CAJ | 학술논문

目的：研究云南白药对金黄色葡萄球菌溶血现象和血浆凝固能力的影响，以抗毒力为立足点探讨云南白药的新作用。方法取金黄色葡萄球菌标准菌株（ATCC29213）配制为0．5麦氏浊度单位（MCF）的菌液分别点种于含不同浓度云南白药的血琼脂平板中，37℃培养24 h后观察并测量溶血环直径。取不同浓度药液作用后的金黄色葡萄球菌配制成0．1MCF的菌液分别取20μL加于80μL的血浆中，37℃培养24h，观察凝固现象。结果4种浓度药液对金黄色葡萄球菌溶血环的影响有明显差异（P＜0．05），溶血环直径随药物浓度的增加而减小；对血浆的凝固作用影响不明显且没有规律性，差异无统计学意义（P＞0．05）。结论云南白药可以抑制金黄色葡萄球菌的生长和溶血能力，但对血浆凝固酶的作用不明显。
목적：연구운남백약대금황색포도구균용혈현상화혈장응고능력적영향，이항독력위립족점탐토운남백약적신작용。방법취금황색포도구균표준균주（ATCC29213）배제위0．5맥씨탁도단위（MCF）적균액분별점충우함불동농도운남백약적혈경지평판중，37℃배양24 h후관찰병측량용혈배직경。취불동농도약액작용후적금황색포도구균배제성0．1MCF적균액분별취20μL가우80μL적혈장중，37℃배양24h，관찰응고현상。결과4충농도약액대금황색포도구균용혈배적영향유명현차이（P＜0．05），용혈배직경수약물농도적증가이감소；대혈장적응고작용영향불명현차몰유규률성，차이무통계학의의（P＞0．05）。결론운남백약가이억제금황색포도구균적생장화용혈능력，단대혈장응고매적작용불명현。
Objective To Study the effect of Yunnan Baiyao on the hemolysis and plasma coagulation ability of Staphylococcus aureus.ToseeknewmechanismofYunnanBaiyao′sapplicationonthebasisofanti‐virulence.Methods 0.5MCFbacterialiquidof Staphylococcus aureus standard strains(ATCC29213) were prepared and cultured in blood agar plates which containing Yunnan Baiyao of different concentrations at 37 ℃ for 24 h .Then the diameter of hemolysis rings were measured and recorded .0 .1 MCF bacteria liquids treated with Yunan Baiyao of different concentrations were prepared ,and 20μL of the bacteria liquid were added in‐to 80 μL plasma ,then cultivated at 37 ℃ for 24 h to observe the phenomenon of solidification .Results There was significant differ‐ence among the effects of Yunan Baiyao of different concentrations on the Staphylococcus aureus hemolytic rings(P<0 .05) ,and the drug concentrations inversely proportional to the diameter of hemolysis .Plasma coagulation effect of bacteria was not obvious differ‐ent after the treatment with different concentrations of Yunnan Baiyao ,and not had any regular pattern(P>0 .05) .Conclusion Yunnan baiyao can inhibit the growth of Staphylococcus aureus and its hemolytic ability but has no significant effect on plasma clot‐ting enzyme .