安徽医药
安徽醫藥
안휘의약
Anhui Medical and Pharmaceutical Journal
2015年
10期
1869-1872
,共4页
复方九节茶乳膏%高效液相色谱法%薄层色谱法%反丁烯二酸%迷迭香酸%异嗪皮啶%冰片
複方九節茶乳膏%高效液相色譜法%薄層色譜法%反丁烯二痠%迷迭香痠%異嗪皮啶%冰片
복방구절다유고%고효액상색보법%박층색보법%반정희이산%미질향산%이진피정%빙편
compound Sarcandra Glabra ointment%HPLC%TLC%fumaric acid%rosmarinci acid%isofraxidin%synthetic borneol
目的:建立复方九节茶乳膏质量标准。方法采用薄层色谱法对九节茶和冰片进行鉴别;HPLC 双波长法测定复方九节茶乳膏中反丁烯二酸与迷迭香酸含量。采用 Kromasil C18色谱柱(250 mm ×4.6 mm,5μm),流动相为0.1%甲酸乙腈溶液(B)—0.1%甲酸水溶液(D),梯度洗脱[0~10 min,5% B→20% B;10~25 min,20% B;25~30 min,20% B→5% B;30~35 min,5% B],流速为1.0 mL·min -1,检测波长为210 nm 和330 nm,柱温为30℃。结果薄层鉴别斑点清晰,阴性无干扰;反丁烯二酸和迷迭香酸线性范围分别为6.53~65.28 mg·L -1(r =0.9999)和4.26~42.64 mg·L -1(r =0.9995);平均加样回收率分别为98.3%(RSD =0.82%)和100.9%(RSD =0.63%)。结论该方法简单、准确、重复性好,能有效的控制该制剂的质量。
目的:建立複方九節茶乳膏質量標準。方法採用薄層色譜法對九節茶和冰片進行鑒彆;HPLC 雙波長法測定複方九節茶乳膏中反丁烯二痠與迷迭香痠含量。採用 Kromasil C18色譜柱(250 mm ×4.6 mm,5μm),流動相為0.1%甲痠乙腈溶液(B)—0.1%甲痠水溶液(D),梯度洗脫[0~10 min,5% B→20% B;10~25 min,20% B;25~30 min,20% B→5% B;30~35 min,5% B],流速為1.0 mL·min -1,檢測波長為210 nm 和330 nm,柱溫為30℃。結果薄層鑒彆斑點清晰,陰性無榦擾;反丁烯二痠和迷迭香痠線性範圍分彆為6.53~65.28 mg·L -1(r =0.9999)和4.26~42.64 mg·L -1(r =0.9995);平均加樣迴收率分彆為98.3%(RSD =0.82%)和100.9%(RSD =0.63%)。結論該方法簡單、準確、重複性好,能有效的控製該製劑的質量。
목적:건립복방구절다유고질량표준。방법채용박층색보법대구절다화빙편진행감별;HPLC 쌍파장법측정복방구절다유고중반정희이산여미질향산함량。채용 Kromasil C18색보주(250 mm ×4.6 mm,5μm),류동상위0.1%갑산을정용액(B)—0.1%갑산수용액(D),제도세탈[0~10 min,5% B→20% B;10~25 min,20% B;25~30 min,20% B→5% B;30~35 min,5% B],류속위1.0 mL·min -1,검측파장위210 nm 화330 nm,주온위30℃。결과박층감별반점청석,음성무간우;반정희이산화미질향산선성범위분별위6.53~65.28 mg·L -1(r =0.9999)화4.26~42.64 mg·L -1(r =0.9995);평균가양회수솔분별위98.3%(RSD =0.82%)화100.9%(RSD =0.63%)。결론해방법간단、준학、중복성호,능유효적공제해제제적질량。
Objective To establish the quality standard for compound Sarcandra Glabra ointment.Methods Sarcandra Glabra and synthetic borneol in the formula were identified qualitatively by TLC and the contents of fumaric acid and rosmarinci acid were deter-mined by HPLC.An Kromasil C18 column(250 mm ×4.6 mm,5μm)was adopted at 30℃.The mobile phases were 0.1% formic acid acetonitrile solution (B)and 0.1% formic acid water solution(D)with a gradient elution mode(0 ~10 min,5%B→20% B;10 ~25 min,20% B;25 ~30 min,20% B→5% B;30 ~35 min,5% B)at the flow rate of 1.0 mL·min -1 .And the detection wavelength was 210 nm and 330 nm.Results Sarcandra Glabra and synthetic borneol could be detected by TLC.Fumaric acid and rosmarinci acid were well separated within a linear range of 6.53 ~65.28(r =0.999 9)and 4.26 ~42.64 mg·L -1 (r =0.999 5),respectively.The average recoveries were 98.3% with RSD 0.82%,100.9% with RSD 0.63%,respectively.Conclusions The method was accurate, sensitive and reproducible,which can be used for its quality control.