中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
Chinese Journal of Pathophysiology
2015年
9期
1662-1666
,共5页
施益芬%甘一峰%沈志坚%俞康
施益芬%甘一峰%瀋誌堅%俞康
시익분%감일봉%침지견%유강
苯%SP1%组蛋白乙酰化%组蛋白甲基化%染色质免疫沉淀
苯%SP1%組蛋白乙酰化%組蛋白甲基化%染色質免疫沉澱
분%SP1%조단백을선화%조단백갑기화%염색질면역침정
Benzene%Sp1%Histone acetylation%Histone methylation%Chromatin immunoprecipitation
目的：研究拓扑异构酶Ⅱα（ TOPOⅡα）启动子调控因子SP1组蛋白化学修饰在苯中毒病人中的改变。方法：25例临床慢性苯中毒患者骨髓单个核细胞为病例组，25例正常人骨髓单个核细胞为对照组，染色质免疫沉淀技术探讨TOPOⅡα启动子调控因子Sp1组蛋白乙酰化和甲基化水平的变化，RT－PCR法测定Sp1 mRNA的表达水平。结果：与对照组相比，临床苯中毒病例TOPOⅡα启动子调控因子Sp1组蛋白H4和H3乙酰化水平下降（P＜0．01），组蛋白H3K9甲基化水平升高（P＜0．01），组蛋白H3K4甲基化水平无明显改变。与正常对照组相比，临床苯中毒病例TOPOⅡα启动子调控因子Sp1的mRNA表达水平降低（ P＜0．05）。结论：慢性苯中毒TO－POⅡα启动子调控因子Sp1组蛋白H4、H3乙酰化及H3K9甲基化修饰水平的改变伴随着mRNA水平的变化。TOPOⅡα启动子调控因子Sp1可能通过组蛋白H4、H3乙酰化及H3K9甲基化修饰改变在苯中毒所致的造血毒性中发挥作用。
目的：研究拓撲異構酶Ⅱα（ TOPOⅡα）啟動子調控因子SP1組蛋白化學脩飾在苯中毒病人中的改變。方法：25例臨床慢性苯中毒患者骨髓單箇覈細胞為病例組，25例正常人骨髓單箇覈細胞為對照組，染色質免疫沉澱技術探討TOPOⅡα啟動子調控因子Sp1組蛋白乙酰化和甲基化水平的變化，RT－PCR法測定Sp1 mRNA的錶達水平。結果：與對照組相比，臨床苯中毒病例TOPOⅡα啟動子調控因子Sp1組蛋白H4和H3乙酰化水平下降（P＜0．01），組蛋白H3K9甲基化水平升高（P＜0．01），組蛋白H3K4甲基化水平無明顯改變。與正常對照組相比，臨床苯中毒病例TOPOⅡα啟動子調控因子Sp1的mRNA錶達水平降低（ P＜0．05）。結論：慢性苯中毒TO－POⅡα啟動子調控因子Sp1組蛋白H4、H3乙酰化及H3K9甲基化脩飾水平的改變伴隨著mRNA水平的變化。TOPOⅡα啟動子調控因子Sp1可能通過組蛋白H4、H3乙酰化及H3K9甲基化脩飾改變在苯中毒所緻的造血毒性中髮揮作用。
목적：연구탁복이구매Ⅱα（ TOPOⅡα）계동자조공인자SP1조단백화학수식재분중독병인중적개변。방법：25례림상만성분중독환자골수단개핵세포위병례조，25례정상인골수단개핵세포위대조조，염색질면역침정기술탐토TOPOⅡα계동자조공인자Sp1조단백을선화화갑기화수평적변화，RT－PCR법측정Sp1 mRNA적표체수평。결과：여대조조상비，림상분중독병례TOPOⅡα계동자조공인자Sp1조단백H4화H3을선화수평하강（P＜0．01），조단백H3K9갑기화수평승고（P＜0．01），조단백H3K4갑기화수평무명현개변。여정상대조조상비，림상분중독병례TOPOⅡα계동자조공인자Sp1적mRNA표체수평강저（ P＜0．05）。결론：만성분중독TO－POⅡα계동자조공인자Sp1조단백H4、H3을선화급H3K9갑기화수식수평적개변반수착mRNA수평적변화。TOPOⅡα계동자조공인자Sp1가능통과조단백H4、H3을선화급H3K9갑기화수식개변재분중독소치적조혈독성중발휘작용。
AIM:To investigate the histone modification changes of topoisomerase Ⅱα( TOPOⅡα) promoter regulatory factor Sp1 in the patients with chronic benzene poisoning .METHODS:The bone marrow samples were collect-ed from 25 chronic benzene poisoning cases and 25 controls.The chromatin immunoprecipitation assay was carried out to study the possible mechanism of TOPOⅡαpromoter regulatory factor Sp 1 expression changes .The mRNA expression of Sp1 was detected by RT-PCR.RESULTS:Compared with the controls , the histone H4 acetylation and histone H3 acetyla-tion of Sp1 in the chronic benzene poisoning patients significantly decreased (P<0.01), and histone H3K9 methylation level of Sp1 increased (P<0.01), but the histone H3K4 methylation level of SP1 was not obviously changed (P>0.05). The mRNA expression of Sp1 in the chronic benzene poisoning patients was significantly lower than that in the controls (P<0.05).CONCLUSION:In chronic benzene poisoning patients , the histone acetylation and methylation modification changes of TOPOⅡαpromoter regulatory factor Sp 1 accompanied with the changes of mRNA level are observed .Histone H4 and H3 acetylation and H3K9 methylation modification of Sp1 may play an important role in the benzene ’s hematopoiet-ic toxicities.
