CAJ | 학술논문

采用双夹心磁性微球免疫荧光显微法对样品中的癌胚抗原进行分离与检测。首先用EDC/NHS对磁性微球表面的羧基进行活化后偶联抗癌胚抗原单克隆抗体mAbCEA-C3制成免疫磁珠，再对样品中的CEA进行捕获，并进行免疫标记，最后用荧光显微镜进行检测。考察洗涤次数和免疫时间等因素对实验的影响，在最优条件下对CEA进行检测，结果表明：随着CEA浓度的增加荧光强度逐渐增强，检测限为9.67 ng/mL，可实现对CEA的定性和半定量测定。该方法操作简单快捷，样品用量少，选择性好，可应用于临床检验。
채용쌍협심자성미구면역형광현미법대양품중적암배항원진행분리여검측。수선용EDC/NHS대자성미구표면적최기진행활화후우련항암배항원단극륭항체mAbCEA-C3제성면역자주，재대양품중적CEA진행포획，병진행면역표기，최후용형광현미경진행검측。고찰세조차수화면역시간등인소대실험적영향，재최우조건하대CEA진행검측，결과표명：수착CEA농도적증가형광강도축점증강，검측한위9.67 ng/mL，가실현대CEA적정성화반정량측정。해방법조작간단쾌첩，양품용량소，선택성호，가응용우림상검험。
A novel immunoassay for the determination of CEA was established by combining a fluoroimmunoassay and immuno?magnetic separation. Carboxyl groups on the magnetic beads were first activated with a mixture of EDC and NHS to give reactive succin?imide esters. The mAbCEA-C3 was then passed over the surface and esters react spontaneously with amino groups to link mAbCEA-C3 covalently to magnetic beads to form Immonumagnetic beads(IBMs). In the mixture samples, CEA was captured by mAbCEA-C 3 with high specificity and affinity. The conditions, such as washing times and immune reaction time were optimized. The final choice of immune time is 45min, three times of washing. The results indicated that the fluorescence intensity increased with the increment of CEA concentration in the optimal condition. The LOD is 9.67 ng/mL. Qualitative and semiquantitative detection of CEA was conducted based on the combination of IBMs with fluoroimmunoassay. The method is simple and sensitive and could be a possible application for the detection of CEA in clinical diagnostics.