东南大学学报（医学版）
東南大學學報（醫學版）
동남대학학보（의학판）
Journal of Southeast University (Medical Science Edition)
2015年
5期
679-683
,共5页
刘培党%金海振%赵静%唐萌
劉培黨%金海振%趙靜%唐萌
류배당%금해진%조정%당맹
纳米银%胶质瘤%电离辐射%细胞凋亡%小胶质细胞
納米銀%膠質瘤%電離輻射%細胞凋亡%小膠質細胞
납미은%효질류%전리복사%세포조망%소효질세포
nanosilver%glioma%ionizing radiation%apoptosis%microglia
目的：研究纳米银联合辐射后大鼠脑胶质瘤细胞凋亡与瘤内小胶质细胞的变化及其关系。方法：将C6细胞立体定向接种于大鼠右侧尾状核，建模后第8天将荷瘤鼠随机分成辐射对照组和纳米银联合辐射组，分别立体定向注入等体积的去离子水及20μg的纳米银，并行单次电离辐射。应用原位末端转移酶标记（ TUNEL）法和OX42免疫组织化学染色分别检测辐射后瘤组织内细胞凋亡及小胶质细胞活性的动态变化。结果：TUNEL阳性细胞数目在辐射后6 h显著增加，24 h明显减少；辐射后6 h胶质瘤内小胶质细胞开始激活，随着时间的延长活性进一步增强。辐射后6 h 和24 h，纳米银联合辐射组TUNEL阳性细胞数目和小胶质细胞活性均较辐射对照组增加，差异有统计学意义（ P＜0.001）。小胶质细胞活化与胶质瘤细胞凋亡的变化趋势不同，前者迟于后者。结论：纳米银联合辐射后胶质瘤细胞凋亡增加，小胶质细胞活化；激活的小胶质细胞可能没有参与胶质瘤细胞的急性凋亡过程。
目的：研究納米銀聯閤輻射後大鼠腦膠質瘤細胞凋亡與瘤內小膠質細胞的變化及其關繫。方法：將C6細胞立體定嚮接種于大鼠右側尾狀覈，建模後第8天將荷瘤鼠隨機分成輻射對照組和納米銀聯閤輻射組，分彆立體定嚮註入等體積的去離子水及20μg的納米銀，併行單次電離輻射。應用原位末耑轉移酶標記（ TUNEL）法和OX42免疫組織化學染色分彆檢測輻射後瘤組織內細胞凋亡及小膠質細胞活性的動態變化。結果：TUNEL暘性細胞數目在輻射後6 h顯著增加，24 h明顯減少；輻射後6 h膠質瘤內小膠質細胞開始激活，隨著時間的延長活性進一步增彊。輻射後6 h 和24 h，納米銀聯閤輻射組TUNEL暘性細胞數目和小膠質細胞活性均較輻射對照組增加，差異有統計學意義（ P＜0.001）。小膠質細胞活化與膠質瘤細胞凋亡的變化趨勢不同，前者遲于後者。結論：納米銀聯閤輻射後膠質瘤細胞凋亡增加，小膠質細胞活化；激活的小膠質細胞可能沒有參與膠質瘤細胞的急性凋亡過程。
목적：연구납미은연합복사후대서뇌효질류세포조망여류내소효질세포적변화급기관계。방법：장C6세포입체정향접충우대서우측미상핵，건모후제8천장하류서수궤분성복사대조조화납미은연합복사조，분별입체정향주입등체적적거리자수급20μg적납미은，병행단차전리복사。응용원위말단전이매표기（ TUNEL）법화OX42면역조직화학염색분별검측복사후류조직내세포조망급소효질세포활성적동태변화。결과：TUNEL양성세포수목재복사후6 h현저증가，24 h명현감소；복사후6 h효질류내소효질세포개시격활，수착시간적연장활성진일보증강。복사후6 h 화24 h，납미은연합복사조TUNEL양성세포수목화소효질세포활성균교복사대조조증가，차이유통계학의의（ P＜0.001）。소효질세포활화여효질류세포조망적변화추세불동，전자지우후자。결론：납미은연합복사후효질류세포조망증가，소효질세포활화；격활적소효질세포가능몰유삼여효질류세포적급성조망과정。
Objective: To study the changes in apoptosis of glioma cells and microglia activity and their correlation following nanosilver plus radiation.Methods:C6 glioma cells were stereotactically transplanted into the rat'right caudate nucleus.8 days after inoculation, the tumor-bearing animals were randomly divided into the irradiated control group and combined treatment group, which then received intratumoral injections of deionized water or nanosilver of the same volume ( 20 μg ) , followed by a single dose of ionizing radiation.The dynamic changes of apoptosis and microglia activity in the tumor tissue were examined by TUNEL assay and OX42 immunohistochemical staining, respectively.Results:The number of TUNEL-positive cells increased significantly 6 h after irradiation, and then dropped significantly 24 h postirradiation.Microglia activity was expressed 6 h after the radiation, and it grew further over time.Furthermore, compared with that in the irradiated control group, the number of TUNEL-positive cells and microglia activity increased significantly in the combined treatment group 6 h and 24 h after the radiation(P<0.001).The expression trends of microglia activity and TUNEL-positive cells were different, with the former being later.Conclusion:The combination of nanosilver with radiation can promote glioma cells apoptosis and activate microglia.The activated microglia may not participate in the acute process of glioma cell apoptosis.
