CAJ | 학술논문

目的鉴定小鼠造血体系发育过程中特异性表达的miRNAs,探讨其调控细胞终末分化的分子机制.方法 PMA诱导HL-60分化为巨噬细胞样细胞,Cayman吞噬作用检测试剂盒结合荧光显微镜显影检测诱导后细胞的吞噬作用,Real-time PCR检测巨噬细胞分化指标CD 11 b、CD 4以及miR-155的表达.结果成功建立使用PMA诱导人前髓性白血病细胞HL-60分化为巨噬细胞样细胞模型,并发现组织特异性miR-155随着巨噬细胞分化表达水平逐渐上调.结论造血体系特异miR-155参与调控巨噬细胞终末分化,揭示组织特异性miRNAs调控小鼠组织细胞分化和组织发育的普遍意义.
목적 감정소서조혈체계발육과정중특이성표체적miRNAs,탐토기조공세포종말분화적분자궤제.방법 PMA유도HL-60분화위거서세포양세포,Cayman탄서작용검측시제합결합형광현미경현영검측유도후세포적탄서작용,Real-time PCR검측거서세포분화지표CD 11 b、CD 4이급miR-155적표체.결과 성공건립사용PMA유도인전수성백혈병세포HL-60분화위거서세포양세포모형,병발현조직특이성miR-155수착거서세포분화표체수평축점상조.결론 조혈체계특이miR-155삼여조공거서세포종말분화,게시조직특이성miRNAs조공소서조직세포분화화조직발육적보편의의.
Objective To identify the specific miRNA during the development of hematopoietic system, and explore its molecular mechanism.Methods Induction of HL-60 differentiating into macrophage cells with PMA, detection of the phagocytosis with cayman phagocytosis assay kit, expression of miR-155, CD 11 b and CD 14 mRNA were tested by real-time PCR.Results Establish in the induction macrophage differentiation model of HL-60 by PMA successfully, and found that expression of miR-155 increased gradually during differentia.Conclusion Hematopoietic system specific miR-155 regulates macrophage terminal differentiation. This observation indicates an universal regulatory mechanism that different tissue-speciifc miRNAs are employed to control different tissues development.