CAJ | 학술논문

目的：研究外周血 T 细胞亚群及 IL‐17、IL‐10在原发性干燥综合征（pSS）患者中的表达。方法选取该院风湿科门诊或住院诊断的 pSS 患者89例，健康对照组选自该院健康体检者38例，用流式细胞仪检测外周血T 细胞亚群及 ELISA 检验血清中的 IL‐17、IL‐10表达水平。结果外周血 T 细胞亚群检测结果显示，pSS 患者CD3＋与健康人比较差异无统计学意义（P＞0．05）；CD4＋较健康人明显下降（P＜0．05）；CD8＋较健康人极明显升高（P＜0．05）；CD4＋／CD8＋较健康人极明显下降（P＜0．05）；细胞因子检测结果显示，IL‐17水平较健康人明显升高（P＜0．05）；IL‐10水平较健康人极明显降低（P＜0．05）。结论 pSS 患者较健康人存在明显的 T 细胞免疫异常，患者自身存在炎性反应及缺乏对炎性反应的抑制功能。
목적：연구외주혈 T 세포아군급 IL‐17、IL‐10재원발성간조종합정（pSS）환자중적표체。방법선취해원풍습과문진혹주원진단적 pSS 환자89례，건강대조조선자해원건강체검자38례，용류식세포의검측외주혈T 세포아군급 ELISA 검험혈청중적 IL‐17、IL‐10표체수평。결과외주혈 T 세포아군검측결과현시，pSS 환자CD3＋여건강인비교차이무통계학의의（P＞0．05）；CD4＋교건강인명현하강（P＜0．05）；CD8＋교건강인겁명현승고（P＜0．05）；CD4＋／CD8＋교건강인겁명현하강（P＜0．05）；세포인자검측결과현시，IL‐17수평교건강인명현승고（P＜0．05）；IL‐10수평교건강인겁명현강저（P＜0．05）。결론 pSS 환자교건강인존재명현적 T 세포면역이상，환자자신존재염성반응급결핍대염성반응적억제공능。
Objective The research the expression of peripheral blood T‐lymphocyte subsets ,IL‐17 and IL‐10 in primary Sjogren′s Syndrome(pSS) .Methods 89 patients with pSS diagnosed in the outpatient department of rheu‐matic disease or the inpatient department of our hospital were selected .And 38 healthy persons undergoing the physi‐cal examination were selected as the control group .T‐lymphocyte subsets were identified and classified by the flow cytometer .The expression levels of IL‐17 and IL‐10 in serum were detected by ELISA .Results The results of pe‐ripheral blood T‐lymphocyte subsets showed that CD3 + had no statistical differences between healthy people and pSS patients(P> 0 .05) ,the CD4 + level in the pSS patients was obviously lower than that in the normal persons (P<0 .05) ,while CD8 + was extremely increased compared with the normal healthy persons(P< 0 .05) and CD4 + /CD8 +was extremely decreased compared with the normal healthy persons (P< 0 .05) .The results of cytokines showed that the level of IL‐17 was significantly increased compared with the healthy persons (P< 0 .05) and the IL‐10 level ex‐tremely decreased compared with the normal healthy persons ( P < 0 .05) .Conclusion Compared with the healthy persons ,the obvious immune abnormality of T‐lymphocyte exists in the pSS patients ,the patients themselves exist the inflammatory reaction and lack the inhibitory ability to inflammatory reaction .