CAJ | 학술논문

目的：研究基质金属蛋白酶‐9（MMP‐9）蛋白及mRNA在乳腺癌中的表达，探讨其在乳腺癌发生、发展及转移过程中的临床意义。方法用免疫组化法检测M M P‐9蛋白在乳腺癌、乳腺良性病变组织中的表达，用半定量反转录聚合酶链反应（R T‐PCR）的方法检测组织中mRNA的表达。结果 MMP‐9蛋白在乳腺癌组织中的阳性表达率为69．6％（39／56），在乳腺良性病变组织中为20％（6／30），差异有统计学意义（P＜0．05）。MMP‐9 mRNA在乳腺癌和乳腺良性病变组织中表达水平（灰度比值）分别为0．9142±0．1081、0．3794±0．0428，两者比较差异有统计学意义（P＜0．05）。结论 MMP‐9蛋白及mRNA在乳腺癌组织中的表达与淋巴结转移呈正相关，有可能作为判断乳腺癌浸润转移能力的参考指标之一。
목적：연구기질금속단백매‐9（MMP‐9）단백급mRNA재유선암중적표체，탐토기재유선암발생、발전급전이과정중적림상의의。방법용면역조화법검측M M P‐9단백재유선암、유선량성병변조직중적표체，용반정량반전록취합매련반응（R T‐PCR）적방법검측조직중mRNA적표체。결과 MMP‐9단백재유선암조직중적양성표체솔위69．6％（39／56），재유선량성병변조직중위20％（6／30），차이유통계학의의（P＜0．05）。MMP‐9 mRNA재유선암화유선량성병변조직중표체수평（회도비치）분별위0．9142±0．1081、0．3794±0．0428，량자비교차이유통계학의의（P＜0．05）。결론 MMP‐9단백급mRNA재유선암조직중적표체여림파결전이정정상관，유가능작위판단유선암침윤전이능력적삼고지표지일。
Objective To study expression of matrix metalloproteinase‐9(MMP‐9) protein and its mRNA in breast cancer and e‐valuate its significance in the occurrence ,development and metastasis of breast cancer .Methods The protein expression of MMP‐9 breast cancer were detected by using immunohistochemistry and the expression of mRNA were detected by reverse transcription polymerase chain reaction(RT‐PCR)Results The positive rate of MMP‐9 protein expression in 56 cases of breast cancer was 69 .6% (39/56) ,while in benign breast diseases was 20% (6/30) ,which were significantly different(P<0 .05) .The levels of MMP‐9 mRNA were significantly higher in the patients with breast cancer than those in benign breast diseases(P<0 .05) ,which were 0 .914 2 ± 0 .108 1 and 0 .379 4 ± 0 .0428 respectively .Conclusion The MMP‐9 protein and mRNA expression in human breast cancers are positively correlated with the stage and lymph node metastasis .Expression of MMP‐9 could be used as an indicator for the ability of invasion and metastasis of breast cancer .