CAJ | 학술논문

本试验采用反转录聚合酶链式反应( RT-PCR)和cDNA末端快速扩增( RACE)技术克隆花鲈( Lateolabrax japonicus)调控摄食因子雷帕霉素靶蛋白( mTOR)、核糖体蛋白S6激酶be-ta-1( S6K1)、神经肽Y前体( NPY precursor)和脑肠肽前体( preproghrelin) cDNA全长序列. 结果显示:花鲈mTOR( GenBank登录号KJ746670) cDNA全长8 296 bp,开放阅读框7 551 bp,编码2 516个氨基酸,预测其分子质量为286.14 ku,与其他物种的相似性为62.0%~98.0%;S6K1 ( GenBank登录号KJ746671) cDNA全长2 232 bp,开放阅读框1 539 bp,编码512个氨基酸,预测其分子质量为56.87 ku,与其他物种的相似性为80.4%~84.9%;NPY precursor( GenBank登录号KJ850326) cDNA全长676 bp,开放阅读框300 bp,编码99 个氨基酸,预测其分子质量为11.26 ku,与其他物种的相似性为 53. 6%~99. 0%;preproghrelin ( GenBank 登录号 KJ850327 ) cDNA全长1 201 bp,开放阅读框324 bp,编码107个氨基酸,预测其分子质量为12.03 ku,与其他物种的相似性为19.6%~85.0%. 花鲈摄食调控因子cDNA全长序列地获得将为进一步研究其摄食调控机理奠定基础.
본시험채용반전록취합매련식반응( RT-PCR)화cDNA말단쾌속확증( RACE)기술극륭화로( Lateolabrax japonicus)조공섭식인자뢰파매소파단백( mTOR)、핵당체단백S6격매be-ta-1( S6K1)、신경태Y전체( NPY precursor)화뇌장태전체( preproghrelin) cDNA전장서렬. 결과현시:화로mTOR( GenBank등록호KJ746670) cDNA전장8 296 bp,개방열독광7 551 bp,편마2 516개안기산,예측기분자질량위286.14 ku,여기타물충적상사성위62.0%~98.0%;S6K1 ( GenBank등록호KJ746671) cDNA전장2 232 bp,개방열독광1 539 bp,편마512개안기산,예측기분자질량위56.87 ku,여기타물충적상사성위80.4%~84.9%;NPY precursor( GenBank등록호KJ850326) cDNA전장676 bp,개방열독광300 bp,편마99 개안기산,예측기분자질량위11.26 ku,여기타물충적상사성위 53. 6%~99. 0%;preproghrelin ( GenBank 등록호 KJ850327 ) cDNA전장1 201 bp,개방열독광324 bp,편마107개안기산,예측기분자질량위12.03 ku,여기타물충적상사성위19.6%~85.0%. 화로섭식조공인자cDNA전장서렬지획득장위진일보연구기섭식조공궤리전정기출.
The full-length cDNAs of food intake regulation factors, including mechanistic target of rapamycin ( mTOR) , ribosomal protein S6 kinase beta-1 ( S6K1) , neuropeptide Y ( NPY) precursor and preproghrelin were cloned from Japanese seabass ( Lateolabrax japonicus) using reverse transcription-polymerase chain reac-tion ( RT-PCR) and rapid amplification of cDNA end ( RACE) methods. The results showed as follows: the full-length mTOR cDNA of Japanese seabass ( GenBank accession No. KJ746670) was 8 296 bp, consisting of an open reading frame ( ORF) of 7 551 bp encoding a polypeptide of 2 516 amino acids with a theoretical molecular weight of 286.14 ku, and shared 62.0% to 98.0% similarity with other species. The full-length S6K1 cDNA of Japanese seabass ( GenBank accession No. KJ746671 ) was 2 232 bp, consisting of an ORF of 1 539 bp encoding a polypeptide of 512 amino acids with a theoretical molecular weight of 56. 87 ku, and shared 80.4% to 84.9% similarity with other species. The full-length NPY precursor cDNA of Japanese seabass ( GenBank accession No. KJ850326) was 676 bp, consisting of an ORF of 300 bp encoding a polypeptide of 99 amino acids with a theoretical molecular weight of 11.26 ku, and shared 53.6% to 99.0% similarity with other species. The full-length preproghrelin cDNA of Japanese seabass ( GenBank accession No. KJ850327) was 1 201 bp, consisting of an ORF of 324 bp encoding a polypeptide of 107 amino acids with a theoretical molecular weight of 12.03 ku, and shared 19. 6% to 85. 0% similarity with other species. The results of this study will provide a scientific basis for further study to explain regulatory mechanism of the factors of food in-take regulation in Japanese seabass.[Chinese Journal of Animal Nutrition, 2015, 27(9):2757-2773].