微生物学杂志
微生物學雜誌
미생물학잡지
Journal of Microbiology
2015年
3期
71-74
,共4页
毕赤酵母%启动子%蛋白表达
畢赤酵母%啟動子%蛋白錶達
필적효모%계동자%단백표체
Pichia pastoris%promoter%protein expression
甲醇营养型毕赤酵母是一个广泛使用的蛋白表达宿主系统,易于高密度发酵、具有真核细胞翻译后加工修饰特点,适于异源蛋白分泌表达。转录调控是控制蛋白高效表达的关键环节,启动子是其中重要的元件。毕赤酵母表达系统中应用最为广泛的是甲醇诱导型AOX1启动子和组成型的GAP启动子,已成功用于一些异源蛋白的表达。近年来,发现了其他一些可供利用的启动子,包括来自管家基因的启动子如TEF、PGK1,以及具有特殊调控机制的启动子如FLD、PHO89等。此外,通过对启动子进行序列改造,构建启动子文库,实现了对启动子的精细调控。不同的启动子具有各自独特的调控机制与特点,就毕赤酵母启动子在异源蛋白表达应用中的相关研究进展进行综述。
甲醇營養型畢赤酵母是一箇廣汎使用的蛋白錶達宿主繫統,易于高密度髮酵、具有真覈細胞翻譯後加工脩飾特點,適于異源蛋白分泌錶達。轉錄調控是控製蛋白高效錶達的關鍵環節,啟動子是其中重要的元件。畢赤酵母錶達繫統中應用最為廣汎的是甲醇誘導型AOX1啟動子和組成型的GAP啟動子,已成功用于一些異源蛋白的錶達。近年來,髮現瞭其他一些可供利用的啟動子,包括來自管傢基因的啟動子如TEF、PGK1,以及具有特殊調控機製的啟動子如FLD、PHO89等。此外,通過對啟動子進行序列改造,構建啟動子文庫,實現瞭對啟動子的精細調控。不同的啟動子具有各自獨特的調控機製與特點,就畢赤酵母啟動子在異源蛋白錶達應用中的相關研究進展進行綜述。
갑순영양형필적효모시일개엄범사용적단백표체숙주계통,역우고밀도발효、구유진핵세포번역후가공수식특점,괄우이원단백분비표체。전록조공시공제단백고효표체적관건배절,계동자시기중중요적원건。필적효모표체계통중응용최위엄범적시갑순유도형AOX1계동자화조성형적GAP계동자,이성공용우일사이원단백적표체。근년래,발현료기타일사가공이용적계동자,포괄래자관가기인적계동자여TEF、PGK1,이급구유특수조공궤제적계동자여FLD、PHO89등。차외,통과대계동자진행서렬개조,구건계동자문고,실현료대계동자적정세조공。불동적계동자구유각자독특적조공궤제여특점,취필적효모계동자재이원단백표체응용중적상관연구진전진행종술。
Methylotrophic Pichia pastoris is a host system widely used for protein expression.With its properties of easily fermented in high density and processing modification after eukaryotic cells interpretation, it is suitable for the expression of heterogeneous protein ( Hp) .Transcriptional regulation is a crucial link for efficient protein expression, and promoter is the most important element.Methanol inducible promoter AOX1 and constitutive promoter GAP are the most widely used promoters in P.pastoris expression system, which had been successfully used for expressing Hp. Recently, some alternative promoters have been found, including the promoters from housekeeping gene e.g.TEF, PGK1, and the promoters having the special regulation characterization e.g.FLD, PHO89, and so on.Through pro-moter sequence transformation, construction of promoter library has realized fine regulation of promoters.Different promoters individually have their specific regulation mechanisms and regulation characteristics.The application of P. pastoris promoters for expressing Hp in related research progress were reviewed in this paper.