北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
Journal of Beijing University of Traditional Chinese Medicine
2015年
7期
481-485
,共5页
宋艳%赵国桢%汪德生%赵百孝%嵇波%张鹤%毛颖秋%张平%许咏思%刘亚莉%路雅雯%戴健%李莹辉
宋豔%趙國楨%汪德生%趙百孝%嵇波%張鶴%毛穎鞦%張平%許詠思%劉亞莉%路雅雯%戴健%李瑩輝
송염%조국정%왕덕생%조백효%혜파%장학%모영추%장평%허영사%류아리%로아문%대건%리형휘
针刺%模拟失重%肝脏损伤%血清谷丙转氨酶%一氧化氮%大鼠
針刺%模擬失重%肝髒損傷%血清穀丙轉氨酶%一氧化氮%大鼠
침자%모의실중%간장손상%혈청곡병전안매%일양화담%대서
acupuncture%simulated weightlessness%liver injury%ALT%NO%rats
目的：观察预针刺和针刺治疗对模拟失重大鼠肝脏组织结构、功能和一氧化氮（NO）水平的影响，探讨不同时机介入电针治疗对模拟失重大鼠肝脏损伤的防护机制。方法将20只雄性清洁级 Wistar 大鼠随机分为正常组、模型组、预针刺组和针刺组，每组5只。正常组不做任何处理，自由活动饲养4周；模型组、预针刺组和针刺组大鼠尾部悬吊4周制造模拟失重大鼠模型，预针刺组在尾吊前1周，电针刺激双侧“肾俞”“脾俞”和“三阴交”穴，每次30 min，每日1次，共治疗7次；针刺组针刺治疗与尾部悬吊同时开始，大鼠尾部悬吊过程中，电针刺激“肾俞”“脾俞”和“三阴交”穴，每次30 min，隔日治疗1次，共针刺14次。采用 HE 染色法物镜下观察各组大鼠肝脏组织结构的变化，比色法检测各组大鼠血清谷丙转氨酶（ALT）活性及肝脏组织一氧化氮（NO）含量。结果肝脏组织 HE 染色切片观察显示：模型组大鼠肝脏中央静脉周围出现大面积的肝细胞变性；经电针治疗后，大鼠肝脏组织结构病理变化较模型组有所改善，且预针刺组疗效优于针刺组。与正常组比较，模型组大鼠血清 ALT 活性和肝脏组织 NO 含量显著升高（P ＜0．05，P ＜0．01）；与模型组比较，预针刺组大鼠血清 ALT 活性及肝脏组织 NO 含量显著降低（均 P ＜0．05），针刺组以上指标变化不显著。与预针刺组比较，针刺组 ALT 活性有升高趋势，NO 含量显著升高（P ＜0．01）。结论预针刺较针刺治疗更能有效地缓解大鼠尾部悬吊引起的肝脏组织结构和功能的损伤，其机理可能与NO 有关。
目的：觀察預針刺和針刺治療對模擬失重大鼠肝髒組織結構、功能和一氧化氮（NO）水平的影響，探討不同時機介入電針治療對模擬失重大鼠肝髒損傷的防護機製。方法將20隻雄性清潔級 Wistar 大鼠隨機分為正常組、模型組、預針刺組和針刺組，每組5隻。正常組不做任何處理，自由活動飼養4週；模型組、預針刺組和針刺組大鼠尾部懸弔4週製造模擬失重大鼠模型，預針刺組在尾弔前1週，電針刺激雙側“腎俞”“脾俞”和“三陰交”穴，每次30 min，每日1次，共治療7次；針刺組針刺治療與尾部懸弔同時開始，大鼠尾部懸弔過程中，電針刺激“腎俞”“脾俞”和“三陰交”穴，每次30 min，隔日治療1次，共針刺14次。採用 HE 染色法物鏡下觀察各組大鼠肝髒組織結構的變化，比色法檢測各組大鼠血清穀丙轉氨酶（ALT）活性及肝髒組織一氧化氮（NO）含量。結果肝髒組織 HE 染色切片觀察顯示：模型組大鼠肝髒中央靜脈週圍齣現大麵積的肝細胞變性；經電針治療後，大鼠肝髒組織結構病理變化較模型組有所改善，且預針刺組療效優于針刺組。與正常組比較，模型組大鼠血清 ALT 活性和肝髒組織 NO 含量顯著升高（P ＜0．05，P ＜0．01）；與模型組比較，預針刺組大鼠血清 ALT 活性及肝髒組織 NO 含量顯著降低（均 P ＜0．05），針刺組以上指標變化不顯著。與預針刺組比較，針刺組 ALT 活性有升高趨勢，NO 含量顯著升高（P ＜0．01）。結論預針刺較針刺治療更能有效地緩解大鼠尾部懸弔引起的肝髒組織結構和功能的損傷，其機理可能與NO 有關。
목적：관찰예침자화침자치료대모의실중대서간장조직결구、공능화일양화담（NO）수평적영향，탐토불동시궤개입전침치료대모의실중대서간장손상적방호궤제。방법장20지웅성청길급 Wistar 대서수궤분위정상조、모형조、예침자조화침자조，매조5지。정상조불주임하처리，자유활동사양4주；모형조、예침자조화침자조대서미부현조4주제조모의실중대서모형，예침자조재미조전1주，전침자격쌍측“신유”“비유”화“삼음교”혈，매차30 min，매일1차，공치료7차；침자조침자치료여미부현조동시개시，대서미부현조과정중，전침자격“신유”“비유”화“삼음교”혈，매차30 min，격일치료1차，공침자14차。채용 HE 염색법물경하관찰각조대서간장조직결구적변화，비색법검측각조대서혈청곡병전안매（ALT）활성급간장조직일양화담（NO）함량。결과간장조직 HE 염색절편관찰현시：모형조대서간장중앙정맥주위출현대면적적간세포변성；경전침치료후，대서간장조직결구병리변화교모형조유소개선，차예침자조료효우우침자조。여정상조비교，모형조대서혈청 ALT 활성화간장조직 NO 함량현저승고（P ＜0．05，P ＜0．01）；여모형조비교，예침자조대서혈청 ALT 활성급간장조직 NO 함량현저강저（균 P ＜0．05），침자조이상지표변화불현저。여예침자조비교，침자조 ALT 활성유승고추세，NO 함량현저승고（P ＜0．01）。결론예침자교침자치료경능유효지완해대서미부현조인기적간장조직결구화공능적손상，기궤리가능여NO 유관。
Objective To observe the effect of needling on the structure,function and level of nitric oxide (NO)of the liver in rats with simulated weightlessness,so as to explore the protective mechanism of acu-puncture on liver injury.Methods Twenty male clean grade Wistar rats were randomly divided into nor-mal group,model group,pretreatment group and treatment group(each n =5).The rats of normal group received no treatment,and those of other three groups were tail -suspended for 4 weeks to establish weightlessness model.Electroacupuncture at the points of Shenshu (BL23),Pishu (BL20)and Sanyin-jiao (SP60)once daily for 30 min was applied one week ahead of tail-suspension in pre-treatment group, while in treatment group the needling treatment applied at the same three points once every other day for 14 d was carried out at the day when the rats was tail -suspended.The changes of hepatic histological structure were observed,and the activity of alanine aminotransferase(ALT)in serum and the content of NO in liver were measured.Results The hepatic cell degeneration were observed around the central vein in model group;the degree of degeneration relieved in both electroacupuncture treatment groups.Further-more,the curative effect of the pre-treatment group was better than the treatment group.Compared with normal group,the activity of ALT in serum and the content of NO in liver in model group increased signif-icantly (P <0.05,P <0.01).Compared with model group,the activity of ALT in serum and the con-tent of NO in liver in the pre-treatment group significantly reduced(P <0.05),however,those in treat-ment group had no obvious difference.Compared with pre-treatment group,the activity of ALT tended to increase and the content of NO increased significantly(P <0.01)in the acupuncture group.Conclusion The effects of relieving hepatic injury and dysfunction induced by weightlessness,which potentially was related to the content of NO,in the pre-treatment group were superior to those in the treatment group.
