CAJ | 학술논문

研究人二倍体细胞甲型肝炎灭活疫苗的纯化方法。经人二倍体细胞培养的病毒液经澄清、浓缩后制成粗制疫苗，采用Sephacryl S-400、DEAE-Sepharose FF、Sephadex G-10等柱层析手段进行纯化。试验证明甲型肝炎病毒粗制疫苗经以上3种层析柱纯化后，抗原组分单一，收率达到85％，杂蛋白去除率达85％以上，蛋白总含量、牛血清残留量各项指标符合中国药典要求，适用于大规模生产。
연구인이배체세포갑형간염멸활역묘적순화방법。경인이배체세포배양적병독액경징청、농축후제성조제역묘，채용Sephacryl S-400、DEAE-Sepharose FF、Sephadex G-10등주층석수단진행순화。시험증명갑형간염병독조제역묘경이상3충층석주순화후，항원조분단일，수솔체도85％，잡단백거제솔체85％이상，단백총함량、우혈청잔류량각항지표부합중국약전요구，괄용우대규모생산。
Purification method of human diploid cell hepatitis A inactivated vaccine was studied.Liquid of hepatitis A virus cultured with human diploid cell was levigated and enriched to prepare crude vaccine.Then it was purified by means of Sephacryl S-400, DEAE-Sepharose FF, and Sephadex G-10 column chromatography.The results showed that the antigen of hepatitis A virus purified by three kinds of column chromatography mentioned above, the component of antigen was singular with recovery rate as high as 85%, and the depletion of the hetero-protein was over 85%.The content of total protein and various indices of BSA residue were all suitable to the Pharmacopoeia of the People’ s Re-public of China, and it is appropriate to produce in large scale.