CAJ | 학술논문

目的：精原相关蛋白9（ SPAG9）对人骨肉瘤细胞株U2OS迁移、侵袭能力的影响。方法 siRNA干扰U2OS细胞SPAG9的表达，对照组转染U2OS细胞对照 siRNA，实验组转染 U2OS 细胞 SPAG9的 siRNA，用细胞计数试剂盒（CCK－8）检测SPAG9对细胞增殖能力的影响，用划痕实验、Transwell小室模型实验测定细胞迁移、侵袭能力改变情况，以免疫印迹实验测定细胞基质金属蛋白酶－2（MMP－2）、基质金属蛋白酶－9（MMP－9）的蛋白变化，以明胶酶谱实验测定细胞分泌MMP－2、MMP－9的变化。结果特异性 SPAG9基因 siRNA 可明显抑制 U2OS 细胞中SPAG9的表达；沉默 SPAG9后，U2OS细胞的增殖能力无明显变化，而划痕愈合能力、迁移活力及侵袭能力较对照组细胞明显下降，其分泌MMP－2、MMP－9的水平也明显下降。结论沉默SPAG9可致人骨肉瘤细胞U2OS的迁移和侵袭能力下降。
목적：정원상관단백9（ SPAG9）대인골육류세포주U2OS천이、침습능력적영향。방법 siRNA간우U2OS세포SPAG9적표체，대조조전염U2OS세포대조 siRNA，실험조전염 U2OS 세포 SPAG9적 siRNA，용세포계수시제합（CCK－8）검측SPAG9대세포증식능력적영향，용화흔실험、Transwell소실모형실험측정세포천이、침습능력개변정황，이면역인적실험측정세포기질금속단백매－2（MMP－2）、기질금속단백매－9（MMP－9）적단백변화，이명효매보실험측정세포분비MMP－2、MMP－9적변화。결과특이성 SPAG9기인 siRNA 가명현억제 U2OS 세포중SPAG9적표체；침묵 SPAG9후，U2OS세포적증식능력무명현변화，이화흔유합능력、천이활력급침습능력교대조조세포명현하강，기분비MMP－2、MMP－9적수평야명현하강。결론침묵SPAG9가치인골육류세포U2OS적천이화침습능력하강。
Objective To investigate the effect of sperm -associated antigen 9 ( SPAG9 ) on migration and invasion abilities of human osteo-sarcoma U2OS cells.Methods siRNA was used to decrease SPAG9 expression, and then cells were subjected to CCK -8 assay, wound healing assay and transwell assay to detect siSPAG9 on cell migration and invasion abilities. Western blot and Gelatin zymography were used to detect matrix metalloproteinase-2 ( MMP-2) and matrix metalloprotei-nase-9 ( MMP -9 ) expression and activities. Results siRNA for SPAG9 significantly decreased SPAG9 mRNA and protein expression. siSPAG9 had no effect on the proliferation of U2OS cells, but significant-ly inhibited its wound healing, migration and invasion abilities. The activities and expression of MMP-2 amd MMP-9 were also suppressed by siRNA for SPAG9.Conclusion Depletion of SPAG9 results in the inhi-bition of migration and invasion abilities of human osteosarcoma U2OS cells.