚥患者早期和晚期的拷貝數. 結果 鼻嚥癌組血漿EBV-DNA檢齣暘性率為72.7%,明顯高于其他類腫瘤對照組的暘性率(23.7%)以及健康對照組(10.9%),具有統計學的意義(P<0.05). 鼻嚥癌組患者的血漿EBV-DNA濃度較高,達到瞭13654.5 copies/mL,而其他類腫瘤對照組及健康對照組的血漿EBV-DNA濃度均為0,具有統計學的意義( P<0.05). 觀察還髮現血漿EBV-DNA暘性的鼻嚥患者晚期血漿EBV-DNA拷貝數較高,達到瞭(201.48 ±176.19),明顯高于早期的血漿EBV-DNA拷貝數(9.60 ±10.77),兩組數據進行比較,具有統計學的意義(t=4.3312,P=0.0001). 結論 熒光定量的PCR檢測血漿EBV-DNA是1種比較可靠的方法,能夠有效診斷鼻嚥癌,且血漿EBV-DNA與鼻嚥癌的分期關繫密切,該方法適閤廣汎地推廣.
목적 연구혈장EBV-DNA표체수평여비인암분기적상관성. 방법 선택료55례비인암환자,동시선취동기주원치료적55례기타류형적종류환자작위기타류종류대조조급55례체검건강자위건강대조조,비교분석비인암조여대조조환자적혈장EBV-DNA양성검출정황,비인암조환자여대조조혈장EBV-DNA농도급혈장EBV-DNA양성적비인환자조기화만기적고패수. 결과 비인암조혈장EBV-DNA검출양성솔위72.7%,명현고우기타류종류대조조적양성솔(23.7%)이급건강대조조(10.9%),구유통계학적의의(P<0.05). 비인암조환자적혈장EBV-DNA농도교고,체도료13654.5 copies/mL,이기타류종류대조조급건강대조조적혈장EBV-DNA농도균위0,구유통계학적의의( P<0.05). 관찰환발현혈장EBV-DNA양성적비인환자만기혈장EBV-DNA고패수교고,체도료(201.48 ±176.19),명현고우조기적혈장EBV-DNA고패수(9.60 ±10.77),량조수거진행비교,구유통계학적의의(t=4.3312,P=0.0001). 결론 형광정량적PCR검측혈장EBV-DNA시1충비교가고적방법,능구유효진단비인암,차혈장EBV-DNA여비인암적분기관계밀절,해방법괄합엄범지추엄.
Objective To research the correlation between the expression level of plasma EBV-DNA and nasopharyngeal carcinoma( NPC) staging.Methods 55 cases of NPC,55 cases of other types of cancer as tumor control group and 55 cases of healthy patients as healthy control group were selected,all patients were treated in our hospital at the same period.The positive rates and concentration of plasma EBV-DNA of the 3 groups,the plasma EBV-DNA copy number at early and last period of plas-ma EBV-DNA positive patients were compared and analyzed.Results The positive rate of NPC group was 72.7%,which was significantly higher than of other types of tumors (23.7%) and healthy control group(10.9%),there had statistical difference(P<0.05).The concentrations of plasma EBV-DNA in NPC patients reached13654.5 copies/mL,while that of other groups both were 0 copies/mL,there had statistical difference(P<0.05).Among the plasma EBV-DNA positive patients,the copies of plasma EBV-DNA at last period reached (201.48 ±176.19),which was higher than at early period(9.60 ±10.77),there had statistical difference(t=4.3312,P=0.0001).Conclusion Fluorescence quantitative PCR detection of plasma EBV-DNA is reliable,it can effectively diagnose NPC,and plasma EBV-DNA is closely related with NPC staging.It is worthy of promotion.
