北京中医药大学学报
北京中醫藥大學學報
북경중의약대학학보
Journal of Beijing University of Traditional Chinese Medicine
2015年
8期
515-518,528
,共5页
赵荣华%谢鸣%刘进娜%李聪%张敬升%王帮众%么元超
趙榮華%謝鳴%劉進娜%李聰%張敬升%王幫衆%麽元超
조영화%사명%류진나%리총%장경승%왕방음%요원초
肝郁%脾虚%肝郁脾虚%柴疏四君子汤%旷场实验%大鼠
肝鬱%脾虛%肝鬱脾虛%柴疏四君子湯%曠場實驗%大鼠
간욱%비허%간욱비허%시소사군자탕%광장실험%대서
liver-stagnation%spleen-deficiency%liver-stagnation and spleen-deficiency%Chaihu Sijunzi Decoction%open-fieldtest%rats
目的：比较肝郁证、脾虚证和肝郁脾虚证模型大鼠的行为学变化及柴疏四君子汤对其作用的异同。方法大鼠随机分为正常组、肝郁组、脾虚组、肝郁脾虚组、肝郁干预组、脾虚干预组、肝郁脾虚干预组共7组，每组13只。3个证候模型组及各干预组大鼠分别采用慢性束缚、过度疲劳＋饮食失节、慢性束缚＋过度疲劳＋饮食失节法造模，正常组不予处理；于造模第2周末，3个证候干预组大鼠按2．86 g／kg 分别给予柴疏四君子汤灌胃，连续2周；各模型组和正常组给予等量蒸馏水。实验第14天和第28天，旷场实验法观察各组大鼠的行为特征，记录爬格数、直立次数、中央格停留时间、修饰时间及测算活动里程数。结果实验2周末时，与正常组相比，肝郁组和脾虚组大鼠爬格数、直立次数和里程数均显著升高（P ＜0．05），肝郁脾虚组则均显著降低（P ＜0．05）；肝郁组和肝郁脾虚组中央格停留时间显著升高、修饰时间降低（P ＜0．05），脾虚组变化与之相反（P ＜0.05）。实验4周末时，与正常组相比，3个证候模型组大鼠爬格数和里程数均显著降低（P ＜0．05），其中肝郁组和肝郁脾虚组还见直立次数和修饰时间显著降低（P ＜0．05），中央格停留时间显著升高（P ＜0．05），脾虚组的相关指标变化相反。中药干预2周后的变化：与相应模型组相比，肝郁干预组大鼠爬格数、里程数显著升高（P ＜0．05），中央格停留时间显著降低（P ＜0．05）；脾虚干预组还见修饰时间显著升高（P ＜0．05），而直立次数显著降低（P ＜0．05）；肝郁脾虚干预组各参数均显著升高（P ＜0．05）。结论3个证候模型大鼠在自主活动方面均存在不同程度的异常，其中肝郁证大鼠中枢的兴奋性由强转弱，脾虚证大鼠呈中枢神经系统反应失调，肝郁脾虚证大鼠呈现抑制渐进加重的趋势。柴疏四君子汤对3个证候模型的行为学指标均有一定的改善作用，但以对肝郁脾虚证的综合干预效应最优。
目的：比較肝鬱證、脾虛證和肝鬱脾虛證模型大鼠的行為學變化及柴疏四君子湯對其作用的異同。方法大鼠隨機分為正常組、肝鬱組、脾虛組、肝鬱脾虛組、肝鬱榦預組、脾虛榦預組、肝鬱脾虛榦預組共7組，每組13隻。3箇證候模型組及各榦預組大鼠分彆採用慢性束縳、過度疲勞＋飲食失節、慢性束縳＋過度疲勞＋飲食失節法造模，正常組不予處理；于造模第2週末，3箇證候榦預組大鼠按2．86 g／kg 分彆給予柴疏四君子湯灌胃，連續2週；各模型組和正常組給予等量蒸餾水。實驗第14天和第28天，曠場實驗法觀察各組大鼠的行為特徵，記錄爬格數、直立次數、中央格停留時間、脩飾時間及測算活動裏程數。結果實驗2週末時，與正常組相比，肝鬱組和脾虛組大鼠爬格數、直立次數和裏程數均顯著升高（P ＜0．05），肝鬱脾虛組則均顯著降低（P ＜0．05）；肝鬱組和肝鬱脾虛組中央格停留時間顯著升高、脩飾時間降低（P ＜0．05），脾虛組變化與之相反（P ＜0.05）。實驗4週末時，與正常組相比，3箇證候模型組大鼠爬格數和裏程數均顯著降低（P ＜0．05），其中肝鬱組和肝鬱脾虛組還見直立次數和脩飾時間顯著降低（P ＜0．05），中央格停留時間顯著升高（P ＜0．05），脾虛組的相關指標變化相反。中藥榦預2週後的變化：與相應模型組相比，肝鬱榦預組大鼠爬格數、裏程數顯著升高（P ＜0．05），中央格停留時間顯著降低（P ＜0．05）；脾虛榦預組還見脩飾時間顯著升高（P ＜0．05），而直立次數顯著降低（P ＜0．05）；肝鬱脾虛榦預組各參數均顯著升高（P ＜0．05）。結論3箇證候模型大鼠在自主活動方麵均存在不同程度的異常，其中肝鬱證大鼠中樞的興奮性由彊轉弱，脾虛證大鼠呈中樞神經繫統反應失調，肝鬱脾虛證大鼠呈現抑製漸進加重的趨勢。柴疏四君子湯對3箇證候模型的行為學指標均有一定的改善作用，但以對肝鬱脾虛證的綜閤榦預效應最優。
목적：비교간욱증、비허증화간욱비허증모형대서적행위학변화급시소사군자탕대기작용적이동。방법대서수궤분위정상조、간욱조、비허조、간욱비허조、간욱간예조、비허간예조、간욱비허간예조공7조，매조13지。3개증후모형조급각간예조대서분별채용만성속박、과도피로＋음식실절、만성속박＋과도피로＋음식실절법조모，정상조불여처리；우조모제2주말，3개증후간예조대서안2．86 g／kg 분별급여시소사군자탕관위，련속2주；각모형조화정상조급여등량증류수。실험제14천화제28천，광장실험법관찰각조대서적행위특정，기록파격수、직립차수、중앙격정류시간、수식시간급측산활동리정수。결과실험2주말시，여정상조상비，간욱조화비허조대서파격수、직립차수화리정수균현저승고（P ＜0．05），간욱비허조칙균현저강저（P ＜0．05）；간욱조화간욱비허조중앙격정류시간현저승고、수식시간강저（P ＜0．05），비허조변화여지상반（P ＜0.05）。실험4주말시，여정상조상비，3개증후모형조대서파격수화리정수균현저강저（P ＜0．05），기중간욱조화간욱비허조환견직립차수화수식시간현저강저（P ＜0．05），중앙격정류시간현저승고（P ＜0．05），비허조적상관지표변화상반。중약간예2주후적변화：여상응모형조상비，간욱간예조대서파격수、리정수현저승고（P ＜0．05），중앙격정류시간현저강저（P ＜0．05）；비허간예조환견수식시간현저승고（P ＜0．05），이직립차수현저강저（P ＜0．05）；간욱비허간예조각삼수균현저승고（P ＜0．05）。결론3개증후모형대서재자주활동방면균존재불동정도적이상，기중간욱증대서중추적흥강성유강전약，비허증대서정중추신경계통반응실조，간욱비허증대서정현억제점진가중적추세。시소사군자탕대3개증후모형적행위학지표균유일정적개선작용，단이대간욱비허증적종합간예효응최우。
Objective To compare the behavioral changes of different rat models of liver-stagnation pattern,spleen-deficiency pattern,and liver-stagnation and spleen-deficiency pattern and to explore the effects of Chaihu Sijunzi Decoction (Bupleurum with Four Gentlemen Decoction)on those rat models. Methods Male Wistar rats were randomly assigned into the normal group (group 1 ),liver-stagnation group (group 2 ),spleen-deficiency group (group 3 ),liver-stagnation with spleen-deficiency group (group 4),liver-stagnation intervention group (group 5),spleen-deficiency intervention group (group 6)and liver-stagnation with spleen-deficiency intervention group (group 7)with thirteen rats in each group.The models were established by using chronic restraint method in group 2 and 5,by using excessive fatigue plus irregular diet method in group 3 and 6,and by using chronic restraint-excessive fatigue-irregular diet method in group 4 and 7 for 4 weeks.At the end of the 15 th day,rats in the group 5,6 and 7 were fed with Chaihu Sijunzi Decoction per 2.86 g·kg -1 for two weeks,whereas the rats in the normal group and model groups 2,3 and 4 were fed with equivalent saline for two weeks.On the 14th and 28 th day,open-fieldtest was used to observe the crossed squares (index C),number of rearing (index N),time remaining in the central zone (index S),decorating time (index D)and the total distance of movement (index T)of all rats.Results On the 14th day,as compared with group 1,index C,index N and index T increased significantly in group 2 and 3 (P <0.05)and decreased significantly in group 4 (P <0.05).Index S increased significantly in group 2 and 4 (P <0.05)while it decreased significantly in group 3 (P <0.05).Index D decreased significantly in group 2 and 4(P <0.05)and increased significantly in group 3 (P <0.05).On the 28 th day,compared with group 1,index C,index T decreased significantly in group 2,3 and 4 (P <0.05 ),while index N and index D decreased significantly and index S increased significantly in group 2 and 4 (P <0.05),which were totally opposite to group 3.Compared with group 2,index C and index T increased significantly while index S decreased significantly in group 5 (P <0.05).Compared with group 3,index C,index D,and index T increased significantly while index N decreased significantly in group 6(P <0.05).Compared with group 4,the crossed squares,number of rearing,decorating time,and index T increased significantly in group 7 (P <0.05).Conclusion The autonomic activities were at different degrees in rat models of liver-stagnation pattern,spleen-deficiency pattern,and liver-stagnation with spleen-deficiency pattern.There were decreased CNS excitement in the liver-stagnation pattern,disordered central nervous response in the spleen-deficiency pattern,and inhibited central excitatory activities in the liver-stagnation and spleen-deficiency pattern.Chaihu Sijunzi Decoction can improve the autonomic activities of those rat models,es-pecially of the liver-stagnation with spleen-deficiency model.
