CAJ | 학술논문

目的：观察肿瘤坏死因子相关凋亡诱导配体（ TRAIL）对非小细胞肺癌小鼠模型肿瘤细胞凋亡诱导的作用。方法将小鼠非小细胞癌细胞株Tc-1于清洁小鼠右上肢皮下接种，共接种30只，每只接种浓度为5×106个Tc-1细胞。将上述小鼠按随机数字表法分为6组（空白对照组和五种浓度rhTRAIL阳性实验组），各5只。空白对照组每天注射一次磷酸盐溶液，阳性对照组每天注射不同浓度的重组人TRAIL（ rhTRAIL）溶液，各组均连续注射15 d。对上述30只小鼠每2日测量肿瘤直径计算肿瘤长短直径，计算肿瘤体积。取小鼠脾脏细胞测定肿瘤坏死因子（ TNF）α、干扰素（ IFN）α水平。比较上述6组小鼠肿瘤体积、抑瘤率以及TNF-α、IFN-α水平。结果五组阳性对照组小鼠肿瘤体积明显低于空白对照组[（446±17） mm3，（378±15） mm3，（333±14） mm3，（270±13） mm3，（222±11） mm3比（532±22） mm3，P＜0.05]；5组阳性对照组随着rhTRAIL浓度的增加，肿瘤体积相应减小；五组阳性组小鼠的Tc-1细胞凋亡率随着rhTRAIL浓度增高，其凋亡率逐渐升高；5组阳性组小鼠的TNF-α、IFN-α水平均明显高于空白对照组[（647±26） ng／L、（769±34） ng／L、（837±44） ng／L、（873±46） ng／L、（925±50） ng／L比（439±20） ng／L，P＜0.05；（635±25） ng／L、（780±38） ng／L、（911±50） ng／L、（965±47） ng／L、（980±43） ng／L比（385±20） ng／L，P＜0.05]。结论rhTRAIL能有效增强非小细胞肺癌小鼠体内的分泌细胞因子，有效诱导非小细胞肺癌小鼠的肿瘤细胞凋亡，说明rhTRAIL对非小细胞肺癌小鼠的肿瘤细胞具有一定的抑制作用。
목적：관찰종류배사인자상관조망유도배체（ TRAIL）대비소세포폐암소서모형종류세포조망유도적작용。방법장소서비소세포암세포주Tc-1우청길소서우상지피하접충，공접충30지，매지접충농도위5×106개Tc-1세포。장상술소서안수궤수자표법분위6조（공백대조조화오충농도rhTRAIL양성실험조），각5지。공백대조조매천주사일차린산염용액，양성대조조매천주사불동농도적중조인TRAIL（ rhTRAIL）용액，각조균련속주사15 d。대상술30지소서매2일측량종류직경계산종류장단직경，계산종류체적。취소서비장세포측정종류배사인자（ TNF）α、간우소（ IFN）α수평。비교상술6조소서종류체적、억류솔이급TNF-α、IFN-α수평。결과오조양성대조조소서종류체적명현저우공백대조조[（446±17） mm3，（378±15） mm3，（333±14） mm3，（270±13） mm3，（222±11） mm3비（532±22） mm3，P＜0.05]；5조양성대조조수착rhTRAIL농도적증가，종류체적상응감소；오조양성조소서적Tc-1세포조망솔수착rhTRAIL농도증고，기조망솔축점승고；5조양성조소서적TNF-α、IFN-α수평균명현고우공백대조조[（647±26） ng／L、（769±34） ng／L、（837±44） ng／L、（873±46） ng／L、（925±50） ng／L비（439±20） ng／L，P＜0.05；（635±25） ng／L、（780±38） ng／L、（911±50） ng／L、（965±47） ng／L、（980±43） ng／L비（385±20） ng／L，P＜0.05]。결론rhTRAIL능유효증강비소세포폐암소서체내적분비세포인자，유효유도비소세포폐암소서적종류세포조망，설명rhTRAIL대비소세포폐암소서적종류세포구유일정적억제작용。
Objective To observe the tumor cell inducing effect of tumor necrosis factor-related apopto-sis-inducing ligand ( TRAIL) in non-small cell lung cancer mouse models.Methods Non-small cell carci-noma cell line Tc-1 were inoculated subcutaneously in the right upper limb of 30 mice,5 ×106 Tc-1 cells each inoculum.The above mice were randomly divided into six groups(control group,and rhTRAIL positive groups of five concentrations),five in each group.The blank control group was injected phosphate solution once every day,the positive control groups were injected rhTRAIL solution of different concentrations continu-ously for 15 d.Tumor diameter of the above mice were measured and recorded every 2 d, and the tumor volumes were calculated.Tumor necrosis factor α(TNF-α),interferon α(IFN-α) concentrations from the spleen cells of the mice were measured.The tumor volume,tumor inhibition rate,and TNF-α,IFN-αconcen-trations of the six groups were compared.Results The tumor volume of the five positive groups were statisti-cally significantly lower than the blank control group [ ( 446 ±17 ) mm3 , ( 378 ±15 ) mm3 , ( 333 ± 14) mm3,(270 ±13) mm3,(222 ±11) mm3 vs (532 ±22) mm3,P <0.05];with the increase of rhTRAIL concentration,the tumor volume of the five positive groups reduced accordingly;Tc 1 cell apoptosis rate in mice increased with the increase of rhTRAIL concentration; TNF-α,IFN-αconcentration in mice of the five positive groups were significantly higher than the blank control group [ ( 647 ±26 ) ng/L, ( 769 ± 34) ng/L, (837 ±44) ng/L,(873 ±46) ng/L,(925 ±50) ng/L vs (439 ±20) ng/L,P<0.05;(635 ± 25) ng/L,(780 ±38)ng/L,(911 ±50) ng/L,(965 ±47) ng/L,(980 ±43) ng/L vs (385 ±20) ng/L, P<0.05].Conclusion rhTRAIL can effectively enhance cytokines secretion of the non-small cell lung cancer in mice,induce apoptosis of the tumor cells,indicating rhTRAIL has a certain inhibitory effect for non-small cell lung cancer cells in mice.