CAJ | 학술논문

目的：研究急性炎症状态下大鼠肝 P －糖蛋白翻译后修饰的机制。方法20只大鼠随机分成2组，模型组10只，对照组10只。模型组予以腹腔单次注射5 mg? kg－1脂多糖，对照组予以腹腔单次注射等体积0．9％氯化钠。24 h后，取肝组织，制备匀浆，分别用Western blot、免疫沉淀法和荧光法检测模型组和对照组的肝P－糖蛋白表达水平、P－糖蛋白泛素化水平和26S蛋白体活性的变化。结果与对照组相比，模型组肝 P －糖蛋白蛋白表达水平显著降低（P＜0．01），肝P－糖蛋白泛素化水平显著升高（P＜0．01），26S蛋白体的活性显著升高（P＜0．01）。结论泛素－蛋白酶体途径参与急性炎症状态下大鼠肝P－糖蛋白的下调。
목적：연구급성염증상태하대서간 P －당단백번역후수식적궤제。방법20지대서수궤분성2조，모형조10지，대조조10지。모형조여이복강단차주사5 mg? kg－1지다당，대조조여이복강단차주사등체적0．9％록화납。24 h후，취간조직，제비균장，분별용Western blot、면역침정법화형광법검측모형조화대조조적간P－당단백표체수평、P－당단백범소화수평화26S단백체활성적변화。결과여대조조상비，모형조간 P －당단백단백표체수평현저강저（P＜0．01），간P－당단백범소화수평현저승고（P＜0．01），26S단백체적활성현저승고（P＜0．01）。결론범소－단백매체도경삼여급성염증상태하대서간P－당단백적하조。
Objective To explore the expression of P -glycoprotein ( P-gp) in rat liver during acute inflammation ( AI ) and the possible mechanism involved.Methods Twenty rats were randomly divided into two groups:model group (n=10) and control group (n=10).The rats in model group were treated by a single 5 mg? kg -1 intraperitoneal injec-tion of lipopolysaccharide ( LPS).Control rats received equivalent injec-tion of sterile normal saline.The levels of P-gp, ubiquitinated P-gp and 26 S proteasome activity in both groups were analyzed by Western blot, immunoprecipitation and fluorescence detection, respectively. Results Compared to the control groups, ubiquitination levels of liver P-gp and 26S proteasome activity in model groups were significantly in-creased ( P<0.01 ) , accompanied by an decrease of liver P-gp protein expression level. Conclusion The participation of the ubiquitin -proteasome system in down-regulation of liver P-gp expression levels under AI conditions.