CAJ | 학술논문

本试验旨在研究不同粗蛋白质( CP)水平饲粮添加外源蛋白酶对肉鸡生产性能、血清生化指标和抗氧化功能的影响. 采用3 × 3 双因素试验设计,即3 种 CP 水平,分别为正对照(PC,CP水平1~21 d为21%,22~42 d为19%)、负对照1(NC1,CP水平在PC基础上降1%)和负对照2( NC2,CP水平在 PC 基础上降2%);3 种蛋白酶添加水平( 0、150 和300 mg/kg).选取1 080只1日龄爱拔益加( AA)肉鸡,随机分为9个处理,每个处理6个重复,每个重复20只鸡,公母各占1/2. 试验期42 d. 结果表明:1) CP水平对肉鸡平均体重( ABW)、平均日增重(ADG)、平均日采食量(ADFI)和料重比(F/G)有显著影响(P<0.05). 当CP水平从PC降至NC2时,ABW和ADG显著降低( P<0.05) ,F/G显著增加( P<0.05);蛋白酶水平对F/G有显著影响(P<0.05),300 mg/kg蛋白酶处理显著降低了肉鸡1~21 d的F/G(P<0.05). 2)CP水平对血清白蛋白和球蛋白含量有显著影响( P<0.05) ,PC处理42 d血清白蛋白含量显著高于NC2处理(P<0.05),血清球蛋白含量显著高于NC1处理(P<0.05);蛋白酶水平对血清肌酐含量有显著影响( P<0.05) ,300 mg/kg蛋白酶处理21 d血清肌酐含量显著降低( P<0.05);蛋白酶水平和CP水平的互作对21 d血清总蛋白和球蛋白含量有显著影响( P<0.05). 3) CP水平对血清中谷胱甘肽过氧化物酶( GSH-Px)活性有显著影响( P<0.05) ,PC处理42 d血清GSH-Px活性显著高于NC2处理(P<0.05);蛋白酶水平对血清GSH-Px活性和丙二醛(MDA)含量有显著影响( P<0.05) ,与不加酶处理相比,300 mg/kg 蛋白酶处理21 d 血清 GSH-Px 活性显著升高( P<0.05) ,血清MDA含量显著降低( P<0.05). 由此可见,饲粮CP水平降低1%后添加300 mg/kg蛋白酶对肉鸡生产性能、血清生化指标和抗氧化功能无影响,在基础饲粮中直接添加300 mg/kg蛋白酶效果更佳.
본시험지재연구불동조단백질( CP)수평사량첨가외원단백매대육계생산성능、혈청생화지표화항양화공능적영향. 채용3 × 3 쌍인소시험설계,즉3 충 CP 수평,분별위정대조(PC,CP수평1~21 d위21%,22~42 d위19%)、부대조1(NC1,CP수평재PC기출상강1%)화부대조2( NC2,CP수평재 PC 기출상강2%);3 충단백매첨가수평( 0、150 화300 mg/kg).선취1 080지1일령애발익가( AA)육계,수궤분위9개처리,매개처리6개중복,매개중복20지계,공모각점1/2. 시험기42 d. 결과표명:1) CP수평대육계평균체중( ABW)、평균일증중(ADG)、평균일채식량(ADFI)화료중비(F/G)유현저영향(P<0.05). 당CP수평종PC강지NC2시,ABW화ADG현저강저( P<0.05) ,F/G현저증가( P<0.05);단백매수평대F/G유현저영향(P<0.05),300 mg/kg단백매처리현저강저료육계1~21 d적F/G(P<0.05). 2)CP수평대혈청백단백화구단백함량유현저영향( P<0.05) ,PC처리42 d혈청백단백함량현저고우NC2처리(P<0.05),혈청구단백함량현저고우NC1처리(P<0.05);단백매수평대혈청기항함량유현저영향( P<0.05) ,300 mg/kg단백매처리21 d혈청기항함량현저강저( P<0.05);단백매수평화CP수평적호작대21 d혈청총단백화구단백함량유현저영향( P<0.05). 3) CP수평대혈청중곡광감태과양화물매( GSH-Px)활성유현저영향( P<0.05) ,PC처리42 d혈청GSH-Px활성현저고우NC2처리(P<0.05);단백매수평대혈청GSH-Px활성화병이철(MDA)함량유현저영향( P<0.05) ,여불가매처리상비,300 mg/kg 단백매처리21 d 혈청 GSH-Px 활성현저승고( P<0.05) ,혈청MDA함량현저강저( P<0.05). 유차가견,사량CP수평강저1%후첨가300 mg/kg단백매대육계생산성능、혈청생화지표화항양화공능무영향,재기출사량중직접첨가300 mg/kg단백매효과경가.
The experiment was conducted to investigate the effects of different crude protein ( CP) levels diet supplemented with exogenous protease on performance, serum biochemical indices and antioxidant function of broilers. A 3×3 factorial design with 3 CP levels (21%, 20% and 19% at 1 to 21 days, 19%, 18% and 17%at 22 to 42 days) and 3 protease levels (0, 150 and 300 mg/kg). High CP diet without protease was a positive control ( PC) treatment, low CP diets were two negative control ( NC1 and NC2) treatments. A total of 1 080 one-day-old Arbor Acres ( AA) broilers were randomly assigned to 9 treatments with 6 replicates per treatment and 20 broilers per replicate ( half male and half female ) . The experiment lasted for 42 days. The results showed as follows: 1 ) CP levels had significant effects on feed-to-gain ratio ( F/G ) , average body weight ( ABW) , average daily gain ( ADG) and average daily feed intake ( ADFI) of broilers ( P<0.05) , when the dietary CP levels was reduced from PC to NC2, ABW and ADG were significantly reduced ( P<0.05) , F/G was significantly increased (P<0.05). Protease level had significant effects on F/G (P<0.05), 300 mg/kg protease supplementation significantly reduced F/G of broilers at 1 to 21 days. 2 ) CP levels had significantly effects on the content of albumin and globulin in serum ( P<0.05) . The serum albumin content of broilers at 42 days in PC treatment was significantly higher than that in NC2 treatment (P<0.05), and the serum globulin content was significantly higher than that of NC1 treatment ( P<0.05) . Protease level had significant effects on serum creatinine content (P<0.05), 300 mg/kg protease supplementation significantly reduced serum creati-nine content of broilers at 21 days ( P<0.05) . The interaction between protease level and CP level had signifi-cantly effects on the content of total protein and globulin in serum of broilers at 21 days ( P<0.05) . 3) CP lev-els had significantly effect on serum glutathione peroxidase ( GSH-Px) activity ( P<0.05) , the serum GSH-Px activity of broilers at 42 days in PC treatment was significantly higher than that in NC2 treatment ( P<0.05) , protease level had significantly effect on serum GSH-Px activity and malondialdehyde ( MDA) content; com-pared with the group without enzymes, serum GSH-Px activity was increased significantly and serum MDA content was decreased significantly in 300 mg/kg protease supplementation treatment (P<0.05). In conclu-sion, when dietary CP levels is reduced 1%, 300 mg/kg protease supplementation has no effects on perform-ance, serum biochemical indices and antioxidant function of broilers, and 300 mg/kg protease supplementation in basal diets has a better effect.