CAJ | 학술논문

目的了解肝细胞生长因子(HGF)及其受体(C-MET)在翼状胬肉组织中的异常表达,探讨其对翼状胬肉的作用和影响.方法临床病例对照研究.对2013年10月至2014年6月在北仑区人民医院眼科,应用免疫荧光组织化学技术对33例翼状胬肉患者切除的翼状胬肉组织进行HGF及C-MET受体的检测,并与15例正常球结膜组织进行对照、分析.结果免疫荧光组织化学染色显示,在正常球结膜组织、翼状胬肉组织中,HGF及其C-MET受体在鳞状上皮层及上皮下组织中均可见阳性表达,但表达均以鳞状上皮细胞层为主,尤其以上皮层中临近上皮下间质的基底细胞表达最为强烈.15例正常球结膜组织HGF的平均光密度值为0.2644±0.02629,33例翼状胬肉组HGF的平均光密度值为0.3167±0.07221,差异有统计学意义(t=3.658,P＜0.05);15例正常球结膜组织C-MET受体的平均光密度值为0.115±0.02086,30例翼状胬肉组C-MET受体的平均光密度值为0.19017±0.039118,差异有统计学意义(t =6.938,P＜0.05).结论翼状胬肉组织中HGF及其C-MET受体表达的异常增加,提示肝细胞生长因子与翼状胬肉的生成发展相关.无论正常球结膜组织或翼状胬肉组织,HGF及其C-MET受体均在鳞状上皮细胞层的基底细胞表达最强烈,故推测:细胞因子在球结膜鳞状上皮层的异常表达及其对上皮下组织的调控是翼状胬肉发生发展中的主要影响因素之一.
목적 료해간세포생장인자(HGF)급기수체(C-MET)재익상노육조직중적이상표체,탐토기대익상노육적작용화영향.방법 림상병례대조연구.대2013년10월지2014년6월재북륜구인민의원안과,응용면역형광조직화학기술대33례익상노육환자절제적익상노육조직진행HGF급C-MET수체적검측,병여15례정상구결막조직진행대조、분석.결과 면역형광조직화학염색현시,재정상구결막조직、익상노육조직중,HGF급기C-MET수체재린상상피층급상피하조직중균가견양성표체,단표체균이린상상피세포층위주,우기이상피층중림근상피하간질적기저세포표체최위강렬.15례정상구결막조직HGF적평균광밀도치위0.2644±0.02629,33례익상노육조HGF적평균광밀도치위0.3167±0.07221,차이유통계학의의(t=3.658,P＜0.05);15례정상구결막조직C-MET수체적평균광밀도치위0.115±0.02086,30례익상노육조C-MET수체적평균광밀도치위0.19017±0.039118,차이유통계학의의(t =6.938,P＜0.05).결론 익상노육조직중HGF급기C-MET수체표체적이상증가,제시간세포생장인자여익상노육적생성발전상관.무론정상구결막조직혹익상노육조직,HGF급기C-MET수체균재린상상피세포층적기저세포표체최강렬,고추측:세포인자재구결막린상상피층적이상표체급기대상피하조직적조공시익상노육발생발전중적주요영향인소지일.
Objective To investigate the expression of hepatocyte growth factor (HGF) and its receptor (C-MET) in the tissue of pterygium to discusses their function in the pathogensis of pterygium.Methods Thirty-three pterygium specimens were obtained from 33 pterygium patients during the operations and 15 normal conjunctival specimens were taken from the healthy eyes by the eye bank of the first affiliated hospital,Zhejiang university school of medicine during the keratoplasty.Pathological examinations were performed under the optical microscope.The expression of HGF and C-MET receptor of pterygium and normal conjunctiva were examined using immunofluorescence histochemicaltechnique.The independent sample test was used for statistic analysis.Results The present study showed that the HGF and C-MET receptor were expressed in the epithelial and subepithelial conjunctival tissues of both groups.And the expression of all was mainly in the squamous epithelial cells layer,particularly the basal cells which are adjacent to subepithelial mesenchyme.The average optical density (mean density) of HGF of normal conjunctival tissue was 0.2644 ± 0.02629,while the mean density of pterygium group was 0.3167 ± 0.07221.The expression of HGF between pterygium and normal conjunctival tissues was significantly differently (t =3.658,P ＜0.05);the mean density of C-MET receptor in normal and pterygium conjunctival tissues was 0.115 ± 0.02086 and 0.19017 ± 0.039118 respectively.The expression of C-MET was significantly difference between the two groups (t =6.938,P ＜0.05).Conclusions The increased expression of HGF and its C-MET receptor in pterygium indicates that HGF may play an integrated role in the development of pterygium.Further more,there gulating effect of cytokines to subepithelial tissues,which is abnormally expressed from the squamous epithelial cell layer,may be one of the main influencing factors of pterygium development.