中国全科医学
中國全科醫學
중국전과의학
Chinese General Practice
2015年
27期
3355-3359
,共5页
许宁%平芬%徐鑫%诸葛铭宁%张凤蕊%韩书芝
許寧%平芬%徐鑫%諸葛銘寧%張鳳蕊%韓書芝
허저%평분%서흠%제갈명저%장봉예%한서지
肺炎%颗粒物%支气管肺泡灌洗液%白细胞介素%肿瘤坏死因子α
肺炎%顆粒物%支氣管肺泡灌洗液%白細胞介素%腫瘤壞死因子α
폐염%과립물%지기관폐포관세액%백세포개소%종류배사인자α
Pneumonia%Particulate matter%Bronchoalveolar lavage fluid%Interleukin%Tumor necrosis factor-alpha
目的：探讨细颗粒物（ PM2.5）急性暴露对大鼠肺部炎性损伤的作用，及低、中、高剂量连花清瘟对肺部炎性损伤的拮抗作用。方法2013年6—12月选取48只健康成年Wistar大鼠，采用随机数字表法分为空白对照组、0.9%氯化钠溶液对照组、PM2.5染尘组及低、中、高剂量连花清瘟组，每组8只。由石家庄市环境监测中心提供PM2.5，制备PM2.5悬液。空白对照组大鼠无任何干预措施；0.9%氯化钠溶液对照组大鼠给予气管滴注0.9%氯化钠溶液（1 ml/kg）；PM2.5染尘组大鼠给予气管滴注PM2.5悬液1 ml/kg（7.5 mg/kg）；低、中、高剂量连花清瘟组大鼠首先分别连续灌胃给予低（2 g/kg）、中（4 g/kg）、高（8 g/kg）剂量连花清瘟溶液10 ml/kg，共4 d，第4天灌胃给药后分别气管滴注 PM2.5悬液1 ml/kg（7.5 mg/kg），24 h 后处死。光镜下观察肺组织病理形态变化，酶联免疫吸附试验（ELISA）法测定各组大鼠支气管肺泡灌洗液（BALF）及血清中白介素（IL）1、IL-6、肿瘤坏死因子α（TNF-α）水平。结果空白对照组及0.9%氯化钠溶液对照组大鼠肺组织病理切片光镜下未见异常表现；PM2.5染尘组大鼠肺组织病理切片光镜下可见炎性细胞渗出，肺间质纤维组织增生，间质水肿；低、中、高剂量连花清瘟组大鼠肺组织病理切片光镜下可见随连花清瘟剂量增加，肺泡腔内炎性细胞渗出逐渐减轻。0.9%氯化钠溶液对照组大鼠BALF及血清中IL-1、IL-6、TNF-α水平与空白对照组比较，差异均无统计学意义（ P>0.05）；PM2.5染尘组大鼠BALF中IL-1水平较0.9%氯化钠溶液对照组升高，血清中IL-1水平、BALF及血清中IL-6、TNF-α水平较空白对照组和0.9%氯化钠溶液对照组升高（ P<0.05）；高剂量连花清瘟组大鼠BALF中IL-1水平较空白对照组、0.9%氯化钠溶液对照组、PM2.5染尘组、低剂量连花清瘟组、中剂量连花清瘟组降低，BALF和血清中IL-6水平较空白对照组、0.9%氯化钠溶液对照组、PM2.5染尘组、低剂量连花清瘟组降低，BALF中TNF-α水平和血清中IL-1水平较PM2.5染尘组、低剂量连花清瘟组、中剂量连花清瘟组降低，血清中TNF-α水平较PM2.5染尘组、低剂量连花清瘟组降低（P<0.05）。结论PM2.5急性暴露可以导致大鼠肺部炎性损伤，连花清瘟对肺部炎性损伤有拮抗作用。
目的：探討細顆粒物（ PM2.5）急性暴露對大鼠肺部炎性損傷的作用，及低、中、高劑量連花清瘟對肺部炎性損傷的拮抗作用。方法2013年6—12月選取48隻健康成年Wistar大鼠，採用隨機數字錶法分為空白對照組、0.9%氯化鈉溶液對照組、PM2.5染塵組及低、中、高劑量連花清瘟組，每組8隻。由石傢莊市環境鑑測中心提供PM2.5，製備PM2.5懸液。空白對照組大鼠無任何榦預措施；0.9%氯化鈉溶液對照組大鼠給予氣管滴註0.9%氯化鈉溶液（1 ml/kg）；PM2.5染塵組大鼠給予氣管滴註PM2.5懸液1 ml/kg（7.5 mg/kg）；低、中、高劑量連花清瘟組大鼠首先分彆連續灌胃給予低（2 g/kg）、中（4 g/kg）、高（8 g/kg）劑量連花清瘟溶液10 ml/kg，共4 d，第4天灌胃給藥後分彆氣管滴註 PM2.5懸液1 ml/kg（7.5 mg/kg），24 h 後處死。光鏡下觀察肺組織病理形態變化，酶聯免疫吸附試驗（ELISA）法測定各組大鼠支氣管肺泡灌洗液（BALF）及血清中白介素（IL）1、IL-6、腫瘤壞死因子α（TNF-α）水平。結果空白對照組及0.9%氯化鈉溶液對照組大鼠肺組織病理切片光鏡下未見異常錶現；PM2.5染塵組大鼠肺組織病理切片光鏡下可見炎性細胞滲齣，肺間質纖維組織增生，間質水腫；低、中、高劑量連花清瘟組大鼠肺組織病理切片光鏡下可見隨連花清瘟劑量增加，肺泡腔內炎性細胞滲齣逐漸減輕。0.9%氯化鈉溶液對照組大鼠BALF及血清中IL-1、IL-6、TNF-α水平與空白對照組比較，差異均無統計學意義（ P>0.05）；PM2.5染塵組大鼠BALF中IL-1水平較0.9%氯化鈉溶液對照組升高，血清中IL-1水平、BALF及血清中IL-6、TNF-α水平較空白對照組和0.9%氯化鈉溶液對照組升高（ P<0.05）；高劑量連花清瘟組大鼠BALF中IL-1水平較空白對照組、0.9%氯化鈉溶液對照組、PM2.5染塵組、低劑量連花清瘟組、中劑量連花清瘟組降低，BALF和血清中IL-6水平較空白對照組、0.9%氯化鈉溶液對照組、PM2.5染塵組、低劑量連花清瘟組降低，BALF中TNF-α水平和血清中IL-1水平較PM2.5染塵組、低劑量連花清瘟組、中劑量連花清瘟組降低，血清中TNF-α水平較PM2.5染塵組、低劑量連花清瘟組降低（P<0.05）。結論PM2.5急性暴露可以導緻大鼠肺部炎性損傷，連花清瘟對肺部炎性損傷有拮抗作用。
목적：탐토세과립물（ PM2.5）급성폭로대대서폐부염성손상적작용，급저、중、고제량련화청온대폐부염성손상적길항작용。방법2013년6—12월선취48지건강성년Wistar대서，채용수궤수자표법분위공백대조조、0.9%록화납용액대조조、PM2.5염진조급저、중、고제량련화청온조，매조8지。유석가장시배경감측중심제공PM2.5，제비PM2.5현액。공백대조조대서무임하간예조시；0.9%록화납용액대조조대서급여기관적주0.9%록화납용액（1 ml/kg）；PM2.5염진조대서급여기관적주PM2.5현액1 ml/kg（7.5 mg/kg）；저、중、고제량련화청온조대서수선분별련속관위급여저（2 g/kg）、중（4 g/kg）、고（8 g/kg）제량련화청온용액10 ml/kg，공4 d，제4천관위급약후분별기관적주 PM2.5현액1 ml/kg（7.5 mg/kg），24 h 후처사。광경하관찰폐조직병리형태변화，매련면역흡부시험（ELISA）법측정각조대서지기관폐포관세액（BALF）급혈청중백개소（IL）1、IL-6、종류배사인자α（TNF-α）수평。결과공백대조조급0.9%록화납용액대조조대서폐조직병리절편광경하미견이상표현；PM2.5염진조대서폐조직병리절편광경하가견염성세포삼출，폐간질섬유조직증생，간질수종；저、중、고제량련화청온조대서폐조직병리절편광경하가견수련화청온제량증가，폐포강내염성세포삼출축점감경。0.9%록화납용액대조조대서BALF급혈청중IL-1、IL-6、TNF-α수평여공백대조조비교，차이균무통계학의의（ P>0.05）；PM2.5염진조대서BALF중IL-1수평교0.9%록화납용액대조조승고，혈청중IL-1수평、BALF급혈청중IL-6、TNF-α수평교공백대조조화0.9%록화납용액대조조승고（ P<0.05）；고제량련화청온조대서BALF중IL-1수평교공백대조조、0.9%록화납용액대조조、PM2.5염진조、저제량련화청온조、중제량련화청온조강저，BALF화혈청중IL-6수평교공백대조조、0.9%록화납용액대조조、PM2.5염진조、저제량련화청온조강저，BALF중TNF-α수평화혈청중IL-1수평교PM2.5염진조、저제량련화청온조、중제량련화청온조강저，혈청중TNF-α수평교PM2.5염진조、저제량련화청온조강저（P<0.05）。결론PM2.5급성폭로가이도치대서폐부염성손상，련화청온대폐부염성손상유길항작용。
Objective To investigate the pulmonary inflammatory injury in rats induced by acute exposure to PM2. 5 and to explore the antagonistic effect of low,medium,and high dose of Lianhuaqingwen to the inflammatory injury. Methods From June to December in 2013,selected 48 healthy adult Wistar rats. Using random number table method,divided them into control group,sodium chloride group,PM2. 5 group,low-dose group,medium-dose group and high-dose group,with 8 rats in each group. Airborne fine particulate matters were provided by Shijiazhuang Environmental Monitoring Center,and PM2. 5 suspension liquid was prepared. Control group was not given any intervention;sodium chloride group was given trachea instillation of 0. 9%sodium chloride solution( 1 ml/kg );PM2. 5 group was given trachea instillation of 1 ml/kg ( 7. 5 mg/kg ) PM2. 5 suspension liquid;low-dose,medium-dose and high -dose groups were respectively given gavage with 2 g/kg,4 g/kg and 8 g/kg Lianhuaqingwen solution of 10 ml/kg for 4 days,and the three groups were given trachea instillation of 1 ml/kg(7. 5 mg/kg) PM2. 5 suspension liquid on the fourth day and were killed 24 hours later. Pathological morphologic changes were observed under light microscope,and ELISA was employed to examine the levels of IL-1,IL-6 and TNF-αin BALF and serum. Results In control group and sodium chloride group, no abnormal manifestations in pulmonary tissue slices were observed under light microscope;in PM2. 5 group,exudation of inflammatory cells,interstitial proliferation of fibrous tissue and interstitial edema were noted in pulmonary tissue slices under light microscope;in the three Lianhuaqingwen groups, we found the exudation of inflammatory cells in alveolar space reduced with the increase of the dosage of Lianhuaqingwen. Sodium chloride group and control group were not significantly different in the levels of IL-1,IL-6 and TNF-α in BALF and serum(P>0. 05);PM2.5 group was higher than sodium chloride group in the level of IL-1 in BALF,and PM2. 5 group was higher than control group and sodium chloride group in the level of IL-1 in serum and the levels of IL-6 and TNF-αin BALF and serum(P<0. 05);high-dose group was lower than control group,sodium chloride group,PM2. 5 group,low-dose group and medium group in the level of IL-1 in BALF,lower than control group,sodium chloride group,PM2. 5 group and low-dose group in the level of IL-6 in BALF and serum,lower than PM2. 5 group,low-dose group and medium-dose group in the level of TNF-α in BALF and the level of IL-1 in serum, and lower than PM2. 5 group and low -dose group in the level of TNF -α in serum ( P <0. 05 ). Conclusion The acute exposure to PM2. 5 can induce pulmonary inflammatory injury in rats, Lianhuaqingwen could produce antagonistic effects to the inflammatory injury.
