世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
World Journal of Integrated Traditional and Western Medicine
2015年
8期
1079-1082
,共4页
蔡清宇%唐慧慧%卢婧%路森森
蔡清宇%唐慧慧%盧婧%路森森
채청우%당혜혜%로청%로삼삼
羊蹄总皂苷%四氯化碳%肝损伤
羊蹄總皂苷%四氯化碳%肝損傷
양제총조감%사록화탄%간손상
Rumex Japonicus Total Saponins%Carbon Tetrachloride%Liver Injury
目的:研究羊蹄总皂苷(BJS)对小鼠肝损伤的保护作用及其机制。方法将健康小鼠随机分为空白对照组、模型组、联苯双酯组、BJS 低中高剂量组(100、200、400 mg/ kg),连续给药7 d;末次给药2 h 后,腹腔注射0.1%的 CCl4橄榄油溶液10 ml/ kg,建立小鼠急性肝损伤模型;观察 BJS 对急性肝损伤小鼠肝脏指数、组织病理学改变的影响;采用 ELISA 法检测小鼠血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)以及肝组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽(GSH)、白介素6(IL -6)和肿瘤坏死因子-α(TNF -α)含量的变化。结果与模型组相比,BJS治疗组肝脏指数明显降低,肝组织病理学改变明显改善;BJS 能显著降低血清 ALT 和 AST 含量(P ﹤0.05,P ﹤0.01),降低肝组织中 MDA、IL -6及 TNF -α含量(P ﹤0.05,P ﹤0.01),上调 GSH 和 SOD活性(P ﹤0.05,P ﹤0.01)。结论 BJS 对 CCl4引起的小鼠肝脏损伤有保护作用,其保护机制与减少自由基产生,提高抗氧活酶活性以及降低炎症细胞因子 TNF -α和 IL -6有关。
目的:研究羊蹄總皂苷(BJS)對小鼠肝損傷的保護作用及其機製。方法將健康小鼠隨機分為空白對照組、模型組、聯苯雙酯組、BJS 低中高劑量組(100、200、400 mg/ kg),連續給藥7 d;末次給藥2 h 後,腹腔註射0.1%的 CCl4橄欖油溶液10 ml/ kg,建立小鼠急性肝損傷模型;觀察 BJS 對急性肝損傷小鼠肝髒指數、組織病理學改變的影響;採用 ELISA 法檢測小鼠血清中丙氨痠氨基轉移酶(ALT)和天門鼕氨痠氨基轉移酶(AST)以及肝組織中超氧化物歧化酶(SOD)、丙二醛(MDA)、穀胱甘肽(GSH)、白介素6(IL -6)和腫瘤壞死因子-α(TNF -α)含量的變化。結果與模型組相比,BJS治療組肝髒指數明顯降低,肝組織病理學改變明顯改善;BJS 能顯著降低血清 ALT 和 AST 含量(P ﹤0.05,P ﹤0.01),降低肝組織中 MDA、IL -6及 TNF -α含量(P ﹤0.05,P ﹤0.01),上調 GSH 和 SOD活性(P ﹤0.05,P ﹤0.01)。結論 BJS 對 CCl4引起的小鼠肝髒損傷有保護作用,其保護機製與減少自由基產生,提高抗氧活酶活性以及降低炎癥細胞因子 TNF -α和 IL -6有關。
목적:연구양제총조감(BJS)대소서간손상적보호작용급기궤제。방법장건강소서수궤분위공백대조조、모형조、련분쌍지조、BJS 저중고제량조(100、200、400 mg/ kg),련속급약7 d;말차급약2 h 후,복강주사0.1%적 CCl4감람유용액10 ml/ kg,건립소서급성간손상모형;관찰 BJS 대급성간손상소서간장지수、조직병이학개변적영향;채용 ELISA 법검측소서혈청중병안산안기전이매(ALT)화천문동안산안기전이매(AST)이급간조직중초양화물기화매(SOD)、병이철(MDA)、곡광감태(GSH)、백개소6(IL -6)화종류배사인자-α(TNF -α)함량적변화。결과여모형조상비,BJS치료조간장지수명현강저,간조직병이학개변명현개선;BJS 능현저강저혈청 ALT 화 AST 함량(P ﹤0.05,P ﹤0.01),강저간조직중 MDA、IL -6급 TNF -α함량(P ﹤0.05,P ﹤0.01),상조 GSH 화 SOD활성(P ﹤0.05,P ﹤0.01)。결론 BJS 대 CCl4인기적소서간장손상유보호작용,기보호궤제여감소자유기산생,제고항양활매활성이급강저염증세포인자 TNF -α화 IL -6유관。
Objective To study the protective effect of rumex japonicus total saponins(RJTS)on liver injury in mice and its mechanism. Methods The healthy mice were randomized into a blank control group,a model group,a bifendate group,and RJTS low - dose,middle - dose and high - dose groups(100 mg/ kg,200 mg/ kg,400 mg/ kg). The medicine was given successively for 7 days. Two hours after the last medication,0. 1% CCl4 olive oil solution 10ml/ kg was injected intraperitoneally to establish the model of a-cute liver injury in mice. The impacts of RJTS on liver indexes and histopathological changes were observed in the mice of acute liver injury. ELISA was used to determine the levels of ALT and AST in serum,SOD,MDA, GSH,IL - 6 and TNF - α in liver tissue. Results Compared with the model group,in RJTS treatment groups,liver indexes were reduced apparently and the histopathological changes of liver were improved obvi-ously. RJTS reduced significantly serum ALT and AST levels(P ﹤ 0. 05,P ﹤ 0. 01),reduced the levels of MDA,GSH,IL - 6 and TNF - α in liver tissue(P ﹤ 0. 05,P ﹤ 0. 01)and up - regulated GSH and SOD activi-ty(P ﹤ 0. 05,P ﹤ 0. 01). Conclusion RJTS presents the protective effect on liver injury in mice and its pro-tective mechanism is relevant with reducing free radical production,improving antioxidant enzyme activity and reducing inflammatory cell factors,TNF - α and IL - 6.