检验医学与临床
檢驗醫學與臨床
검험의학여림상
Laboratory Medicine and Clinic
2015年
18期
2690-2693
,共4页
大环内酯类%抗菌活性%细菌耐药性%pH 值
大環內酯類%抗菌活性%細菌耐藥性%pH 值
대배내지류%항균활성%세균내약성%pH 치
macrolides%antibacterial activity%bacterial resistance%pH values
目的：不同 pH 值条件下3种大环内酯类药物对金黄色葡萄球菌和化脓性链球菌的耐药性和最小抑菌浓度（MIC）。方法在 pH6．0、pH7．0和 pH8．0条件下，采用肉汤稀释法分别检测大环内酯类抗菌药物对临床分离金黄色葡萄球菌和化脓性链球菌 MIC 及细菌耐药性。结果随着 pH 值的增加，大环内酯类抗菌药物对金黄色葡萄球菌和化脓性链球菌的 MIC 值均有不同程度的降低，并呈一定的依赖效应，其中罗红霉素和阿奇霉素的抑菌活性强于红霉素；体外诱导试验表明，在所有试验 pH 值条件下，MIC 值均有所增加，但是随着 pH 值的增加细菌的 MIC 值逐渐降低。结论大环内酯类抗菌药物的 MIC 随培养基 pH 值升高而逐渐降低，在一定 pH 值范围内大环内酯类抗菌药物可提高金黄色葡萄球菌和化脓性链球菌的抗菌作用。
目的：不同 pH 值條件下3種大環內酯類藥物對金黃色葡萄毬菌和化膿性鏈毬菌的耐藥性和最小抑菌濃度（MIC）。方法在 pH6．0、pH7．0和 pH8．0條件下，採用肉湯稀釋法分彆檢測大環內酯類抗菌藥物對臨床分離金黃色葡萄毬菌和化膿性鏈毬菌 MIC 及細菌耐藥性。結果隨著 pH 值的增加，大環內酯類抗菌藥物對金黃色葡萄毬菌和化膿性鏈毬菌的 MIC 值均有不同程度的降低，併呈一定的依賴效應，其中囉紅黴素和阿奇黴素的抑菌活性彊于紅黴素；體外誘導試驗錶明，在所有試驗 pH 值條件下，MIC 值均有所增加，但是隨著 pH 值的增加細菌的 MIC 值逐漸降低。結論大環內酯類抗菌藥物的 MIC 隨培養基 pH 值升高而逐漸降低，在一定 pH 值範圍內大環內酯類抗菌藥物可提高金黃色葡萄毬菌和化膿性鏈毬菌的抗菌作用。
목적：불동 pH 치조건하3충대배내지류약물대금황색포도구균화화농성련구균적내약성화최소억균농도（MIC）。방법재 pH6．0、pH7．0화 pH8．0조건하，채용육탕희석법분별검측대배내지류항균약물대림상분리금황색포도구균화화농성련구균 MIC 급세균내약성。결과수착 pH 치적증가，대배내지류항균약물대금황색포도구균화화농성련구균적 MIC 치균유불동정도적강저，병정일정적의뢰효응，기중라홍매소화아기매소적억균활성강우홍매소；체외유도시험표명，재소유시험 pH 치조건하，MIC 치균유소증가，단시수착 pH 치적증가세균적 MIC 치축점강저。결론대배내지류항균약물적 MIC 수배양기 pH 치승고이축점강저，재일정 pH 치범위내대배내지류항균약물가제고금황색포도구균화화농성련구균적항균작용。
Objective To investigate the effect of staphylococcus aureus and streptococcus pyogenes on the drug resistance and the minimal inhibition concentration (MIC) of macrolide antibiotics under the different pH value conditions .Methods The broth dilution method was used to detect the drug resistance and MIC of macrolide antibi‐otics to clinically isolated staphylococcus aureus and streptococcus pyogenes .Results The drug resistance and MIC of macrolide antibiotics to staphylococcus aureus and streptococcus pyogenes were decreased with enhance of pH val‐ue in certainly dose‐dependent manner .In addition ,the inhibition activity of roxithromycin and azithromycin were stronger than that of erythromycin .The in vitro study indicated that the MIC values were increased in all the experi‐ment pH conditions ,but the MIC values gradually were decreased with augment of pH values .Conclusion The MIC values of macrolide antibiotics are gradually decreased with augment of pH values in culture medium ,and macrolide antibiotics can improve the antibacterial activity of staphylococcus aureus and streptococcus pyogenes in certain pH values condition .
