CAJ | 학술논문

目的：建立 HPLC 法同时测定痛经贴剂中柚皮苷、橙皮苷和新橙皮苷的含量。方法采用高效液相色谱法，以 Cosmosil-C18为色谱柱（5μm，4．6 mm ×250 mm）；乙腈—0．1％磷酸（21∶79）为流动相；流速为1．0 mL· min －1；检测波长为283 nm；柱温为25℃；进样量为10μL。结果柚皮苷浓度在10．63～340 mg·L －1，橙皮苷浓度在7．81～250 mg·L －1，新橙皮苷浓度在8．13～260 mg·L －1范围内线性关系良好，平均加样回收率分别为98．01％、97．90％和98．60％。结论该方法简单、准确、可靠，可用于痛经贴剂的质量控制。
목적：건립 HPLC 법동시측정통경첩제중유피감、등피감화신등피감적함량。방법채용고효액상색보법，이 Cosmosil-C18위색보주（5μm，4．6 mm ×250 mm）；을정—0．1％린산（21∶79）위류동상；류속위1．0 mL· min －1；검측파장위283 nm；주온위25℃；진양량위10μL。결과유피감농도재10．63～340 mg·L －1，등피감농도재7．81～250 mg·L －1，신등피감농도재8．13～260 mg·L －1범위내선성관계량호，평균가양회수솔분별위98．01％、97．90％화98．60％。결론해방법간단、준학、가고，가용우통경첩제적질량공제。
Objective To establish a method for simultaneous determination of naringin,hesperidin and neohesperidin in dysmenorrhea patches by HPLC.Methods The HPLC method was validated using a Cosmosil C18 analytical column(5 μm,4.6 mm ×250 mm)and the mobile phase was acetonitrile -0.1% phosphoric acid (21∶79)at a flow rate of 1.0 mL·min -1 with UV detection at 283 nm.The column temperature was kept at 25℃ and the injection volume was 10 μL.Results The linear ranges of naringin,hesperidin and neo-hesperidin were 10.63 ~340 mg·L -1 ,7.81 ~250 mg·L -1 and 8.13 ~260 mg·L -1 ,respectively.The average sample recovery rates were 98.01 %,97.90 % and 98.60 %,respectively.Conclusion The method is simple,accurate and reliable,which can be used for quality control of dysmenorrhea patches.