CAJ | 학술논문

本文旨在研究低饲粮阴阳离子差( DCAD)条件下添加维生素D对黔北麻羊妊娠母羊血浆钙及其调控因子浓度的影响. 将18头健康妊娠母羊随机分为3组,每组6个重复,每个重复1只羊,各组分别饲喂DCAD为212.4(对照)、-145.5(Ⅰ)、-145.5 mmol/kg(Ⅱ) (干物质基础,实测值)的饲粮. 其中,对照组饲喂基础饲粮,Ⅰ组在对照组基础上添加45 g/d六水合氯化镁,Ⅱ组在Ⅰ组基础上添加2 000 IU/d维生素D. 预试期10 d,正试期30 d. 结果表明:1)Ⅰ、Ⅱ组尿液pH显著低于对照组(P<0.05). 3组山羊生长性能不受饲粮处理的影响(P>0.05).2)与对照组相比,Ⅰ( P>0.05)、Ⅱ组( P<0.05)血浆钙浓度均升高;对照组、Ⅰ、Ⅱ组血浆磷、甲状旁腺激素、降钙素浓度没有显著差异( P>0.05) ,Ⅱ组较Ⅰ组和对照组显著提高了血浆1,25二羟基维生素D3 浓度( P<0.05). 3)Ⅱ组血浆维生素D受体、钙结合蛋白D9k浓度显著高于对照组( P<0.05). 在本试验条件下,在低DCAD条件下添加维生素D能更有助于维持动物血液钙恒稳状态.
본문지재연구저사량음양리자차( DCAD)조건하첨가유생소D대검북마양임신모양혈장개급기조공인자농도적영향. 장18두건강임신모양수궤분위3조,매조6개중복,매개중복1지양,각조분별사위DCAD위212.4(대조)、-145.5(Ⅰ)、-145.5 mmol/kg(Ⅱ) (간물질기출,실측치)적사량. 기중,대조조사위기출사량,Ⅰ조재대조조기출상첨가45 g/d륙수합록화미,Ⅱ조재Ⅰ조기출상첨가2 000 IU/d유생소D. 예시기10 d,정시기30 d. 결과표명:1)Ⅰ、Ⅱ조뇨액pH현저저우대조조(P<0.05). 3조산양생장성능불수사량처리적영향(P>0.05).2)여대조조상비,Ⅰ( P>0.05)、Ⅱ조( P<0.05)혈장개농도균승고;대조조、Ⅰ、Ⅱ조혈장린、갑상방선격소、강개소농도몰유현저차이( P>0.05) ,Ⅱ조교Ⅰ조화대조조현저제고료혈장1,25이간기유생소D3 농도( P<0.05). 3)Ⅱ조혈장유생소D수체、개결합단백D9k농도현저고우대조조( P<0.05). 재본시험조건하,재저DCAD조건하첨가유생소D능경유조우유지동물혈액개항은상태.
This study was conducted to evaluate the synergy effects of low dietary cation-anion difference (DCAD) with vitamin D on plasma concentrations of calcium (Ca) and its modulators of pregnant goats. Eighteen healthy pregnant goats were randomly allocated to 3 blocks with 6 replicates per group and 1 goat per replicate. Goats were fed 3 DCAD at 212.4 (control), -145.5 (Ⅰ), -145.5 mmol/kg (Ⅱ) [dry matter (DM) basis, measured values], respectively. Control group was fed a basal diet, 45 g/d MgCl2·6H2O was added in groupⅠon the basis of the basal diet, and 2 000 IU/d vitamin D was added in groupⅡon the basis of group Ⅰ. The pre-test lasted for 10 d, and the test lasted for 30 d. The results showed as follow:1) urine pH was significantly reduced in groups Ⅰ and Ⅱ over control ( P<0.05) . For the growth performance meas-urements DM intake, average daily gain, and feed to gain ratio were not affected by dietary treatments ( P>0.05) . 2) Compared with control group, plasma Ca concentration was increased in groups Ⅰ ( P>0.05) andⅡ ( P<0.05); there were no significant differences in plasma phosphorus, parathormone and calcitonin con-centrations for control group and groups Ⅰ and Ⅱ (P>0.05), but group Ⅱ significantly increased plasma 1,25-(OH)2VD3 concentration compared with control group and group Ⅰ (P<0.05). 3) Plasma vitamin D receptor and Ca-binding protein-D9k concentrations in group Ⅱ were significantly increased compared with control group ( P<0.05) . These results suggest that blood Ca homeostasis can be more available to maintain by low DCAD synergized with vitamin D under the conditions in the present study.