医学检验与临床
醫學檢驗與臨床
의학검험여림상
Medical Laboratory Science and Clinics
2015年
4期
9-11
,共3页
乙肝病毒%肝炎%乙型%慢性%肝肿瘤%微RNA
乙肝病毒%肝炎%乙型%慢性%肝腫瘤%微RNA
을간병독%간염%을형%만성%간종류%미RNA
Hepatitis B virus%Hepatitis B%Chronic%HCC%MiRNA
目的：探讨乙型肝炎病毒感染相关肝病中miRNA139的表达情况及其临床意义。方法：应用实时荧光定量PCR方法检测慢性乙型肝炎（CHB）患者（20例）、慢性乙肝肝硬化（LC）患者（20例）、原发性肝癌（HCC）患者（20例）及正常对照(NC)（20例）组外周血单核细胞中miRNA139的表达水平，结合临床指标进行分析。结果：与NC组比较，CHB组和LC组患者外周血中miRNA139的表达水平下调不明显，没有显著差异（P>0.05）；与NC组、LC组和CHB组比较，HCC组患者外周血中miRNA139的表达水平明显下调，差异具有统计学意义（P＜0.01）。HCC患者外周血中miRNA139的表达与AFP呈负相关。结论：HCC组患者外周血中miRNA139的表达水平明显下降，提示miRNA139的下调可能与HCC的发生有关。
目的：探討乙型肝炎病毒感染相關肝病中miRNA139的錶達情況及其臨床意義。方法：應用實時熒光定量PCR方法檢測慢性乙型肝炎（CHB）患者（20例）、慢性乙肝肝硬化（LC）患者（20例）、原髮性肝癌（HCC）患者（20例）及正常對照(NC)（20例）組外週血單覈細胞中miRNA139的錶達水平，結閤臨床指標進行分析。結果：與NC組比較，CHB組和LC組患者外週血中miRNA139的錶達水平下調不明顯，沒有顯著差異（P>0.05）；與NC組、LC組和CHB組比較，HCC組患者外週血中miRNA139的錶達水平明顯下調，差異具有統計學意義（P＜0.01）。HCC患者外週血中miRNA139的錶達與AFP呈負相關。結論：HCC組患者外週血中miRNA139的錶達水平明顯下降，提示miRNA139的下調可能與HCC的髮生有關。
목적：탐토을형간염병독감염상관간병중miRNA139적표체정황급기림상의의。방법：응용실시형광정량PCR방법검측만성을형간염（CHB）환자（20례）、만성을간간경화（LC）환자（20례）、원발성간암（HCC）환자（20례）급정상대조(NC)（20례）조외주혈단핵세포중miRNA139적표체수평，결합림상지표진행분석。결과：여NC조비교，CHB조화LC조환자외주혈중miRNA139적표체수평하조불명현，몰유현저차이（P>0.05）；여NC조、LC조화CHB조비교，HCC조환자외주혈중miRNA139적표체수평명현하조，차이구유통계학의의（P＜0.01）。HCC환자외주혈중miRNA139적표체여AFP정부상관。결론：HCC조환자외주혈중miRNA139적표체수평명현하강，제시miRNA139적하조가능여HCC적발생유관。
Objective:To investigate the expressions and clinical significance of microRNA(miRNA)139 in the peripheral blood mononuclear cells(PBMCs)of patients with Hepatitis B. Methods:The levels of miRNA139 in PBMCs of patients with CHB, LC,HCC and normal controls were detected by fluorescence real-time quantitative PCR method and combined with clinical correlation analysis.Results:There was no statistical difference in the miRNAl39 expression in PBMCs between patients with CHB,LC and normal controls .The levels of miRNA139 in PBMCs of patients with HCC were lower than that of normal controls and patients with CHB,LC(P<0.01).Negative correlation was found between the miRNA 139 and AFP in HCC patients,but no correlation with HBV-DNA or ALT. Conclusions:The reduction of miRNA 139 expression related to the incidence of HCC. Detection of miRNA 139 might help in diagnosis and prediction of prognosis of HCC.
