中国妇幼健康研究
中國婦幼健康研究
중국부유건강연구
Chinese Journal of Woman and Child Health Research
2015年
4期
706-709
,共4页
张燕%滕月%张键%李婧%李旭
張燕%滕月%張鍵%李婧%李旭
장연%등월%장건%리청%리욱
卵巢癌%神经纤毛蛋白1%慢病毒%细胞增殖
卵巢癌%神經纖毛蛋白1%慢病毒%細胞增殖
란소암%신경섬모단백1%만병독%세포증식
ovarian cancer%neuropilin 1(Nrp1)%lentivirus%cell proliferation
目的 研究神经纤毛蛋白1 (neuropilin 1,Nrp1)在卵巢癌中的作用,探讨其作为治疗靶点的可行性. 方法 克隆Nrp1基因,采用慢病毒包装体系包装过表达Nrp1的重组慢病毒,感染卵巢癌SKOV3细胞,嘌呤霉素筛选稳定高表达Nrp1的SKOV3细胞,通过细胞计数检测其增殖能力的改变. 结果 成功包装了过表达Nrp1重组慢病毒,筛选得到了稳定高表达Nrp1的SKOV3细胞,其增殖能力显著高于对照细胞(t>t0.05(4) ,P<0.05). 结论 Nrp1促进卵巢癌SKOV3细胞增殖,可能在卵巢癌发生发展过程中起促进作用.
目的 研究神經纖毛蛋白1 (neuropilin 1,Nrp1)在卵巢癌中的作用,探討其作為治療靶點的可行性. 方法 剋隆Nrp1基因,採用慢病毒包裝體繫包裝過錶達Nrp1的重組慢病毒,感染卵巢癌SKOV3細胞,嘌呤黴素篩選穩定高錶達Nrp1的SKOV3細胞,通過細胞計數檢測其增殖能力的改變. 結果 成功包裝瞭過錶達Nrp1重組慢病毒,篩選得到瞭穩定高錶達Nrp1的SKOV3細胞,其增殖能力顯著高于對照細胞(t>t0.05(4) ,P<0.05). 結論 Nrp1促進卵巢癌SKOV3細胞增殖,可能在卵巢癌髮生髮展過程中起促進作用.
목적 연구신경섬모단백1 (neuropilin 1,Nrp1)재란소암중적작용,탐토기작위치료파점적가행성. 방법 극륭Nrp1기인,채용만병독포장체계포장과표체Nrp1적중조만병독,감염란소암SKOV3세포,표령매소사선은정고표체Nrp1적SKOV3세포,통과세포계수검측기증식능력적개변. 결과 성공포장료과표체Nrp1중조만병독,사선득도료은정고표체Nrp1적SKOV3세포,기증식능력현저고우대조세포(t>t0.05(4) ,P<0.05). 결론 Nrp1촉진란소암SKOV3세포증식,가능재란소암발생발전과정중기촉진작용.
Objective To study the function of neuropilin 1 (Nrp1) in ovarian cancer and the possibility of Nrp1 as therapeutic target. Methods Nrp 1 gene was cloned, and Nrp 1 overexpressing recombinant lentivirus was packed.After infection of SKOV3 ovarian cancer cells, puromycin selection generated the stable cell clones overexpressing Nrp 1.Cell number counting assay was performed to detect the cell proliferation. Results Nrp 1 overexpressing recombinant lentivirus was successfully packed. The stable SKOV3 cell clones overexpressing Nrp 1 was selected, and it had more powerful proliferation ability than control cells (t>t0.05(4), P<0.05).Conclusion Nrp1 promotes the proliferation of SKOV3 cells, and may stimulate the progression of ovarian cancer.
