CAJ | 학술논문

目的：探讨儿童非典型呼吸道病原体感染与血清总Ig E水平的相关性。方法应用呼吸道九联检检测试剂盒（间接免疫荧光法）对1913例儿童呼吸道感染患者的血清样本进行9项非典型呼吸道病原体的Ig M抗体检测，包括肺炎支原体（M P ）、嗜肺军团菌（Lp）、Q热立克次体（QFR）、肺炎衣原体（CPn）、腺病毒（Adv）、呼吸道合胞病毒（RSV）、甲型流感病毒（IAv）、乙型流感病毒（IBv）和副流感病毒（PIV）1、2和3型；同时应用免疫散射比浊法检测这些血清样本的总IgE水平。对所得数据进行统计学分析。结果1913例儿童呼吸道感染患者中检出有非典型病原体感染者991例（阳性组），无非典型病原体感染者922例（阴性组）；阳性组以M P及M P合并其他病毒感染者为主，共650例（占65．59％）。阳性组患者血清总Ig E水平明显高于对照组，且MP感染患儿血清总IgE水平高于IBv、Adv及RSV感染者。在总IgE水平高于临床参考范围上限（100 kU／mL）的患者中，单纯MP感染者占31．29％，明显高于IgE低水平组（≤100 kU／mL）中的比例（21．30％）；而单纯RSV感染者占1．88％，明显低于IgE低水平组中的比例（6．53％）；单纯A dv感染者占3．13％，明显低于低水平组中的比例（6．53％）。结论在儿童非典型呼吸道病原体感染中以MP感染最常见；MP感染者较非MP感染者血清总IgE水平更高。
목적：탐토인동비전형호흡도병원체감염여혈청총Ig E수평적상관성。방법응용호흡도구련검검측시제합（간접면역형광법）대1913례인동호흡도감염환자적혈청양본진행9항비전형호흡도병원체적Ig M항체검측，포괄폐염지원체（M P ）、기폐군단균（Lp）、Q열립극차체（QFR）、폐염의원체（CPn）、선병독（Adv）、호흡도합포병독（RSV）、갑형류감병독（IAv）、을형류감병독（IBv）화부류감병독（PIV）1、2화3형；동시응용면역산사비탁법검측저사혈청양본적총IgE수평。대소득수거진행통계학분석。결과1913례인동호흡도감염환자중검출유비전형병원체감염자991례（양성조），무비전형병원체감염자922례（음성조）；양성조이M P급M P합병기타병독감염자위주，공650례（점65．59％）。양성조환자혈청총Ig E수평명현고우대조조，차MP감염환인혈청총IgE수평고우IBv、Adv급RSV감염자。재총IgE수평고우림상삼고범위상한（100 kU／mL）적환자중，단순MP감염자점31．29％，명현고우IgE저수평조（≤100 kU／mL）중적비례（21．30％）；이단순RSV감염자점1．88％，명현저우IgE저수평조중적비례（6．53％）；단순A dv감염자점3．13％，명현저우저수평조중적비례（6．53％）。결론재인동비전형호흡도병원체감염중이MP감염최상견；MP감염자교비MP감염자혈청총IgE수평경고。
Objective To investigate the correlation between atypical respiratory pathogens infection and serum total IgE levels . Methods Serum IgM level was detected in 1 913 blood samples of children with atypical respiratory infection by using indirect im‐munofluorescence assay ,including mycoplasma pneumonia(MP) ,legionella pneumophila (LP) ,rickettsia Q(QFR) ,chlamydia pneu‐monia(CPn) ,adenovirus (Adv) ,respiratory syncytial virus (RSV) ,influenza A virus (IAv) ,influenza B virus (IBv) and parainflu‐enza virus (PIV)1/2/3 .The serum total IgE level was detected by immune scatter turbidimetry .Software SPSS 17 .0 was used in data statistical analysis .Results A total of 991 out of 1 913 samples of respiratory inflected children exhibited positive(positive group) ,while 922 exhibited negative(negative group) in indirect immunofluorescence assay .650 out of the 991 positive samples (65 .59% ) contained MP infection and the combination of MP infection and other virus infections .The serum total IgE level in posi‐tive group was significantly higher than that of the negative group ,and the serum total IgE level in samples with MP infection was higher than that in samples with IBv infection ,Adv infection ,and RSV infection .In the samples in which serum total IgE level was higher than the clinical reference range (100 kU/mL) ,the infection rate of MP infection alone was 31 .29% ,which was evidently higher than that in samples of low IgE level(< 100 kU/mL ,21 .30% ) .On the other hand ,the infection rate of RSV alone was 1 .88% and the infection rate of Adv alone was 3 .13% ,which were both evidently lower than those in samples with normal serum total IgE level(both 6 .53% ) .Conclusion MP is the most common pathogen in children with atypical respiratory pathogen infec‐tion ,and can lead to higher serum total IgE levels .