激光生物学报
激光生物學報
격광생물학보
Acta Laser Biology Sinica
2015年
4期
390-399
,共10页
宋欣%孙敏%高志强%任爱霞%赵红梅%李光%郝兴宇%杨珍平%宗毓铮
宋訢%孫敏%高誌彊%任愛霞%趙紅梅%李光%郝興宇%楊珍平%宗毓錚
송흔%손민%고지강%임애하%조홍매%리광%학흥우%양진평%종육쟁
旱地小麦%休闲期覆盖%磷肥%氮代谢关键酶%籽粒蛋白质
旱地小麥%休閒期覆蓋%燐肥%氮代謝關鍵酶%籽粒蛋白質
한지소맥%휴한기복개%린비%담대사관건매%자립단백질
dryland wheat%mulch in fallow period%phosphorus%key enzymes of nitrogen metabolism%grain protein
为探索旱地小麦休闲期覆盖保水配施磷肥高产、优质的技术途径,在山西农业大学闻喜试验基地采用大田试验研究了休闲期覆盖或不覆盖条件下低(75 kg/hm2)、中(112.5 kg/hm2)、高(150 kg/hm2)施磷水平对土壤水分、籽粒蛋白质形成的影响及其生理机制。结果表明:休闲期覆盖后,播种期0-100 cm土壤蓄水量显著提高,达39-42 mm,而开花期60-100 cm土层降低。覆盖后,花后旗叶和籽粒GS和GOGAT活性提高,籽粒游离氨基酸和灌浆后期GMP含量提高,籽粒蛋白质及其组分含量、谷/醇均提高。增加施磷量,开花期20-60 cm、80-100 cm土层蓄水量降低,而花后旗叶和籽粒GS活性提高,且覆盖条件下花后0-15 d、20 d旗叶GOGAT活性,花后5 d、15 d、25-30 d籽粒GOGAT活性,籽粒游离氨基酸含量、籽粒GMP含量提高,籽粒蛋白质及其组分含量、谷/醇、蛋白质产量显著提高,产量提高940-1452 kg/hm2。此外,休闲期覆盖配施磷肥条件下,开花期深层土壤水分与旗叶GS和GOGAT活性密切相关,旗叶和籽粒GS和GOGAT活性均与游离氨基酸含量、谷/醇、蛋白质产量关系密切。总之,旱地小麦休闲期覆盖有利于提高底墒,且配合施磷量150 kg/hm2可促进根系吸收深层土壤水分,提高产量的同时也提高了籽粒蛋白质含量、GMP含量和谷/醇。
為探索旱地小麥休閒期覆蓋保水配施燐肥高產、優質的技術途徑,在山西農業大學聞喜試驗基地採用大田試驗研究瞭休閒期覆蓋或不覆蓋條件下低(75 kg/hm2)、中(112.5 kg/hm2)、高(150 kg/hm2)施燐水平對土壤水分、籽粒蛋白質形成的影響及其生理機製。結果錶明:休閒期覆蓋後,播種期0-100 cm土壤蓄水量顯著提高,達39-42 mm,而開花期60-100 cm土層降低。覆蓋後,花後旂葉和籽粒GS和GOGAT活性提高,籽粒遊離氨基痠和灌漿後期GMP含量提高,籽粒蛋白質及其組分含量、穀/醇均提高。增加施燐量,開花期20-60 cm、80-100 cm土層蓄水量降低,而花後旂葉和籽粒GS活性提高,且覆蓋條件下花後0-15 d、20 d旂葉GOGAT活性,花後5 d、15 d、25-30 d籽粒GOGAT活性,籽粒遊離氨基痠含量、籽粒GMP含量提高,籽粒蛋白質及其組分含量、穀/醇、蛋白質產量顯著提高,產量提高940-1452 kg/hm2。此外,休閒期覆蓋配施燐肥條件下,開花期深層土壤水分與旂葉GS和GOGAT活性密切相關,旂葉和籽粒GS和GOGAT活性均與遊離氨基痠含量、穀/醇、蛋白質產量關繫密切。總之,旱地小麥休閒期覆蓋有利于提高底墑,且配閤施燐量150 kg/hm2可促進根繫吸收深層土壤水分,提高產量的同時也提高瞭籽粒蛋白質含量、GMP含量和穀/醇。
위탐색한지소맥휴한기복개보수배시린비고산、우질적기술도경,재산서농업대학문희시험기지채용대전시험연구료휴한기복개혹불복개조건하저(75 kg/hm2)、중(112.5 kg/hm2)、고(150 kg/hm2)시린수평대토양수분、자립단백질형성적영향급기생리궤제。결과표명:휴한기복개후,파충기0-100 cm토양축수량현저제고,체39-42 mm,이개화기60-100 cm토층강저。복개후,화후기협화자립GS화GOGAT활성제고,자립유리안기산화관장후기GMP함량제고,자립단백질급기조분함량、곡/순균제고。증가시린량,개화기20-60 cm、80-100 cm토층축수량강저,이화후기협화자립GS활성제고,차복개조건하화후0-15 d、20 d기협GOGAT활성,화후5 d、15 d、25-30 d자립GOGAT활성,자립유리안기산함량、자립GMP함량제고,자립단백질급기조분함량、곡/순、단백질산량현저제고,산량제고940-1452 kg/hm2。차외,휴한기복개배시린비조건하,개화기심층토양수분여기협GS화GOGAT활성밀절상관,기협화자립GS화GOGAT활성균여유리안기산함량、곡/순、단백질산량관계밀절。총지,한지소맥휴한기복개유리우제고저상,차배합시린량150 kg/hm2가촉진근계흡수심층토양수분,제고산량적동시야제고료자립단백질함량、GMP함량화곡/순。
In order to study the technical approach of mulching in fallow period and the application of phosphorus fertil-izer to improve yield and quality of dryland wheat, field trial was carried out to study the effect of two mulching situations and three fertilizer treatments on soil water and grain protein accumulation and its physiological mechanism in Wenxi ex-periment site of Shanxi Agricultural University. The three treatments of phosphorus fertilizer were: low-level ( 75 kg/hm2), middle-level (112. 5 kg/hm2) and high-level (150 kg/hm2). Two mulching situations were set up for each fer-tilizer treatment: mulch and no mulch in fallow period. The results showed that soil moisture storage at the depth of 0-100 cm increased significantly by 38-41 mm at sowing stage and at the depth of 60-100 cm layer decreased at anthesis under the situation of mulching in fallow period. After mulching in fallow period, the activity of GS and GOGAT in post-anthesis flag leaves and grains were improved. The contents of free amino acid in grain and GMP at late filling stage were enhanced. The contents of grain protein and its components and the ratio of Glu/Gli were both increased. Increasing the amount of phosphorus fertilizer, soil water storage in the 20-60 cm and 80-100 cm layer were reduced, however the ac-tivity of GS in post-anthesis flag leaves and grains were improved. In additon, both the activity of GOGAT in flag leaves of post-anthesis 5-15 d, 20 d, it in grain of post-anthesis 5 d, 15 d, 25-30 d and the contents of free amino acid and GMP in grain were enhanced. The contents of grain protein and its components, the ratio of Glu/Gli and protein yield were all improved significantly. The yield of wheat was increased by 940-1452 kg/hm2 . Furthermore, under the condi-tion of mulching and phosphorus fertilizer, there was a close relationship between deep soil moisture at anthesis and the activity of GS and GOGAT in flag leaves. All the activity of GS and GOGAT in flag leaves and grain were closely related to the contents of free amino acid, the ratio of Glu/Gli and protein yield. The above results suggested that mulching in fallow period in dryland wheat should be beneficial to improve soil moisture before sowing. And applying phosphorus fer-tilizer of 150 kg/hm2 can help roots absorb the soil water in deep layer. This measure can promote the increase of yield and also further improve the contents of grain protein and GMP and the ratio of Glu/Gli.