中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
Chinese Journal of Pathophysiology
2015年
9期
1720-1723
,共4页
丹参酮IIA%高糖%内皮细胞%细胞凋亡
丹參酮IIA%高糖%內皮細胞%細胞凋亡
단삼동IIA%고당%내피세포%세포조망
Tanshinone IIA%High glucose%Endothelial cells%Apoptosis
目的：观察丹参酮IIA对高糖刺激人脐静脉内皮细胞凋亡的影响，并探讨相关的作用机制。方法：四甲基偶氮唑蓝（ MTT）法检测细胞存活率；流式细胞术Annexin V－FITC／PI双染法定量检测内皮细胞凋亡情况；免疫印迹法检测抗凋亡分子Bcl－2、促凋亡分子Bax以及细胞色素C（cytochrome C，Cyt C）的蛋白表达水平。结果：丹参酮IIA可抑制高糖诱导的人脐静脉内皮细胞存活率的减少以及凋亡率的增加。此外，丹参酮IIA处理后，Bcl－2蛋白表达显著增加，Bax蛋白表达显著减少，并且线粒体Cyt C释放受到明显抑制。结论：丹参酮IIA可以通过调节线粒体凋亡信号通路相关蛋白表达而对抗高糖诱导内皮细胞的凋亡。
目的：觀察丹參酮IIA對高糖刺激人臍靜脈內皮細胞凋亡的影響，併探討相關的作用機製。方法：四甲基偶氮唑藍（ MTT）法檢測細胞存活率；流式細胞術Annexin V－FITC／PI雙染法定量檢測內皮細胞凋亡情況；免疫印跡法檢測抗凋亡分子Bcl－2、促凋亡分子Bax以及細胞色素C（cytochrome C，Cyt C）的蛋白錶達水平。結果：丹參酮IIA可抑製高糖誘導的人臍靜脈內皮細胞存活率的減少以及凋亡率的增加。此外，丹參酮IIA處理後，Bcl－2蛋白錶達顯著增加，Bax蛋白錶達顯著減少，併且線粒體Cyt C釋放受到明顯抑製。結論：丹參酮IIA可以通過調節線粒體凋亡信號通路相關蛋白錶達而對抗高糖誘導內皮細胞的凋亡。
목적：관찰단삼동IIA대고당자격인제정맥내피세포조망적영향，병탐토상관적작용궤제。방법：사갑기우담서람（ MTT）법검측세포존활솔；류식세포술Annexin V－FITC／PI쌍염법정량검측내피세포조망정황；면역인적법검측항조망분자Bcl－2、촉조망분자Bax이급세포색소C（cytochrome C，Cyt C）적단백표체수평。결과：단삼동IIA가억제고당유도적인제정맥내피세포존활솔적감소이급조망솔적증가。차외，단삼동IIA처리후，Bcl－2단백표체현저증가，Bax단백표체현저감소，병차선립체Cyt C석방수도명현억제。결론：단삼동IIA가이통과조절선립체조망신호통로상관단백표체이대항고당유도내피세포적조망。
AIM:To investigate the effect of tanshinone IIA on the apoptosis of human umbilical vein endothe -lial cells (HUVECs) after high glucose treatment.METHODS:The cell viability was determined by MTT assay.The cell apoptotic rate was examined by flow cytometry with Annexin V-FITC/PI double staining .The expression of Bcl-2 and Bax, and the release of mitochondrial cytochrome C ( Cyt C) were analyzed by Western blotting .RESULTS: Tanshinone IIA significantly inhibited high glucose-induced decrease in cell viability and increased the cell apoptosis .Additionally, after tanshinone IIA treatment , Bax expression and the release of mitochondrial Cyt C were significantly inhibited , while Bcl-2 expression was increased .CONCLUSION: Tanshinone IIA prevents high glucose-induced endothelial cell apoptosis via mitochondria-dependent pathway .
