CAJ | 학술논문

目的：分析2014年杭州地区体检人群乙型肝炎病毒血清学五项标志物（HBV‐M ）全阴模式的分布特征，为预防和控制HBV‐M阴性人群感染乙型肝炎病毒（HBV ）提供对策。方法采用 ELISA 对体检人群的血液标本进行 HBV‐M （HBsAg、HBsAb、HBeAg、HBeAb和HBcAb）检测。对保存的300例 HBV‐M阴性标本，分别使用化学发光免疫分析法检测 HBsAg、HB‐sAb和PROCLEIX ULTRIO棆 Assay检测HBV‐DNA ，对 HBV‐DNA反应性标本进行病毒载量测定。结果在检测的9143例体检者的标本中，2213例标本为 HBV‐M阴性，阴性率为24．20％；男女 HBV‐M 阴性率比值为1∶1．21。使用化学发光免疫分析法和PROCLEIX ULTRIO棆 Assay分别检测出1例低浓度的HBsAg标本（HBsAb及 HBV DNA均无反应性），4例低浓度的HBsAb标本（HBsAg及 HBV DNA均无反应性），2例 HBV‐DNA反应性标本（HBV‐M 阴性）。其中1例 HBV‐DNA反应性标本定量检测为560 IU／mL。结论杭州地区ELISA HBV‐M 阴性的体检人群中，少数人可检出低浓度的 HBsAg或 HBsAb或HBV DNA。针对ELISA HBV‐M阴性人群，建议接种乙肝疫苗前进行HBV‐M和HBV‐DNA定量检测，以确定是否需要接种乙肝疫苗及接种乙肝疫苗的方案。
목적：분석2014년항주지구체검인군을형간염병독혈청학오항표지물（HBV‐M ）전음모식적분포특정，위예방화공제HBV‐M음성인군감염을형간염병독（HBV ）제공대책。방법채용 ELISA 대체검인군적혈액표본진행 HBV‐M （HBsAg、HBsAb、HBeAg、HBeAb화HBcAb）검측。대보존적300례 HBV‐M음성표본，분별사용화학발광면역분석법검측 HBsAg、HB‐sAb화PROCLEIX ULTRIO륜 Assay검측HBV‐DNA ，대 HBV‐DNA반응성표본진행병독재량측정。결과재검측적9143례체검자적표본중，2213례표본위 HBV‐M음성，음성솔위24．20％；남녀 HBV‐M 음성솔비치위1∶1．21。사용화학발광면역분석법화PROCLEIX ULTRIO륜 Assay분별검측출1례저농도적HBsAg표본（HBsAb급 HBV DNA균무반응성），4례저농도적HBsAb표본（HBsAg급 HBV DNA균무반응성），2례 HBV‐DNA반응성표본（HBV‐M 음성）。기중1례 HBV‐DNA반응성표본정량검측위560 IU／mL。결론항주지구ELISA HBV‐M 음성적체검인군중，소수인가검출저농도적 HBsAg혹 HBsAb혹HBV DNA。침대ELISA HBV‐M음성인군，건의접충을간역묘전진행HBV‐M화HBV‐DNA정량검측，이학정시부수요접충을간역묘급접충을간역묘적방안。
Objective To analyse the distribution characteristics of negative pattern of hepatitis B virus Markers (HBV‐M ) in healthy population in Hangzhou district in 2014 ,and provide strategy for the prevention and control of HBV infection in HBV‐M negative population .Methods The HBV‐M (HBsAg ,HBsAb ,HBeAg ,HBeAb and HBcAb) in blood specimens of health examina‐tion population were tested by using ELISA .For 300 cases preserved HBV‐M negative specimens ,HBsAg and HBsAb were detec‐ted by using chemiluminescence immunoassay and HBV‐DNA was detected by using PROCLEIX ULTRIO? Assay .The viral load of HBV‐DNA reactive sample was quantitatively determined .Results Among 9 143 blood samples ,2 213 samples were HBV‐M negative ,and the negative rate was 24 .20% .The negative rate of male to female was 1∶1 .21 .Using chemiluminescence immunoas‐says and PROCLEIX ULTRIO? Assay simultaneously ,we found one case of low concentration of HBsAg(both HBsAb and HBV DNA nonreactive) ,four cases of low concentration of HBsAb(both HBsAg and HBV DNA nonreactive) ,two cases of HBV‐DNA reactive(HBV‐M negative) .One HBV‐DNA reactive sample could be quantified as 560 IU/mL .Conclusion In HBV‐M (ELISA) negative population of health examination of Hangzhou district ,a few subjects had low concentrations of HBsAg or HBsAb or HBV‐DNA .For HBV‐M negative population ,quantitative detection of HBV‐M and HBV‐DNA before HBV vaccination is recom‐mended to determine w hether they need HBV vaccine and the HBV vaccination plan .