检验医学与临床
檢驗醫學與臨床
검험의학여림상
Laboratory Medicine and Clinic
2015年
17期
2582-2583,2586
,共3页
荧光原位杂交技术%乳腺原发癌%淋巴结转移癌%HER-2基因
熒光原位雜交技術%乳腺原髮癌%淋巴結轉移癌%HER-2基因
형광원위잡교기술%유선원발암%림파결전이암%HER-2기인
fluorescence in situ hybridization%primary breast cancer%lymph node metastasis%HER-2 gene
目的：探讨使用荧光原位杂交技术检测乳腺原发癌和淋巴结转移癌 H ER‐2基因的表达及意义。方法选取海口市人民医院2013年1月至2014年1月收治的乳腺原发癌患者40例作为研究对象，对其临床资料进行回顾性分析。应用荧光原位杂交技术（FISH）与免疫组织化学技术（IHC）对所有患者手术切除标本的 HER‐2基因表达予以检测。结果 FISH阳性率为42．5％，IHC为57．5％；IHC阴性时与 FISH符合率为88．2％，IHC （＋）与FISH符合率为37．5％，IHC（＋＋）与FISH符合率为83．3％，IHC（＋＋ ＋）与FISH符合率为100．0％。淋巴结转移阳性率为66．67％，未转移阳性率为32．14％，对比差异有统计学意义（P＜0．05）。结论 IHC对 HER‐2基因予以检测时若结果为阴性或者强阳性则与FISH符合率较高，同时HER‐2基因表达与腋淋巴结转移之间呈正相关关系。
目的：探討使用熒光原位雜交技術檢測乳腺原髮癌和淋巴結轉移癌 H ER‐2基因的錶達及意義。方法選取海口市人民醫院2013年1月至2014年1月收治的乳腺原髮癌患者40例作為研究對象，對其臨床資料進行迴顧性分析。應用熒光原位雜交技術（FISH）與免疫組織化學技術（IHC）對所有患者手術切除標本的 HER‐2基因錶達予以檢測。結果 FISH暘性率為42．5％，IHC為57．5％；IHC陰性時與 FISH符閤率為88．2％，IHC （＋）與FISH符閤率為37．5％，IHC（＋＋）與FISH符閤率為83．3％，IHC（＋＋ ＋）與FISH符閤率為100．0％。淋巴結轉移暘性率為66．67％，未轉移暘性率為32．14％，對比差異有統計學意義（P＜0．05）。結論 IHC對 HER‐2基因予以檢測時若結果為陰性或者彊暘性則與FISH符閤率較高，同時HER‐2基因錶達與腋淋巴結轉移之間呈正相關關繫。
목적：탐토사용형광원위잡교기술검측유선원발암화림파결전이암 H ER‐2기인적표체급의의。방법선취해구시인민의원2013년1월지2014년1월수치적유선원발암환자40례작위연구대상，대기림상자료진행회고성분석。응용형광원위잡교기술（FISH）여면역조직화학기술（IHC）대소유환자수술절제표본적 HER‐2기인표체여이검측。결과 FISH양성솔위42．5％，IHC위57．5％；IHC음성시여 FISH부합솔위88．2％，IHC （＋）여FISH부합솔위37．5％，IHC（＋＋）여FISH부합솔위83．3％，IHC（＋＋ ＋）여FISH부합솔위100．0％。림파결전이양성솔위66．67％，미전이양성솔위32．14％，대비차이유통계학의의（P＜0．05）。결론 IHC대 HER‐2기인여이검측시약결과위음성혹자강양성칙여FISH부합솔교고，동시HER‐2기인표체여액림파결전이지간정정상관관계。
Objective To investigate the expression and significance of HER‐2 gene expression in primary breast cancer and lymph node metastatic carcinoma by using fluorescence in situ hybridization technique .Methods 40 cases of primary breast cancer in the Haikou Municipal People′s Hospital from January 2013 to January 2014 were selected as the research subjects and their clinical data were retrospectively analyzed .The fluorescence in situ hybrid‐ization (FISH) technique and immunohistochemical (IHC) techniques were used to detect the expression of HER‐2 gene in operation excision specimens of all patients .Results The positive rate of FISH was 42 .5% ,which of IHC was 57 .5% ;in negative IHC ,the coincidence rate with FISH was 88 .2% ,the coincidence rate of IHC (+ ) with FISH was 37 .5% ,which of IHC (+ + ) with FISH was 83 .3% and which of IHC (+ + + ) with FISH was 100% . The lymph node metastasis positive rate was 66 .67% ,non‐lymph node metastasis positive rate was 32 .14% ,the difference was statistically significant(P<0 .05) .Conclusion Detecting HER‐2 gene by IHC ,if the result is negative or strongly positive ,the coincidence rate with FISH is higher ,at the same time the HER‐2 gene expression is posi‐tivele correlated with axillary lymph node metastasis .
