CAJ | 학술논문

目的：研究槲皮素对 SW480结肠癌细胞生长增殖及蛋白表达的影响。方法体外培养人结肠癌SW480细胞后，采用人工重组基膜技术监测槲皮素对肿瘤细胞体外侵袭能力和运动活性的作用。将培养成功的细胞分为空白组和其余5个不同浓度（10、20、40、80、160 mg／L）的槲皮素作用组，观察干预结果，检测人结肠癌 SW480细胞的增殖抑制率，选择最佳作用浓度；并通过免疫印迹法检测人结肠癌SW480内增殖细胞核抗原（ PCNA）、Ki67的表达情况。结果结肠癌 SW480细胞经槲皮素处理后，体外侵袭、运动能力呈剂量依赖性下降（ P ＜0.05）；槲皮素能显著抑制结肠癌SW480细胞的增殖，浓度为10、20、40、80、160μmol／L的槲皮素作用于结肠癌 SW480细胞的增殖抑制率分别为（4.1±1.2）％、（13.9±1.5）％、（38.8±2.2）％、（41.5±2.6）％、（45.3±2.6）％，与空白组对照相比差异有统计学意义（ P＜0.05）；干预的结肠癌 SW480细胞内 Ki67和 PCNA的表达均呈下调表现（P＜0.05）。结论槲皮素可呈浓度和时间依赖性地抑制结肠癌SW480细胞的增殖，对人结肠癌 SW480细胞的生长有抑制作用，并能诱导其凋亡。
목적：연구곡피소대 SW480결장암세포생장증식급단백표체적영향。방법체외배양인결장암SW480세포후，채용인공중조기막기술감측곡피소대종류세포체외침습능력화운동활성적작용。장배양성공적세포분위공백조화기여5개불동농도（10、20、40、80、160 mg／L）적곡피소작용조，관찰간예결과，검측인결장암 SW480세포적증식억제솔，선택최가작용농도；병통과면역인적법검측인결장암SW480내증식세포핵항원（ PCNA）、Ki67적표체정황。결과결장암 SW480세포경곡피소처리후，체외침습、운동능력정제량의뢰성하강（ P ＜0.05）；곡피소능현저억제결장암SW480세포적증식，농도위10、20、40、80、160μmol／L적곡피소작용우결장암 SW480세포적증식억제솔분별위（4.1±1.2）％、（13.9±1.5）％、（38.8±2.2）％、（41.5±2.6）％、（45.3±2.6）％，여공백조대조상비차이유통계학의의（ P＜0.05）；간예적결장암 SW480세포내 Ki67화 PCNA적표체균정하조표현（P＜0.05）。결론곡피소가정농도화시간의뢰성지억제결장암SW480세포적증식，대인결장암 SW480세포적생장유억제작용，병능유도기조망。
Objective To study the influence of quercetin on the proliferation and protein expression SW480 colon cancer cell.Methods Human SW480 colon cancer cells were cultured in vitro,then artificial recombination of the basement membrane was adopted to observe quercetin effect on cell invasion and motor activity.The successfully cultured cells were divided into blank group and the rest into five quercetin groups of different concentrations of quercetin (10,20,40,80,160 mg/L),then human colon cancer SW480 cells proliferation inhibition rates were tested , based on which the best concentration was cosen; Western blot method was used to detect the expression of cell nucleus antigen (PCNA and Ki67) in human SW480 colon cancer.Results After treatment by quercetin, the in vitro invasion, motor ability of SW480 colon cancer cells presented dose-dependent decline(P<0.05);different concentrations(10,20,40,80,160 μmol/L) of quercetin and its proliferation inhibition rates were ( 4.1 ±1.2 )%, ( 13.9 ±1.5 )%, ( 38.8 ±2.2 )%, (41.5 ±2.6)%,(45.3 ±2.6)%,the differences from the blank control were statistically significant ( P<0.05 ); the expression of Ki67 and PCNA in SW480 colon cancer cells after intervention showed down trend (P<0.05).Conclusion Quercetin can inhibit the proliferation of SW480 colon cancer cells in a concen-tration and time dependent way, which has inhibitory effect on the growth of human SW480 colon cancer cells,and can induce its apoptosis.