CAJ | 학술논문

目的：研究溶瘤性腺病毒介导的 canstatin 基因表达对 A549细胞的抑制作用。方法：将构建的溶瘤性腺病毒载体 CRAd －canstatin 感染 A549细胞。Western blot 方法鉴定 canstatin 基因在 A549细胞中的表达；结晶紫染色法和 MTT 法检测重组病毒体外对细胞的生长抑制作用；将腺病毒注射入荷瘤的裸鼠体内，通过测量肿瘤体积，形态学观察等方法验证 CRAd －canstatin 对肿瘤的抑制效果。结果：体外结果显示腺病毒介导的canstatin 基因在 A549细胞中有效表达。体内结果显示相比对照组而言，肿瘤体积明显缩小。结论：初步的实验表明溶瘤性腺病毒介导的 CRAd －canstatin 基因有望用于肺癌细胞的治疗。
목적：연구용류성선병독개도적 canstatin 기인표체대 A549세포적억제작용。방법：장구건적용류성선병독재체 CRAd －canstatin 감염 A549세포。Western blot 방법감정 canstatin 기인재 A549세포중적표체；결정자염색법화 MTT 법검측중조병독체외대세포적생장억제작용；장선병독주사입하류적라서체내，통과측량종류체적，형태학관찰등방법험증 CRAd －canstatin 대종류적억제효과。결과：체외결과현시선병독개도적canstatin 기인재 A549세포중유효표체。체내결과현시상비대조조이언，종류체적명현축소。결론：초보적실험표명용류성선병독개도적 CRAd －canstatin 기인유망용우폐암세포적치료。
Objective:To investigate the anti -cancer effect by CRAd -canstatin vector no A549 lung cancer cells.Methods:The oncolytic adenovirus CRAd -canstatin vector was successfully reconstructed and canstatin protein was detected by Western blot after 24h infection to A549 lung cancer cells.Results:The in vivo study showed that the volumes of tumor were greatly reduced compare with control groups.Conclusion:Experimently oncolytic adenovirus CRAd -canstatin could be effectively applied to the treatment of lung cancer gene therapy.