中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
Chinese Journal of Anesthesiology
2015年
6期
700-703
,共4页
异氟醚%脂肪乳剂,静脉注射用%缺血预处理%再灌注损伤%肺
異氟醚%脂肪乳劑,靜脈註射用%缺血預處理%再灌註損傷%肺
이불미%지방유제,정맥주사용%결혈예처리%재관주손상%폐
Isoflurane%Fat emulsions,intravenous%Ischemic preconditioning%Reperfusion injury%Lung
目的 探讨乳化异氟醚预处理对大鼠肺缺血再灌注损伤的影响.方法 雄性SD大鼠32只,体重250～ 320 g,采用随机数字表法分成4组(n=8):假手术组(S组)、肺缺血再灌注损伤组(Ⅰ/R组)、脂肪乳组(IL组)和乳化异氟醚预处理组(El组).采用阻断左肺门lh再灌注2h的方法制备肺缺血再灌注损伤模型.IL组腹腔注射30％脂肪乳10.5 ml/kg,EI组腹腔注射8％乳化异氟醚,24 h后制备模型.于再灌注2h时,取血样,测定PaO2和PaCO2,采用硫代巴比妥酸法测定血浆MDA浓度.处死大鼠,取左肺组织,光镜下观察肺组织病理学结果,计算湿重/干重(W/D)比值,采用比色法测定髓过氧化物酶(MPO)活性.结果 与S组比较,其余3组肺组织W/D比值、MPO活性、血浆MDA浓度和PaCO2升高,PaO2降低(P＜0.05);与Ⅰ/R组比较,IL组和EI组肺组织W/D比值、MPO活性、血浆MDA浓度和PaCO2降低,PaO2升高(P＜0.05);与IL组比较,EI组肺组织W/D比值、MPO活性、血浆MDA浓度和PaCO2降低,PaO2升高(P＜0.05).EI组肺病理学损伤较Ⅰ/R组明显减轻.结论 乳化异氟醚预处理可减轻大鼠肺缺血再灌注损伤,其机制与抑制肺组织炎性反应和氧自由基生成有关.
目的 探討乳化異氟醚預處理對大鼠肺缺血再灌註損傷的影響.方法 雄性SD大鼠32隻,體重250～ 320 g,採用隨機數字錶法分成4組(n=8):假手術組(S組)、肺缺血再灌註損傷組(Ⅰ/R組)、脂肪乳組(IL組)和乳化異氟醚預處理組(El組).採用阻斷左肺門lh再灌註2h的方法製備肺缺血再灌註損傷模型.IL組腹腔註射30％脂肪乳10.5 ml/kg,EI組腹腔註射8％乳化異氟醚,24 h後製備模型.于再灌註2h時,取血樣,測定PaO2和PaCO2,採用硫代巴比妥痠法測定血漿MDA濃度.處死大鼠,取左肺組織,光鏡下觀察肺組織病理學結果,計算濕重/榦重(W/D)比值,採用比色法測定髓過氧化物酶(MPO)活性.結果 與S組比較,其餘3組肺組織W/D比值、MPO活性、血漿MDA濃度和PaCO2升高,PaO2降低(P＜0.05);與Ⅰ/R組比較,IL組和EI組肺組織W/D比值、MPO活性、血漿MDA濃度和PaCO2降低,PaO2升高(P＜0.05);與IL組比較,EI組肺組織W/D比值、MPO活性、血漿MDA濃度和PaCO2降低,PaO2升高(P＜0.05).EI組肺病理學損傷較Ⅰ/R組明顯減輕.結論 乳化異氟醚預處理可減輕大鼠肺缺血再灌註損傷,其機製與抑製肺組織炎性反應和氧自由基生成有關.
목적 탐토유화이불미예처리대대서폐결혈재관주손상적영향.방법 웅성SD대서32지,체중250～ 320 g,채용수궤수자표법분성4조(n=8):가수술조(S조)、폐결혈재관주손상조(Ⅰ/R조)、지방유조(IL조)화유화이불미예처리조(El조).채용조단좌폐문lh재관주2h적방법제비폐결혈재관주손상모형.IL조복강주사30％지방유10.5 ml/kg,EI조복강주사8％유화이불미,24 h후제비모형.우재관주2h시,취혈양,측정PaO2화PaCO2,채용류대파비타산법측정혈장MDA농도.처사대서,취좌폐조직,광경하관찰폐조직병이학결과,계산습중/간중(W/D)비치,채용비색법측정수과양화물매(MPO)활성.결과 여S조비교,기여3조폐조직W/D비치、MPO활성、혈장MDA농도화PaCO2승고,PaO2강저(P＜0.05);여Ⅰ/R조비교,IL조화EI조폐조직W/D비치、MPO활성、혈장MDA농도화PaCO2강저,PaO2승고(P＜0.05);여IL조비교,EI조폐조직W/D비치、MPO활성、혈장MDA농도화PaCO2강저,PaO2승고(P＜0.05).EI조폐병이학손상교Ⅰ/R조명현감경.결론 유화이불미예처리가감경대서폐결혈재관주손상,기궤제여억제폐조직염성반응화양자유기생성유관.
Objective To investigate the effects of emulsified isoflurane preconditioning on lung ischemia-reperfusion (Ⅰ/R) injury in rats.Methods Thirty-two male Sprague-Dawley rats,weighing 250-320 g,were randomly divided into 4 groups (n =8 each) using a random number table:sham operation group (group S),group Ⅰ/R,intralipid group (group IL),and emulsified isoflurane preconditioning group (group EI).Lung Ⅰ/R was produced by clamping the left hilum of lungs for 1 h followed by 2 h of reperfusion in anesthetized rats.In group S,intraperitoneal normal saline 10.5 ml/kg was injected,but the hilum was only exposed 24 h later.In groups Ⅰ/R,IL and EI,intraperitoneal normal saline 10.5 ml/kg,30％ intralipid 10.5 ml/kg and 8％ emulsified isoflurane 10.5 ml/kg were injected intraperitoneally,respectively,and then the model was established 24 h later.At the end of reperfusion,blood samples were collected from the left ventricle for measurement of PaO2,PaCO2,plasma malondialdehyde (MDA) concentration (by ELISA).The rats were then sacrificed,and left lungs were removed immediately for microscopic examination and for determination of myleoperoxidase (MPO) activity (using colorimetric method) in pulmonary specimens.Wet/dry lung weight ratio (W/D ratio) was calculated.Results Compared with group S,W/D ratio,MPO activity,plasma MDA concentration and PaCO2 were significantly increased,and PaO2 was decreased in the other three groups.Compared with group Ⅰ/R,W/D ratio,MPO activity,plasma MDA concentration and PaCO2 were significantly decreased,and PaO2 was increased in IL and EI groups.W/D ratio,MPO activity,plasma MDA concentration and PaCO2 were significantly lower,and PaO2 was higher in group EI than in group IL.The pathological changes of the lung were significantly attenuated in group EI compared with group Ⅰ/R.Conclusion Emulsified isoflurane preconditioning can reduce lung Ⅰ/R injury in rats,and inhibition of pulmonary inflammatory responses and of production of oxygen free radicals are involved in the mechanism.
