安徽医药
安徽醫藥
안휘의약
Anhui Medical and Pharmaceutical Journal
2015年
10期
1857-1860
,共4页
姚红锐%龙子江%赵刚%杨燕%江震
姚紅銳%龍子江%趙剛%楊燕%江震
요홍예%룡자강%조강%양연%강진
毛菊苣%有效部位%含药血清%降脂作用%初步机制
毛菊苣%有效部位%含藥血清%降脂作用%初步機製
모국거%유효부위%함약혈청%강지작용%초보궤제
Cichorium glundulosum%effective parts%medicated serum%lipid-lowering effect%preliminary mechanism
目的:在整体水平上,研究毛菊苣有效部位含药血清降脂作用及初步机制。方法利用 MTT 法确定血清的加入量以及尼罗红染色建立 HepG2细胞高脂模型,在此基础上,检测细胞内的关键性指标甘油三酯(TG)和脂滴的含量,最后通过ELISA 酶联免疫吸附法分别从游离脂肪酸的吸收、氧化、TG 的合成方面对毛菊苣降脂有效部位含药血清的降脂机制进行初步的探讨。结果通过 MTT 实验和尼罗红染色及流式定量分析表明,脂肪乳浓度为总体积的6%时,造模组细胞内的脂滴含量较正常对照组明显增加,具有显著性差异(P <0.01、0.001);不同模型的大鼠含药血清均可显著性地降低 HepG2细胞内 TG(P<0.05)和脂滴(P <0.01、0.001)的含量,并且正常状态下的含药血清比病理状态下的含药血清降脂效果显著;同时对 TG 合成、氧化、吸收通路中的关键酶 GPAT1-2(P <0.05)、PPARγ(P <0.05、0.01)、FATP5(P <0.01)三个指标上的检测表明正常状态下的含药血清与高脂模型组相比具有显著性差异。结论大鼠病理状态下(高脂模型)与正常生理状态下的毛菊苣降脂有效部位含药血清具有显著性地降低人肝癌 HepG2细胞高脂模型的作用,并且后者较前者降脂效果明显,其机制可能与减少GPAT1-2的含量、促进 PPARγ的表达和抑制 FATP5的生成有关。
目的:在整體水平上,研究毛菊苣有效部位含藥血清降脂作用及初步機製。方法利用 MTT 法確定血清的加入量以及尼囉紅染色建立 HepG2細胞高脂模型,在此基礎上,檢測細胞內的關鍵性指標甘油三酯(TG)和脂滴的含量,最後通過ELISA 酶聯免疫吸附法分彆從遊離脂肪痠的吸收、氧化、TG 的閤成方麵對毛菊苣降脂有效部位含藥血清的降脂機製進行初步的探討。結果通過 MTT 實驗和尼囉紅染色及流式定量分析錶明,脂肪乳濃度為總體積的6%時,造模組細胞內的脂滴含量較正常對照組明顯增加,具有顯著性差異(P <0.01、0.001);不同模型的大鼠含藥血清均可顯著性地降低 HepG2細胞內 TG(P<0.05)和脂滴(P <0.01、0.001)的含量,併且正常狀態下的含藥血清比病理狀態下的含藥血清降脂效果顯著;同時對 TG 閤成、氧化、吸收通路中的關鍵酶 GPAT1-2(P <0.05)、PPARγ(P <0.05、0.01)、FATP5(P <0.01)三箇指標上的檢測錶明正常狀態下的含藥血清與高脂模型組相比具有顯著性差異。結論大鼠病理狀態下(高脂模型)與正常生理狀態下的毛菊苣降脂有效部位含藥血清具有顯著性地降低人肝癌 HepG2細胞高脂模型的作用,併且後者較前者降脂效果明顯,其機製可能與減少GPAT1-2的含量、促進 PPARγ的錶達和抑製 FATP5的生成有關。
목적:재정체수평상,연구모국거유효부위함약혈청강지작용급초보궤제。방법이용 MTT 법학정혈청적가입량이급니라홍염색건립 HepG2세포고지모형,재차기출상,검측세포내적관건성지표감유삼지(TG)화지적적함량,최후통과ELISA 매련면역흡부법분별종유리지방산적흡수、양화、TG 적합성방면대모국거강지유효부위함약혈청적강지궤제진행초보적탐토。결과통과 MTT 실험화니라홍염색급류식정량분석표명,지방유농도위총체적적6%시,조모조세포내적지적함량교정상대조조명현증가,구유현저성차이(P <0.01、0.001);불동모형적대서함약혈청균가현저성지강저 HepG2세포내 TG(P<0.05)화지적(P <0.01、0.001)적함량,병차정상상태하적함약혈청비병리상태하적함약혈청강지효과현저;동시대 TG 합성、양화、흡수통로중적관건매 GPAT1-2(P <0.05)、PPARγ(P <0.05、0.01)、FATP5(P <0.01)삼개지표상적검측표명정상상태하적함약혈청여고지모형조상비구유현저성차이。결론대서병리상태하(고지모형)여정상생리상태하적모국거강지유효부위함약혈청구유현저성지강저인간암 HepG2세포고지모형적작용,병차후자교전자강지효과명현,기궤제가능여감소GPAT1-2적함량、촉진 PPARγ적표체화억제 FATP5적생성유관。
Objective To explore lipid-lowering effect of the effective parts of Cichorium glundulosum and its preliminary mechanism. Methods MTT method was used to determine the amount of serum,as well as Nile red staining to establish hyperlipidemia model of HepG2 cells.On this basis,the content of key index TG and the lipid droplets were determined.Finally,ELISA enzyme-linked immu-nosorbent assay was used to make a preliminary study of the lipid-lowering mechanism of Cichorium glundulosum effective parts from the aspects of the absorption,oxidation and TG synthesis of free fatty acid.Results It was showed that,through MTT experiment and Nile red staining and the flow type quantitative analysis,fat drops content of module cell was obviously increased more than that of con-trol group and had significant difference (P <0.01 and 0.001)when fat breast concentration was 6% of the total volume;content of TG (P <0.05)and fat drops (P <0.01 and 0.001)of HepG2 cells were significantly reduced in different models of rat,and medicated serum in normal state had more significant lipid-lowering effect than that in pathological state.And tests of the three indexes GPAT1 -2 (P <0.05),PPARγ(P <0.05 and 0.01),FATP5(P <0.01)showed that there was significant difference between medicated serum in normal state group and that in high lipid model group.Conclusions Medicated serum with the effective parts of Cichorium glundulo-sum in pathological state (high lipid model)or in normal state can both significantly lower hyperlipidemia model of human hepatoma cell line HepG2 cells and the latter had more significant lipid-lowering effect,the mechanism of which may be related to reducing the content of GPAT1 -2 ,promoting the expression of PPARγand inhibiting the FATP5 generation.