CAJ | 학술논문

目的：建立基于药物体系的栀子特征图谱质量表征关联分析方法与评价模式，有效精准地评价栀子饮片质量。方法运用所建立的 HPLC-PDA 法表征栀子药物体系特征图谱质量，基于特征图谱中特征峰的数量及所属化学类型对16批栀子饮片进行质的表征，基于特征图谱中特征指标性成分栀子苷、京尼平龙胆双糖苷和绿原酸的含量及环烯醚萜类（以栀子苷表征）、苯丙酸类（以绿原酸表征）的含量，同时采用香草醛－高氯酸显色法测定栀子总萜含量，三氯化铁－铁氰化钾显色法测定栀子总酚含量，对16批栀子饮片进行量的表征，并基于基准饮片将16批栀子饮片质与量的表征结果分别进行关联性分析。结果以批号6为基准，栀子饮片特征图谱中共含有特征峰9个，其中环烯醚萜成分特征峰4个，苯丙酸类成分特征峰5个，16批饮片色谱图中均含有此9个特征峰。批号3、4、7、8、13有效指标性成分总体含量高于基准批号6，批号7、1、9、4、3、12、5、14与基准批号6关联性最高，综合评价得出批号3、4、6、7优良度居前。结论建立了栀子饮片的 HPLC 特征图谱质量表征方法，并结合总酚及总萜含量测定对16批栀子饮片进行质量表征。所建立的基于药物体系的栀子特征图谱质量表征关联分析模式，关注了栀子饮片质量整体关联性与应用有效性，能有效精准地评价栀子饮片质量。
목적：건립기우약물체계적치자특정도보질량표정관련분석방법여평개모식，유효정준지평개치자음편질량。방법운용소건립적 HPLC-PDA 법표정치자약물체계특정도보질량，기우특정도보중특정봉적수량급소속화학류형대16비치자음편진행질적표정，기우특정도보중특정지표성성분치자감、경니평룡담쌍당감화록원산적함량급배희미첩류（이치자감표정）、분병산류（이록원산표정）적함량，동시채용향초철－고록산현색법측정치자총첩함량，삼록화철－철청화갑현색법측정치자총분함량，대16비치자음편진행량적표정，병기우기준음편장16비치자음편질여량적표정결과분별진행관련성분석。결과이비호6위기준，치자음편특정도보중공함유특정봉9개，기중배희미첩성분특정봉4개，분병산류성분특정봉5개，16비음편색보도중균함유차9개특정봉。비호3、4、7、8、13유효지표성성분총체함량고우기준비호6，비호7、1、9、4、3、12、5、14여기준비호6관련성최고，종합평개득출비호3、4、6、7우량도거전。결론건립료치자음편적 HPLC 특정도보질량표정방법，병결합총분급총첩함량측정대16비치자음편진행질량표정。소건립적기우약물체계적치자특정도보질량표정관련분석모식，관주료치자음편질량정체관련성여응용유효성，능유효정준지평개치자음편질량。
Objective To establish the quality representation and correlation analysis method based on the characteristic spectrum of medicinal system of raw decoction pieces of Zhizi (Fructus Gardenia),so as to evaluate quality of Zhizi pieces effectively and accurately.Methods Using established HPLC-PDA method to characterize the quality of Zhizi characteristic chromatogram of medicinal system.The quality of 16 batches of Zhizi pieces was represented according to the number and chemical type of characteristic peaks on chromatogram,and the quantity of 16 batches of Zhizi pieces was represented based on the con-tent of characteristic index components of gardenoside,genipin 1-gentiobioside,chlorogenic acid,iri-doids (represented by gardenoside)and phenylpropionic acids (represented by chlorogenic acid).The content of total terpenoids was determined by using vanillin-perchloric acid colorimetric method and the content of total phenols was detected by using chloride-potassium ferricyanide colorimetric method.Corre-lation analysis of quality and quantity representations was then performed referring to the benchmark pieces.Results As the reference substance,characteristic spectrum of Lot.6 contains nine characteristic peaks including four peaks of iridoids and five peaks of phenylpropionic acids,which all appeard on the chromatograms of 16 batches Zhizi pieces.Lot.3,4,7,8,13 Zhizi pieces had more effective index components than that of Lot.6,and Lot.7,1,9,4,3,12,5,14 pieces had higher correlation degree to Lot.6.After comprehensive evaluation,the quality of Lot.3,4,6,7 were better.Conclusion The established HPLC methods by quality and quantity representation of characteristic spectrum of 16 batches of Zhizi pieces is simple and accurate.And the evaluation mode of quantity and quality and their correla-tion based on Zhizi medicinal system,concerning on systemic correlation and application validity,can e-valuate the quality of Zhizi pieces effectively and accurately.